Recombinant Human Protein Dr1 (DR1)

Code CSB-EP007175HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
DR1
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
Down regulator of transcription 1; Down regulator of transcription 1 TBP binding; Down-regulator of transcription 1; down-regulator of transcription 1; TBP-binding (negative cofactor 2); DR 1; Dr1; Dr1 protein; NC 2; NC2; NC2 beta; NC2-beta; NC2B_HUMAN; Negative co factor 2; Negative cofactor 2; Negative cofactor 2 beta; Negative cofactor 2-beta; Protein Dr1; TATA binding protein associated phosphoprotein; TATA box/binding protein associated phosphoprotein DR1; TATA-binding protein-associated phosphoprotein
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-176aa
Target Protein Sequence
ASSSGNDDDLTIPRAAINKMIKETLPNVRVANDARELVVNCCTEFIHLISSEANEICNKSEKKTISPEHVIQALESLGFGSYISEVKEVLQECKTVALKRRKASSRLENLGIPEEELLRQQQELFAKARQQQAELAQQEWLQMQQAAQQAQLAAASASASNQAGSSQDEEDDDDI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
46.3kDa
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
Gene References into Functions
  1. results suggest that DR1 are expressed in the osteosarcoma cells and inhibit the proliferation of osteosarcoma cells by the down-regulation of the ERK1/2 and PI3K-Akt pathways. PMID: 28181134
  2. Knockdown of DR1 resulted in reductions of viral RNA and protein production, demonstrating that DR1 acts as a positive host factor in influenza A virus replication. PMID: 25589657
  3. Basal core promoters control the equilibrium between negative cofactor 2 and preinitiation complexes in human cells. PMID: 20230619
  4. The physiological functions of human Dr1 include regulation of polymerase III transcription. PMID: 19965767
  5. NC2 controls TBP binding and maintenance on DNA that is largely independent of a canonical TATA sequence PMID: 15574413
  6. the initiator core promoter element antagonizes repression of TATA-directed transcription by negative cofactor NC2 PMID: 17584739
  7. Data show that NC2beta is specifically involved in neuroblastoma pathogenesis and may be considered a new neuroblastoma biomarker. PMID: 18538002
  8. ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein PMID: 18838386
  9. heterodimerization with NC2alpha masks the nuclear localization signal in NC2beta, which prevents nuclear export of the NC2 complex PMID: 19204005

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Subcellular Location
Nucleus.
Protein Families
NC2 beta/DR1 family
Database Links

HGNC: 3017

OMIM: 601482

KEGG: hsa:1810

STRING: 9606.ENSP00000359290

UniGene: Hs.348418

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