Recombinant Human Probable ATP-dependent RNA helicase DDX53 (DDX53)

Code CSB-YP773024HU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP773024HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP773024HU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP773024HU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
DDX53
Uniprot No.
Alternative Names
CAGE; Cancer associated gene protein; Cancer-associated gene protein; Cancer/testis antigen 26; CT26; DDX53; DDX53_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 53; DEAD box protein 53; DEAD box protein CAGE; Probable ATP dependent RNA helicase DDX53; Probable ATP-dependent RNA helicase DDX53
Species
Homo sapiens (Human)
Expression Region
1-631
Target Protein Sequence
MSHWAPEWKR AEANPRDLGA SWDVRGSRGS GWSGPFGHQG PRAAGSREPP LCFKIKNNMV GVVIGYSGSK IKDLQHSTNT KIQIINGESE AKVRIFGNRE MKAKAKAAIE TLIRKQESYN SESSVDNAAS QTPIGRNLGR NDIVGEAEPL SNWDRIRAAV VECEKRKWAD LPPVKKNFYI ESKATSCMSE MQVINWRKEN FNITCDDLKS GEKRLIPKPT CRFKDAFQQY PDLLKSIIRV GIVKPTPIQS QAWPIILQGI DLIVVAQTGT GKTLSYLMPG FIHLDSQPIS REQRNGPGML VLTPTRELAL HVEAECSKYS YKGLKSICIY GGRNRNGQIE DISKGVDIII ATPGRLNDLQ MNNSVNLRSI TYLVIDEADK MLDMEFEPQI RKILLDVRPD RQTVMTSATW PDTVRQLALS YLKDPMIVYV GNLNLVAVNT VKQNIIVTTE KEKRALTQEF VENMSPNDKV IMFVSQKHIA DDLSSDFNIQ GISAESLHGN SEQSDQERAV EDFKSGNIKI LITTDIVSRG LDLNDVTHVY NYDFPRNIDV YVHRVGYIGR TGKTGTSVTL ITQRDSKMAG ELIKILDRAN QSVPEDLVVM AEQYKLNQQK RHRETRSRKP GQRRKEFYFL S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Gene References into Functions
  1. A novel role of DDX53 in regulating cancer stem cell-like properties by binding to SOX-2.DDX53 directly regulates the SOX-2 expression in drug-resistant melanoma cells. PMID: 28535666
  2. DDX53 promotes stem cell-like properties, autophagy, and confers resistance to anti-cancer drugs in breast cancer cells. PMID: 28152297
  3. Results provide evidence that miR-30a forms a positive feedback loop with CAGE to regulate the expression of p53 confering resistance to anti-cancer drugs. PMID: 26912082
  4. miR-217-CAGE feedback loop serves as a target for overcoming resistance to various anti-cancer drugs, including EGFR and HER2 inhibitors. PMID: 26863629
  5. Results show the direct regulation of CAGE expression by HDAC3 and that HDAC3-CAGE axis regulates the activation of EGFR. HDAC3 targets CAGE to regulate the tumorigenic potential and angiogenic potential of cancer cells. PMID: 26883907
  6. CAGE induced the interaction between histone deacetylase 2 (HDAC2) and Snail, which exerted a negative effect on p53 expression in neoplastic cells; CAGE confers drug resistance by regulating expression of p53 through HDAC2. PMID: 20534591
  7. CAGE is expressed in a variety of cancers but not in normal tissues except testis, and might play a role in cellular proliferation. PMID: 11922625
  8. results suggest that the methylation status of the CpG sites of CAGE determines its expression, and that hypomethylation of CAGE precedes the development of gastric cancer and hepatocellular carcinoma PMID: 12849980
  9. CAGE promotes motility of cancer cells through activation of focal adhesion kinase (FAK). PMID: 17028776
  10. Hypomethylated CAGE promoter CpG islands are frequently involved in uterine cervical carcinogenesis. PMID: 17341616
  11. The CAGE gene is widely expressed in various cancer tissues and cancer cell lines. PMID: 18388483

Show More

Hide All

Subcellular Location
Nucleus.
Protein Families
DEAD box helicase family
Tissue Specificity
Expressed in testis. Wide expression in various cancer tissues and cancer cell lines.
Database Links

HGNC: 20083

KEGG: hsa:168400

STRING: 9606.ENSP00000368667

UniGene: Hs.434416

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1