Recombinant Human Protein FAM111A (FAM111A)

Code CSB-YP853484HU
MSDS
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Source Yeast
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Code CSB-EP853484HU
MSDS
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Source E.coli
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Code CSB-EP853484HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP853484HU
MSDS
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Source Baculovirus
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Code CSB-MP853484HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FAM111A
Uniprot No.
Alternative Names
FAM111A; KIAA1895Protein FAM111A
Species
Homo sapiens (Human)
Expression Region
1-611
Target Protein Sequence
MSCKKQRSRK HSVNEKCNMK IEHYFSPVSK EQQNNCSTSL MRMESRGDPR ATTNTQAQRF HSPKKNPEDQ TMPQNRTIYV TLKVNHRRNQ DMKLKLTHSE NSSLYMALNT LQAVRKEIET HQGQEMLVRG TEGIKEYINL GMPLSCFPEG GQVVITFSQS KSKQKEDNHI FGRQDKASTE CVKFYIHAIG IGKCKRRIVK CGKLHKKGRK LCVYAFKGET IKDALCKDGR FLSFLENDDW KLIENNDTIL ESTQPVDELE GRYFQVEVEK RMVPSAAASQ NPESEKRNTC VLREQIVAQY PSLKRESEKI IENFKKKMKV KNGETLFELH RTTFGKVTKN SSSIKVVKLL VRLSDSVGYL FWDSATTGYA TCFVFKGLFI LTCRHVIDSI VGDGIEPSKW ATIIGQCVRV TFGYEELKDK ETNYFFVEPW FEIHNEELDY AVLKLKENGQ QVPMELYNGI TPVPLSGLIH IIGHPYGEKK QIDACAVIPQ GQRAKKCQER VQSKKAESPE YVHMYTQRSF QKIVHNPDVI TYDTEFFFGA SGSPVFDSKG SLVAMHAAGF AYTYQNETRS IIEFGSTMES ILLDIKQRHK PWYEEVFVNQ QDVEMMSDED L
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Single-stranded DNA-binding serine protease that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity. DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde. Protects replication fork from stalling by removing DPCs, such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesion, or poly(ADP-ribose) polymerase 1 (PARP1)-DNA complexes trapped by PARP inhibitors. Required for PCNA loading on replication sites. Promotes S-phase entry and DNA synthesis.; (Microbial infection) May directly function at replication forks, explaining why Simian virus 40 (SV40) interacts with FAM111A to overcome host range restriction.
Gene References into Functions
  1. Together, these data suggest that FAM111A may also be important in the development and function of male genitalia. 394 PMID: 24635597
  2. study would provide evidence that FAM111A is a key molecule for normal bone development, height gain, and parathyroid hormone development and/or regulation PMID: 23996431
  3. FAM111A is a replication factor required for PCNA loading. PMID: 24561620
  4. FAM111A appears to be crucial to a pathway that governs parathyroid hormone production, calcium homeostasis, and skeletal development and growth. PMID: 23684011
  5. FAM111A functions as a host range restriction factor that is specifically targeted by SV40 large T antigen PMID: 23093934

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Involvement in disease
Kenny-Caffey syndrome 2 (KCS2); Gracile bone dysplasia (GCLEB)
Subcellular Location
Nucleus. Chromosome. Cytoplasm.
Protein Families
FAM111 family
Database Links

HGNC: 24725

OMIM: 127000

KEGG: hsa:63901

UniGene: Hs.150651

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