Recombinant Human Probable G-protein coupled receptor 133 (GPR133), partial

Code CSB-YP744167HU
MSDS
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Source Yeast
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Code CSB-EP744167HU
MSDS
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Source E.coli
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Code CSB-EP744167HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP744167HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP744167HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ADGRD1
Uniprot No.
Alternative Names
DKFZp434B1272; FLJ16770; G protein coupled receptor 133; G protein coupled receptor PGR25; G-protein coupled receptor PGR25; GP133_HUMAN; Gpr133; MGC138512; MGC138514; PGR25; Probable G-protein coupled receptor 133
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Orphan receptor. Signals via G(s)-alpha family of G-proteins. Has protumorigenic function especially in glioblastoma.
Gene References into Functions
  1. The results show that a broad spectrum of functionally relevant ADGRD1 variants is present in the human population which may cause clinically relevant phenotypes, while being compatible with life when heterozygous. PMID: 27516204
  2. Cell adhesion receptor GPR133 couples to Gs protein PMID: 22025619
  3. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
  4. A role for GPR133 protein in affecting the length of the electrocardiographic RR interval and heart rate. PMID: 20031603
  5. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 20031603
  6. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19729412

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily
Tissue Specificity
Up-regulated in CD133(+) cell population of glioblastoma.
Database Links

HGNC: 19893

OMIM: 613639

KEGG: hsa:283383

STRING: 9606.ENSP00000261654

UniGene: Hs.719239

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