Recombinant Human Proteasome subunit beta type-9 (PSMB9)

Code CSB-YP018887HU
MSDS
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Source Yeast
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Code CSB-EP018887HU
MSDS
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Source E.coli
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Code CSB-EP018887HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018887HU
MSDS
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Source Baculovirus
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Code CSB-MP018887HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PSMB9
Uniprot No.
Alternative Names
Beta1i; Large multifunctional peptidase 2; Large multifunctional protease 2; LMP 2; LMP2; Low molecular mass protein 2; Macropain chain 7; MGC70470; Multicatalytic endopeptidase complex chain 7; OTTHUMP00000062982; Proteasome (prosome macropain) subunit beta type 9; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2); Proteasome beta 9 subunit ; Proteasome catalytic subunit 1i; Proteasome chain 7; Proteasome related gene 2; Proteasome subunit beta 6i; Proteasome subunit beta type 9; Proteasome subunit beta type-9; Proteasome subunit beta-1i; PSB9_HUMAN; PSMB 9; PSMB6i; PSMB9; Really interesting new gene 12 protein; RING 12; RING12; RING12 protein
Species
Homo sapiens (Human)
Expression Region
21-219
Target Protein Sequence
TTIMAVEFDG GVVMGSDSRV SAGEAVVNRV FDKLSPLHER IYCALSGSAA DAQAVADMAA YQLELHGIEL EEPPLVLAAA NVVRNISYKY REDLSAHLMV AGWDQREGGQ VYGTLGGMLT RQPFAIGGSG STFIYGYVDA AYKPGMSPEE CRRFTTDAIA LAMSRDGSSG GVIYLVTITA AGVDHRVILG NELPKFYDE
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
Gene References into Functions
  1. Defective PSMB9/beta1i expression is likely to be one of the risk factors for the development of human uterine neoplasm. PMID: 28482675
  2. single nucleotide polymorphism in PSMB9 are associated with hand dermatitis PMID: 27258892
  3. Chinese females carrying the rs17587-G/G genotype in PSMB9 may increase a higher risk for Parkinson's disease. PMID: 27098790
  4. The TAP1-rs1135216 and PSMB9-rs17587 variants are significantly associated with vitiligo, and even one copy of these mutant alleles can influence the risk among Saudis. PMID: 25548428
  5. study found prevalence of LMP2-AA genotype was higher in acute myeolid leukemia (AML) patients while it was significantly lower in multiple myeloma (MM) cases than in the control subjects; results suggestLMP2 polymorphisms could play a role in the development of AML and MM PMID: 24377540
  6. Data indicate that the codon 60 Arg/His polymorphism does not significantly alter the expression and activity of beta1i among the cell lines tested and clinical samples from colon and pancreatic cancer. PMID: 24040045
  7. functional variants in PSMB9 may contribute to melanoma susceptibility. PMID: 23360169
  8. LMP2 has a role as a tumor-suppressor and may have a role in uterine leiomyosarcoma therapy PMID: 22355695
  9. Genetic polymorphisms of rs17587 of PSMB9 appeared to be associated with rheumatoid arthritis in ethnic Han Chinese from Yunnan. PMID: 23568741
  10. study reports expression of LMP2 in patients with renal oncocytomas (RO) and eosinophilic variant of chromophobe renal cell carcinomas (CHRCC-EO); all CHRCC-EO cases (7 of 7) strongly showed nuclear LMP2 staining, as opposed to only 2 of 56 ROs; suggest its use as an aid in differential diagnosis of these neoplasias PMID: 22705098
  11. LMP2 expression in nasopharyngeal carcinoma is associated with poor prognosis. PMID: 22287277
  12. The polymorphic site is unique in the Chinese population of Han nationality at the LMP2 codon 60 loci (Arg60Cys), but a lack of association with lung cancer exists. PMID: 22261389
  13. LMP2 may negatively regulate LMS independently of its role in the proteasome. PMID: 22659265
  14. This is the first study to demonstrate that LMP2 variants can affect radiographic severity in ankylosing spondylitis. PMID: 22034108
  15. Data show that LMP2 60HH variant reduces the risk to develop MS amongst Italian HLA-A*02+ females. PMID: 20174631
  16. downregulation of expression in PBMC of chronic hepatitis B patients PMID: 20300807
  17. data show that the proteasomal ss1i subunit is dysregulated in peripheral white blood cells and in inflammatory infiltrates of minor salivary glands in patients with Sjogren's syndrome PMID: 19833746
  18. a high-frequency TAP1/LMP2 promoter polymorphism in human tumors PMID: 11788900
  19. caspase inhibition led to elevated levels of LMP2 and oxidized proteins; results suggest an age-related loss in capacity to carry out apoptosis might contribute to the observed accumulation of oxidized proteins during aging and in age-related diseases. PMID: 15284441
  20. These data suggest that LMP2 contributes to IkappaBalpha degradation and p50 generation, and that inhibition of LMP2 suppresses expression and activities of MMP-2 and MMP-9 by blocking the transfer of active NF-kappaB heterodimers into the nucleus. PMID: 16222703
  21. LMP2-associated proteasome is recruited to the entire sequence of ER target genes, implicating a role for the proteasome in both transcription initiation and elongation. PMID: 16957778
  22. These data reveal a novel "feed-forward" mechanism induced by NF-kappaB which ensures that acutely synthesized IRF-1 operates in concert with NF-kappaB to amplify the immunoproteasome and antigen-processing functions of CD40. PMID: 18694960
  23. Downregulation of LMP2 is associated with acute myeloid leukaemic blasts. PMID: 19148137
  24. Obtained data suggest the LMP2 and PSMA6 gene polymorphism significance as the risk factors of essential hypertension in adolescents. PMID: 19526842

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Peptidase T1B family
Database Links

HGNC: 9546

OMIM: 177045

KEGG: hsa:5698

STRING: 9606.ENSP00000363993

UniGene: Hs.654585

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