Recombinant Human Prostaglandin G/H synthase 2 (PTGS2), partial

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Code CSB-EP018986HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018986HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) PTGS2.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018986HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) PTGS2.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
PTGS2
Uniprot No.
Research Area
Metabolism
Alternative Names
COX 2; COX-2; COX2; Cyclooxygenase 2; Cyclooxygenase 2b; Cyclooxygenase; Cyclooxygenase-2; Cyclooxygenase2; EC 1.14.99.1; fj02a10; Glucocorticoid-regulated inflammatory cyclooxygenase; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase ; GRIPGHS; hCox 2; Macrophage activation-associated marker protein P71/73; OTTHUMP00000033524 ; PES-2; PGG/HS ; PGH synthase 2; PGH2_HUMAN; PGHS 2; PGHS-2; PGHS2; PHS 2; PHS II; PHS2; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) ; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2 precursor ; Prostaglandin G/H synthase and cyclooxygenase ; Prostaglandin G/H synthase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) ; Prostaglandin-endoperoxide synthase 2; PTGS2; ptgs2a; TIS10; TIS10 protein; unp1239; wu:fj02a10
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
18-601aa
Target Protein Sequence
ANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCSTPEFLTRIKLFLKPTPNTVHYILTHFKGFWNVVNNIPFLRNAIMSYVLTSRSHLIDSPPTYNADYGYKSWEAFSNLSYYTRALPPVPDDCPTPLGVKGKKQLPDSNEIVEKLLLRRKFIPDPQGSNMMFAFFAQHFTHQFFKTDHKRGPAFTNGLGHGVDLNHIYGETLARQRKLRLFKDGKMKYQIIDGEMYPPTVKDTQAEMIYPPQVPEHLRFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNKQFQYQNRIAAEFNTLYHWHPLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
70.9kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The expression region of this recombinant Human PTGS2 covers amino acids 18-601. The calculated molecular weight for this PTGS2 protein is 70.9 kDa. This PTGS2 recombinant protein is manufactured in e.coli. The PTGS2 coding gene included the N-terminal 6xHis tag, which simplifies the detection and purification processes of the recombinant PTGS2 protein in following stages of expression and purification.

Prostaglandin G/H synthase 2 (PTGS2) is an enzyme involved in the synthesis of prostaglandins, which are lipid signaling molecules with various physiological effects. The main function of PTGS2 is to convert arachidonic acid into prostaglandin H2 (PGH2), a precursor for the synthesis of various prostaglandins. PTGS2 plays a crucial role in inflammation, as it is often induced in response to inflammatory stimuli. It is involved in the regulation of immune responses, cell proliferation, and tissue repair. Dysregulation of PTGS2 has been implicated in various pathological conditions, including cancer, inflammatory disorders, and cardiovascular diseases. Research areas related to PTGS2 encompass understanding its role in inflammation, elucidating its contribution to different disease states, and exploring its potential as a therapeutic target.

Customer Reviews and Q&A

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 Q&A
Q:

I asks for the composition of the buffer for item CSB-EP018986HU. I need to know every component of the buffer.

A:
Very nice to receive your inquiry.
The buffer is as below: 10 mM Tris-HCl, 1 mM EDTA, pH8.0. As for the final concentration of glycerol, in principle, the final concentration from 5% to 50% are all OK, which may influence storage time.
If glycerol content is higher, relatively speaking, protein storage time will be longer. However, it all depend on your decision, adding glycerol or not adding glycerol, adding how much glycerol.
Q:

We are interested in several small units of many different products.  However,we are only interested in products with a GST tag.  For those listed below that have a His tag, what is the expense and time associated with getting these in a GST tag?   CSB-EP018986HU His

A:
Thanks for your inquiry,Note: We use different codes for different tags now, so the codes of these proteins have been updated below.
Code: CSB-EP018986HUe0
Name: Recombinant Human Prostaglandin G/H synthase 2(PTGS2),partial
Expression Region: 18-601aa, Partial
Tag Info: N-terminal GST-tag
Expression Sequence:

ANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCSTPEFLTRIKLFLKPTPNTVHYILTHFKGFWNVVNNIPFLRNAIMSYVLTSRSHLIDSPPTYNADYGYKSWEAFSNLSYYTRALPPVPDDCPTPLGVKGKKQLPDSNEIVEKLLLRRKFIPDPQGSNMMFAFFAQHFTHQFFKTDHKRGPAFTNGLGHGVDLNHIYGETLARQRKLRLFKDGKMKYQIIDGEMYPPTVKDTQAEMIYPPQVPEHLRFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNKQFQYQNRIAAEFNTLYHWHPLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERS


Shipping format: The default delivery form is liquid form. If the customer has demand for lyophilized form, please remark this requirement when placing order.
Optional service: Do you want aseptic processing and/or endotoxin removal for this protein (recommended for cell culture or in vivo use)? With our endotoxin removal service we guarantee the endotoxin level to be
less than 0.1ng/ug (1EU ug). Both aseptic processing and endotoxin removal services are free of charge however they must be requested when the order is placed.
Purity: Greater than 90% as determined by SDS-PAGE.

Target Background

Function
Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia.
Gene References into Functions
  1. SND1 may act as a potential biomarker of the therapeutic strategies utilizing COX2 inhibitors. PMID: 30365124
  2. Authors showed that mRNA and protein levels of COX2 and HER2 were upregulated in CRC compared with the adjacent tissues. COX2 protein levels and nuclear COX2 expression were correlated with a poor prognosis of CRC patients. COX2 expression was positively associated with HER2 expression. PMID: 29873317
  3. PTGS2 polymorphisms were associated with advanced liver fibrosis in patients with HCV mono-Infection and HCV/HIV Co-Infection. PMID: 30139224
  4. An unrecognized cellular interaction between follicular dendritic cells and B cells leading to COX-2 expression during immune inflammatory responses. PMID: 29241029
  5. Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in Alzheimer's disease patients and in a transgenic mouse model. PMID: 29662056
  6. miR-137 suppresses the proliferation and invasion of retinoblastoma cells by targeting COX-2/PGE2 signaling pathway. PMID: 29945115
  7. Dual governing of YAP and COX-2 may lead to the discovery of promising therapeutic strategies for HCC patients. PMID: 29505957
  8. COX-2 expression was positively associated with the recurrence and a poor prognosis of patients with nasopharyngeal carcinoma. PMID: 29956730
  9. COX-2 is an important factor for Dengue virus replication. PMID: 28317866
  10. Lysophosphatidylcholine induces COX-2-mediated IL-6 expression. NADPH oxidase/Reactive Oxygen Species is involved in Lysophosphatidylcholine-induced COX-2 expression. PMID: 30229288
  11. results support a critical role of ATF6alpha in the establishment and maintenance of cellular senescence in normal human fibroblasts via the up-regulation of a COX2/PGE2 intracrine pathway. PMID: 28803844
  12. High PTGS2 expression is associated with Breast Carcinoma. PMID: 30051683
  13. concluded that COX-2 gene rs5275 variant contributes to Nasopharyngeal carcinoma risk in a Chinese population PMID: 30087034
  14. COX2 and YAP1 signaling pathways are connected with each other to induce SOX2 expression, cancer stem cell enrichment, and acquired resistance to chemotherapy in urothelial carcinoma of the bladder. PMID: 29180467
  15. No significant association between COX-2 8473 T > C polymorphism and cancer risk was detected. PMID: 30143023
  16. The results revealed that TLR4 and COX-2 were upregulated in PCa tissues; silencing of TLR4 or COX-2 inhibited PCa cell proliferation, migration, and invasion. PMID: 30098292
  17. Results describe a novel role for cyclooxygenase-2 (COX-2) in mediating the TGFbeta effects on breast cancer stem cells (BCSC) properties and imply that targeting the COX-2 pathway may prove useful for the treatment of triple-negative breast cancer by eliminating BCSCs. PMID: 28054666
  18. the effects of miR-101 inhibition on tumor growth were suppressed by COX-2 inhibition. PMID: 29404887
  19. Low PTGS2 expression is associated with Invasive Breast Carcinoma. PMID: 28808873
  20. rs2243250 (IL4) and rs5275 (PTGS2) were found to be significantly associated with shorter renal cell cancer-specific survival (CSS). PMID: 28117391
  21. meta-analysis demonstrated that COX-2 rs5275 and rs689466 polymorphism significantly decrease the risk of lung cancer in Asians but not in Caucasians, indicating COX-2 could serve as a potential diagnostic marker for lung cancer PMID: 30170377
  22. meta-analysis of association between 765G/C polymorphism and periodontitis in Chinese population PMID: 29514641
  23. Through downregulation of COX-2 expression in SGC-7901 and MGC-803 cells. PMID: 29901169
  24. Patients with a high expression of COX-2 in baseline tumor biopsies had less response to treatment of pathology compared to patients with lower expression of COX-2 in baseline tumor biopsies. PMID: 29893307
  25. Studied the association between integrin subunit alpha 2 (GPIa) and prostaglandin-endoperoxide synthase 2 (COX-2) genetic polymorphisms in Chinese ishemic stroke patients with or without aspirin resistance. PMID: 28948649
  26. COX-2 was significantly associated with a lower 5-year disease-free survival (DFS) rate PMID: 29559247
  27. The polymorphism in the COX2 gene is associated with increased susceptibility to colorectal cancer, specially rectosigmoid tumors PMID: 29257846
  28. demonstrate the unregulated expression of ANXA1 and COX-2 in precursor lesions of esophageal and stomach cancers PMID: 29254791
  29. The cytotoxicity induced by EB1 gene knockdown was due to the activation and generation of reactive oxygen species by p38 mitogen-activated protein kinase..this signaling cascade, however not nuclear factor-kappaB-mediated signaling, induced the expression of cyclooxygenase-2, a key effector of apoptotic death. PMID: 29484424
  30. High COX2 expression is associated with Ras and BRAF mutations in Hepatocellular Carcinoma. PMID: 28188432
  31. High COX2 expression is associated with ovarian cancer cell migration and invasion. PMID: 28677781
  32. hypothesize that lower transcript levels of PTGS2 in cumulus cells may be involved in the impairment of oocyte quality, suggesting a possible mechanism involved in disease-related infertility PMID: 28734688
  33. Results suggest that a significant correlation exists in Japan between the COX-2 1195 G-carrier genotype and intestinal metaplasia in histological and endoscopic findings based on Kyoto classification in H. pylori-infected gastric mucosa. PMID: 28946145
  34. activated Ras, protumorigenic COX-2 and Notch1 have roles in in papillary mucinous neoplasm onset PMID: 27381829
  35. TGF-beta1 increased the COX-2 and PGE2 level of cultured pulp cells. The effect of TGF-beta1 on COX-2 protein expression was associated with ALK5/Smad2/3 and MEK/ERK pathways. PMID: 28779848
  36. Culinary herbs and spices prevent the growth of HCA-7 colorectal adenocarcinoma cancer cells and inhibit their COX-2 expression. PMID: 28934138
  37. medical use of COX inhibitors in glioblastoma treatment has been limited due to their well-documented vascular toxicity and inconsistent outcomes from recent human studies. Prostaglandin E2 (PGE2) has emerged as a principal mediator for COX-2 cascade-driven gliomagenesis PMID: 28718447
  38. COX2 inversely regulated Notch1 in gastric cancer and partially depended on the Notch1 signalling pathway in altering the expression of Snail. PMID: 28586004
  39. Based on the contribution maps from the three techniques, it can be concluded that both the benzenesulfonyl group and the central five-membered ring - having a high-electronegativity functional group or atom or having a substituent hydrogen bonding acceptor - contribute positively to the selective inhibition of COX-2 PMID: 27145042
  40. Findings demonstrate that COX-2 and p-Akt1 play an important combined role during melanoma progression and are associated with highly metastatic tumors and survival rates in patients with MM. PMID: 28604419
  41. Results suggest that higher COX-2 expression may be a negative prognostic factor in conjunctival melanoma. Further studies can address the potential use of anti-COX-2 drugs as adjuvant therapy of this disease. PMID: 29297092
  42. activation of ERK1/2 signaling was required for hCG-induced up-regulation of SPRY2 expression. Further, SPRY2 knockdown attenuated the AREG-induced COX-2 expression and PGE2 production by inhibiting AREG-activated ERK1/2 signaling. PMID: 27539669
  43. COX-2 was elevated in glioma tissues and its expression was negatively correlated with the levels of miR-128. These findings may establish miR-128 as a new potential target for the treatment of patients with gliomas. PMID: 29524580
  44. Post-transcriptional regulation of COX-2 mRNAs translation by SGs indicates a role in IL-1beta-mediated catabolic response that could be therapeutically targeted in Osteoarthritis. PMID: 27271770
  45. Results show that in influenza A viruses (IAV)-infected cells, COX-2 expression is regulated. While the protein is induced at early time of infection, via recognition of IAV vRNA by RIG-I, COX-2 expression is reduced again during on-going replication due to destabilization of its mRNA by IAV-induced TTP. PMID: 27265729
  46. Our results highlight the role of COX-2 in constitutive IDO1 expression by human tumors and substantiate the use of COX-2 inhibitors to improve the efficacy of cancer immunotherapy, by reducing constitutive IDO1 expression, which contributes to the lack of T-cell infiltration in "cold" tumors, which fail to respond to immunotherapy PMID: 28765120
  47. Chinese population with GG genotype of COX-2 gene polymorphism rs689466 have higher risk to develope post-traumatic osteomyelitis. PMID: 28682162
  48. 4-Hydroxy-2-nonenal is a natural inducer of COX-2 in atherosclerosis. (Review) PMID: 28192229
  49. RhoA and COX-2 were upregulated in early gastric cancer tissues, which facilitated the proliferation and migration of gastric cancer cells. PMID: 28624843
  50. LXR gene expression was significantly increased in obese children with obstructive sleep apnea-hypopnea syndrome (OSAHS). The severity of OSAHS was positively correlated with COX-2 levels. PMID: 28676625

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Subcellular Location
Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus inner membrane; Peripheral membrane protein. Nucleus outer membrane; Peripheral membrane protein.
Protein Families
Prostaglandin G/H synthase family
Database Links

HGNC: 9605

OMIM: 600262

KEGG: hsa:5743

STRING: 9606.ENSP00000356438

UniGene: Hs.196384

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