Recombinant Human Proline-, glutamic acid- and leucine-rich protein 1 (PELP1), partial

Code CSB-YP815592HU
MSDS
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Source Yeast
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Code CSB-EP815592HU
MSDS
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Source E.coli
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Code CSB-EP815592HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP815592HU
MSDS
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Source Baculovirus
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Code CSB-MP815592HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PELP1
Uniprot No.
Alternative Names
glutamic acid- and leucine-rich protein 1; HMX3; MNAR; Modulator of non-genomic activity of estrogen receptor; Modulator of nongenomic activity of estrogen receptor; P160; Pelp1; PELP1 proline glutamic acid leucine rich protein 1; PELP1_HUMAN; proline and glutamic acid rich nuclear protein; Proline glutamate and leucine rich protein 1; Proline-; proline-, glutamic acid- and leucine-rich protein 1; Transcription factor HMX3
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit. Regulates pre-60S association of the critical remodeling factor MDN1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K.
Gene References into Functions
  1. High PELP1 expression is associated with inflammation and breast cancer. PMID: 27881676
  2. three techniques for the first time demonstrated the presence of the PELP1 in paraspinal muscles of patients with idiopathic scoliosis. PMID: 27045366
  3. PELP1 interacted with GR to activate Brk expression. PMID: 26825173
  4. we propose that PELP1/Ki-67 double high expression in tumors is an independent prognostic factor for predicting a poor outcome for patients with Triple-negative breast cancer PMID: 26472563
  5. There is mounting evidence that PELP1 plays key roles in several biological processes and its deregulation leads to the development of cancers. PMID: 26158753
  6. There are significantly higher amounts of PELP1 transcript and protein in tumor tissues in patients with non-small cell lung cancer. PMID: 26211588
  7. The diffuse and strong nuclear immunoreactivity of PELP1 in most cases suggests that PELP1 may be a molecular target for the treatment of triple-negative breast carcinoma PMID: 26428280
  8. Results show that PELP1 is highly phosphorylated in triple negative breast cancer cells and interacts with mutated Tp53 and E2F1 to differentially regulates their target gene expression. PMID: 25788226
  9. The PELP1 interacts with p53, functions as p53-coactivator and is required for optimal activation of p53 target genes under genomic stress. PMID: 24786831
  10. unliganded PR-B enhanced proliferative responses to estradiol and IGF1 via scaffolding of ER-alpha/PELP1/IGF1R-containing complexes PMID: 24469035
  11. our data identified the PELP1-mTOR axis as a novel component of PELP1 oncogenic functions and suggest that mTOR inhibitor(s) will be effective chemotherapeutic agents for downregulating PELP1 oncogenic functions. PMID: 24688046
  12. This review focuses on the interactive functions and therapeutic and prognostic significance of PELP1 in breast and prostate cancers. PMID: 24859989
  13. PELP1 exhibits an oncogenic function in colorectal cancer through c-Src upregulation. PMID: 24967003
  14. Overexpression of PELP1 in Chinese women with primary breast cancer appears to be associated with biomarkers of poor outcome; these results are similar to other reports based on Western populations. PMID: 24627205
  15. PELP1 oncogenic functions involve alternative splicing via PRMT6. PMID: 24447537
  16. PELP1 regulates tumor metastasis by controlling the expression and functions of the tumor metastasis suppressors miR-200a and miR-141 PMID: 23975430
  17. MacroH2A1 specifically recruits PELP1 to the promoters of macroH2A1 target genes, but macroH2A1 occupancy occurs independent of PELP1. This recruitment allows macroH2A1 and PELP1 to cooperatively regulate gene expression outcomes. PMID: 24752897
  18. results suggest that PELP1/MNAR plays a role in endometrial cancer progression and metastasis PMID: 22992812
  19. PELP1 may enable estradiol-induced androgen receptor (AR) signaling by forming a protein complex between AR, estrogen receptor beta, and PELP1 on the DNA, leading to the proliferation of prostate cancer cells in the absence of androgen. PMID: 22403175
  20. PELP1 may be a potential therapeutic target for metastatic ER-negative breast cancer. PMID: 22086908
  21. Univariate survival analysis revealed that high PELP1 expression was associated with worse prognosis in astrocytic brain tumors PMID: 21735116
  22. proto-oncogene PELP1 plays a vital role in rDNA transcription PMID: 21695158
  23. Results suggest that PELP1 signaling axis is a potential druggable target and liposomal PELP1-siRNA-DOPC could be used as a novel drug to prevent or treat ovarian metastasis. PMID: 21421858
  24. These data define the PELP1-TEX10-WDR18 complex as a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation. PMID: 21326211
  25. PELP1 protein expression is an independent prognostic predictor of shorter breast-cancer-specific survival and disease-free interval in breast cancer. Its elevated expression is positively associated with markers of poor outcome. PMID: 19495959
  26. PELP1 regulation of aromatase P450 (Cyp19), represent a novel mechanism for in situ estrogen synthesis leading to tumor proliferation by autocrine loop and open a new avenue for ablating local aromatase activity in breast tumors. PMID: 19800002
  27. PELP1, a steroid coactivator, plays a permissive role in E2-mediated cell cycle progression, presumably via its regulatory interaction with the pRb pathway PMID: 12682072
  28. Results provide novel insights about the transcription regulation of PELP1 and suggest that PELP1 participates in chromatin remodeling activity via displacement of histone 1 in cancer cells. PMID: 15374949
  29. PELP1 recruits HDAC2 and masks histones using two separate domains PMID: 15456770
  30. PELP1 expression and localization are widely deregulated in endometrial cancers. PELP1 and ERbeta were localized predominantly in cytoplasm of high-grade endometrial tumors. PELP1 may play essential role in proliferation of cancerous endometrial cells. PMID: 15579769
  31. PELP1 / MNAR and estrogen receptor beta are coexpressed in most salivary duct carcinoma and may play a role in the pathobiology of these tumors. PMID: 16021574
  32. Results suggest that the altered localization of PELP1 modulates sensitivity to antiestrogens, potentiates tumorigenicity via the activation of MAPK and AKT, and enhances cross-regulation of ER transactivation activity by growth factors. PMID: 16140940
  33. Presence of MNAR in the cell nucleus indicates that it may play a role in regulation of gene expression. PMID: 16297421
  34. role of HRS in up-regulating MAPK, presumably involving interaction with PELP1. PMID: 16352611
  35. PELP1/MNAR increases estradiol-mediated cell proliferation and participates in estradiol mediated tumorigenesis and metastasis. [REVIEW] PMID: 16826428
  36. PELP1/MNAR may contribute to the tumorigenic potential of cancer cells by serving as a scaffolding protein that couples various signaling complexes with Estrogen Receptors (review) PMID: 17128415
  37. PELP1 functions as a molecular adaptor, coupling FHL2 with nuclear receptors, and PELP1-FHL2 interactions may have a role in prostate cancer progression. PMID: 17192406
  38. Data suggest that PELP1 is a potential oncogene, that its expression is deregulated during cancer progression, and that PELP1 may play a role in oncogenesis. PMID: 17545633
  39. PELP1 and HRS reallocate to autophagosomes in response to resveratrol treatment, which might be important in the process of autophagy in the cancer cells. PMID: 17804729
  40. PELP1 signaling and its interactions with growth factor signaling components and orphan nuclear reactors may locally influence production of estradiol and promote tumor growth by promoting estrogen receptor autocrine signaling. PMID: 18079323
  41. MNAR plays an important role in ERa activation of Src/MAPK and PI3K/Akt signaling pathways [review] PMID: 18261753
  42. growth factor signals promote phosphorylation of ER coactivator PELP1 via PKA pathway, and such modification may have functional implications in breast tumors with deregulated growth factor signaling PMID: 18505929
  43. Overexpression of PELP1 is associated with ovarian tumorigenesis PMID: 18559538
  44. MNAR interacts with glucocorticoid receptor(GR) in the nucleus but not cytoplasm and regulates GR transactivation in a complex manner depending on cell type. MNAR is capable of regulating both AF1 and AF2 functions of the GR independently. PMID: 18682536
  45. Statistical analysis revealed an increase in PELP1 expression in myofibroblasts from normal mucosa through adenomas to colorectal carcinomas. PMID: 19048289
  46. ERalpha is rarely expressed while ERbeta and PELP1/MNAR are involved in colorectal tumorigenesis. PMID: 19478391
  47. PELP1 is a coactivator of estrogen receptor (ER), interacts with ER, and may have a role in mammary tumorigenesis. PMID: 11481323

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Subcellular Location
Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Cytoplasm.
Protein Families
RIX1/PELP1 family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 30134

OMIM: 609455

KEGG: hsa:27043

STRING: 9606.ENSP00000301396

UniGene: Hs.744899

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