Recombinant Human NUT family member 1 (NUTM1), partial

Code CSB-YP773056HU
MSDS
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Source Yeast
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Code CSB-EP773056HU
MSDS
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Source E.coli
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Code CSB-EP773056HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP773056HU
MSDS
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Source Baculovirus
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Code CSB-MP773056HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NUTM1
Uniprot No.
Alternative Names
C15orf55; Chromosome 15 open reading frame 55; DKFZp434O192; MGC138683; MGC138684; Nuclear protein in testis; NUT; NUT_HUMAN; Protein NUT
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in the regulation of proliferation. Regulates TERT expression by modulating SP1 binding to TERT promoter binding sites.
Gene References into Functions
  1. Fluorescence in situ hybridization and targeted next-generation sequencing identified a CIC-NUTM1 fusion resulting from t(15;19)(q14;q13.2). In light of morphologic features that overlap with those of NC from typical anatomical sites we have seen previously, the tumor was best classified as falling within the NC spectrum rather than CIC-associated sarcoma. PMID: 29700887
  2. Review and case series found that NUT midline carcinoma has dismal prognosis. Radiotherapy and chemotherapy improves survival, but do not provide long term control except in anecdotal cases. No impact on overall survival was found based on type of molecular translocation, i.e., NUT-BRD4, NUT-BRD3 or other variants. PMID: 29356890
  3. We postulate that BRD4-NUT (B4N) complexes override the preexisting histone code with new posttranslational modifications patterns that reflect aberrant transcription and that epigenetically modulate the nucleosome environment toward the NUT-midline carcinoma (NMC) state. PMID: 27698495
  4. we also identified a complex genomic rearrangement involving the BRD4-NUT rearrangement underlying the simple t(15;19) karyotype in Nuclear protein in testis (NUT) midline carcinoma PMID: 28203693
  5. The clinical, radiographic, and histopathologic features of NUT midline carcinoma are discussed, as well as its management options. PMID: 24892275
  6. NUT gene rearrangement does not seem to be relevant in primary pulmonary carcinomas or carcinoid tumours of the lung PMID: 26341600
  7. Authors identified eight cases of primary pulmonary NMC over 4 years and, using a NUT immunohistochemistry screen, retrospectively identified one additional case from 166 consecutive biopsies of lung carcinomas lacking glandular differentiation. PMID: 26001144
  8. discovered that the translocation oncoprotein BRD4-NUT protein occupies approximately 100-200 extremely broad, cell type-specific hyperacetylated domains in the genome PMID: 26220994
  9. Thirty-seven (76%) malignant germ cell tumors were NUT positive but staining was usually of weak to moderate intensity and observed in a relatively small proportion of neoplastic cells. PMID: 25551299
  10. Data support a model in which BRD4-NUT-stimulated histone hyperacetylation recruits additional BRD4 and interacting partners to support transcriptional activation, which underlies the BRD4-NUT oncogenic mechanism in NMC. PMID: 25512383
  11. The involvement of the NSD3 methyltransferase as a component of the NUT fusion protein oncogenic complex identifies a new potential therapeutic target. PMID: 24875858
  12. Case Report: NUT midline carcinoma in the nasal cavity with BRD4-NUT-rearrangement. PMID: 24655834
  13. Four cases with the expression of P16 in NUT carcinomas with no association with human papillomavirus are being described. PMID: 24185123
  14. Determination of NUT protein expression and gene rearrangement can allow the differentiation of NUT midline carcinoma from upper-respiratory tract poorly differentiated malignant tumors PMID: 22777717
  15. immunohistochemical study of 21 cases revealed a lack of nuclear expression of NUT by undifferentiated (anaplastic) thyroid carcinomas PMID: 23701453
  16. The novel BRD4-NUT fusion in which Exon 15 of BRD4 was fused to Exon 2 of NUT encodes a functional protein that is central to the oncogenic mechanism in these cells. PMID: 23128391
  17. Only one thymic carcinoma (2.7% of thymic carcinomas or 0.68% of TETs) was found positive for NUT expression and rearrangement PMID: 22425924
  18. Case Reports: 2 pediatric cases of nuclear protein of the testis (NUT) midline carcinoma suggestive of pulmonary origin. PMID: 22301500
  19. study provides a novel mechanism by which the BRD4-NUT oncogene perturbs BRD4 functions to block cellular differentiation and to contribute to the oncogenic progression in the highly aggressive NUT midline carcinoma PMID: 21652721
  20. NUT gene rearrangement is associated with squamous cell carcinoma of the lung with long time survival. PMID: 20871268
  21. Using a patient-derived cell line, the authors show that p300 sequestration into the chromatin-bound BRD4-NUT fusion protein foci is the principal oncogenic mechanism leading to p53 inactivation. PMID: 20676058
  22. Wild type Brd4 inhibits G(1) to S progression and we also found that the Brd4-NUT fusion augments the inhibition of progression to S phase compared with wild type Brd4. PMID: 15994877
  23. BRD-NUT fusion proteins contribute to carcinogenesis by associating with chromatin and interfering with epithelial differentiation. PMID: 17934517
  24. Approximately, 20% of undifferentiated carcinomas of the upper aerodigestive tract not associated with EBV infection were found to have rearrangements of NUT by FISH. PMID: 18391746
  25. Some carcinomas of the upper aerodigestive tract have a rearrangement of the nuclear protein of the testis (NUT) gene (NUT midline carcinomas). PMID: 19550370

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Involvement in disease
A chromosomal aberration involving NUT is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with BRD4 which produces a BRD4-NUT fusion protein.
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
NUT family
Tissue Specificity
Specifically expressed in testis.
Database Links

HGNC: 29919

OMIM: 608963

KEGG: hsa:256646

STRING: 9606.ENSP00000329448

UniGene: Hs.525769

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