Recombinant Human Probable histidine--tRNA ligase, mitochondrial (HARS2)

Code CSB-YP010138HU
MSDS
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Source Yeast
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Code CSB-EP010138HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010138HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP010138HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HARS2
Uniprot No.
Alternative Names
4631412B19Rik; AI593507; HARS related; Hars2; HARSL; HARSR; HisRS; Histidine translase; Histidine tRNA ligase; Histidine tRNA ligase homolog; Histidine tRNA ligase like; Histidine--tRNA ligase; Histidine--tRNA ligase-like; Histidyl tRNA synthetase 2; Histidyl tRNA synthetase 2 mitochondrial; Histidyl tRNA synthetase like; HO3; mitochondrial; Probable histidyl tRNA synthetase mitochondrial; Probable histidyl-tRNA synthetase; SYHM_HUMAN
Species
Homo sapiens (Human)
Expression Region
34-506
Target Protein Sequence
QSQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGM DTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHV GKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVN DRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQ CHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYT GVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYI VEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQL HYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mitochondrial aminoacyl-tRNA synthetase that catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP).
Gene References into Functions
  1. Mutations in mitochondrial histidyl tRNA synthetase HARS2 cause ovarian dysgenesis and sensorineural hearing loss of Perrault syndrome. PMID: 21464306
  2. This protein has been found differentially expressed in the anterior cingulate cortex in men patients with schizophrenia. PMID: 20381070
Involvement in disease
Perrault syndrome 2 (PRLTS2)
Subcellular Location
Mitochondrion.
Protein Families
Class-II aminoacyl-tRNA synthetase family
Tissue Specificity
A high level expression is seen in the heart, kidney and skeletal muscle while a lower level expression is seen in the brain and liver.
Database Links

HGNC: 4817

OMIM: 600783

KEGG: hsa:23438

STRING: 9606.ENSP00000230771

UniGene: Hs.432560

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