Recombinant Human Probable E3 ubiquitin-protein ligase HERC4 (HERC4), partial

Code CSB-YP682930HU
MSDS
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Source Yeast
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Code CSB-EP682930HU
MSDS
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Source E.coli
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Code CSB-EP682930HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP682930HU
MSDS
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Source Baculovirus
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Code CSB-MP682930HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HERC4
Uniprot No.
Alternative Names
DKFZP564G092; HECT and RLD domain containing E3 ubiquitin protein ligase 4; HECT domain and RCC1 like domain containing protein 4; HECT domain and RCC1-like domain-containing protein 4; Hect domain and RLD 4; HERC 4; HERC4; HERC4_HUMAN; KIAA1593; Probable E3 ubiquitin protein ligase HERC4; Probable E3 ubiquitin-protein ligase HERC4
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
Gene References into Functions
  1. HERC4 has an effect on the occurrence and development of hepatoma carcinoma PMID: 28430527
  2. High HERC4 expression is associated with lung cancer. PMID: 25684480
  3. HERC4 ancestor emerged in nematodes and that the family expanded throughout evolution. The mRNA expression pattern of the HERC4 was found to be diverse in selected tissues and cells PMID: 15676274
Subcellular Location
Cytoplasm, cytosol. Note=shows a punctate cytoplasmic distribution.
Tissue Specificity
Expressed in brain and testis and detected in heart and placenta.
Database Links

HGNC: 24521

OMIM: 609248

KEGG: hsa:26091

STRING: 9606.ENSP00000378624

UniGene: Hs.51891

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