Code | CSB-RA999292A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
B cells were irritatingly produced with a synthetic peptide derived from human PELP1 and then fused with myeloma cells to create hybridomas. The variable light (VL) and variable heavy (VH) domains of PELP1 antibody-producing hybridomas were sequenced, which served as a blueprint for the construction of a vector for recombinant production. The PELP1 monoclonal antibody gene-carrying vector was transfected into cells, and the PELP1 recombinant monoclonal antibody was extracted and purified using affinity chromatography from the cell culture supernatant. The purified antibody was then assessed for specificity using ELISA, WB, and IHC applications and it was found to exclusively target human PELP1 protein.
The PELP1 protein plays a role in multiple cellular processes. It acts as a transcriptional co-activator, meaning that it helps to activate the expression of certain genes. It is also involved in protein-protein interactions and may help to regulate signaling pathways involved in cell growth and differentiation. In addition, PELP1 has been linked to hormone receptor signaling, with some studies suggesting that it may play a role in breast cancer development and progression.
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