Recombinant Human Protein GREB1 (GREB1), partial

Code CSB-YP700434HU
MSDS
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Source Yeast
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Code CSB-EP700434HU
MSDS
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Source E.coli
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Code CSB-EP700434HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP700434HU
MSDS
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Source Baculovirus
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Code CSB-MP700434HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GREB1
Uniprot No.
Alternative Names
5730583K22Rik; 9130004E13; AF180470; AU023194; Gene regulated by estrogen in breast cancer protein; Gene regulated in breast cancer 1 protein; GREB 1; Greb1; GREB1_HUMAN; KIAA0575; mKIAA0575; OTTHUMP00000180581; Protein GREB1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in estrogen-stimulated cell proliferation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers.
Gene References into Functions
  1. GREB1 is a novel progesterone-responsive gene required for endometrial stromal decidualization. PMID: 28911214
  2. The aim of this study was to determine if genetic variation within GREB1 was associated with BMD variation. PMID: 28293781
  3. Whole exome sequence analysis of two families with renal agenesis identified a single candidate gene, GREB1L. Data implicates GREB1L as a coactivator for RARs. PMID: 28739660
  4. Which were rescued by wild-type human GREB1L mRNA. PMID: 29100090
  5. A significant association between GREB1L variants and BKA. PMID: 29100091
  6. the GREB1 region on chromosome 2p25.1 and the GREB1 gene may play a role in endometriosis risk PMID: 25788566
  7. GREB1 is a critical mediator of both the estrogen-stimulated proliferation of breast cancer cells and the androgen-stimulated proliferation of prostate cancer cells PMID: 24998469
  8. The expression of Greb1 was up-regulated in mouse tumors treated with E2 and was overexpressed in human ovarian cancers relative to human ovarian surface epithelium, suggesting a role for GREB1 in human ovarian tumor progression. PMID: 24469735
  9. RAD54B and GREB1 gene polymorphisms may not be associated with PCOS in the Han Chinese population. PMID: 23876972
  10. GREB1 is one of the central estrogen-specific ER cofactors, where it functions to mediate interactions between the ER and additional proteins. PMID: 23403292
  11. GREB1 alpha mRNA/protein expression levels were increased in ectopic tissue in peritoneal endometriosis. PMID: 22884659
  12. in ER-positive breast cancer cells, LRH-1 promotes cell proliferation by enhancing ERalpha mediated transcription of target genes such as GREB-1 PMID: 22359603
  13. Tissue expression of GREB1 is associated with organ-confined prostate cancer and may constitute a gene associated with a favorable prognosis. PMID: 19945309
  14. Longer survival was associated with hypomethylation at specific CpG sites (e.g. GREB1, TGIF and TOB1) and hypermethylation in other genes (e.g. TMCO5, PTPRN and GUCY2C). PMID: 21577013
  15. Characterize a monoclonal antibody that recognizes GREB1 protein in breast cancer cells. PMID: 19842031
  16. GREB1 is critically involved in the estrogen induced growth of breast cancer cells. PMID: 15986123
  17. GREB1 is expressed in proliferating prostatic tissue and prostate cancer, is regulated by androgens, and suppression of GREB1 blocks androgen-induced growth suggesting GREB1 may be critically involved in prostate cancer proliferation. PMID: 16496412
  18. Data show that the induction of the gene regulated in breast cancer 1 (GREB1) is mediated by binding of estrogen receptor alpha to three consensus estrogen response elements spread over approximately 20 kb of upstream flanking sequences. PMID: 17463000
  19. estrogen-stimulated GREB1 transcription may involve coordinated ER binding to all three distal consensus ERE motifs PMID: 17666587
  20. GREB1 is expressed in human skin, and is dose-dependently induced by topical 17-beta-estradiol treatment PMID: 18794456
  21. Estrogen receptors are recruited at several proximal and distal estrogen response elements upstream of the GREB1 transcription unit. PMID: 15001666

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Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
GREB1 family
Tissue Specificity
Expressed in proliferating prostatic tissue and prostate cancer.
Database Links

HGNC: 24885

OMIM: 611736

KEGG: hsa:9687

STRING: 9606.ENSP00000234142

UniGene: Hs.467733

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