Recombinant Human Protein chibby homolog 1 (CBY1)

Code CSB-YP896889HU
MSDS
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Source Yeast
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Code CSB-EP896889HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896889HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP896889HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CBY1
Uniprot No.
Alternative Names
ARPP-binding protein; C22orf2; CBY; Cby1; CBY1_HUMAN; Cytosolic leucine rich protein ; Cytosolic leucine-rich protein; Golgi and endoplasmic reticulum-associated 1; HRIHFB2025; PIGEA 14; PIGEA-14; PKD2 interactor; PKD2 interactor, Golgi and endoplasmic reticulum-associated 1; Protein Chibby; Protein chibby homolog 1
Species
Homo sapiens (Human)
Expression Region
1-126
Target Protein Sequence
MPFFGNTFSP KKTPPRKSAS LSNLHSLDRS TREVELGLEY GSPTMNLAGQ SLKFENGQWI AETGVSGGVD RREVQRLRRR NQQLEEENNL LRLKVDILLD MLSESTAESH LMEKELDELR ISRKRK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibits the Wnt/Wingless pathway by binding to CTNNB1/beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.
Gene References into Functions
  1. FAM92 proteins interact with Cby1 to promote ciliogenesis via regulation of membrane-remodeling processes. PMID: 27528616
  2. chronic Cby1 knockdown in colon cancer cells may counteract tumor progression by promoting the mesenchymal-to-epithelial transition process. PMID: 28107095
  3. Cby's C-terminal domain alone binds to TC-1 with significantly greater affinity compared to full-length Cby, implying that target binding of the coiled-coil domain is affected by the flanking disordered regions. PMID: 27082063
  4. CBY1 downregulation in CML comes from reduced protein stability when bound to 14-3-3sigma adapter protein. Dissociation raises CBY1 protein levels by enhancing its stability. The ubiquitin proteasome system reduces 14-3-3sigma-bound CBY1 stability by SUMOylation. PMID: 26147002
  5. Study showed that expression of Cby protein was strongly downregulated in laryngeal squamous cell carcinoma (LSCC) tumor tissues in comparison to normal laryngeal mucosa samples. PMID: 25175341
  6. the impact of BCR-ABL1 on Chibby1, a beta catenin antagonist involved in cell differentiation and transformation PMID: 24339928
  7. Stable expression of CBY1 drives beta catenin cytoplasmatic translocation and impairs beta catenin signaling in BCR-ABL + cells. PMID: 23707389
  8. Results from the association analysis suggest that common variation in CBY is not a cause for obesity in the Belgian population. PMID: 23645032
  9. C-terminal polycystin-2 influences the interaction with PIGEA14 PMID: 23838289
  10. Cby plays an important role in organization of both primary and motile cilia in collaboration with Cnx. PMID: 22911743
  11. The N-terminal portion of the CBY1 is unstructured in solution, but the C-terminal half forms a coiled-coil structure. PMID: 21182262
  12. Chibby and clusterin were co-immunoprecipitated with NBPF1. PMID: 20096688
  13. These findings unravel the molecular basis through which a combinatorial action of Cby and 14-3-3 proteins controls the dynamic nuclear-cytoplasmic trafficking of beta-catenin. PMID: 19940019
  14. Chibby inhibits beta-catenin-mediated transcriptional activation by competing with Lef-1 to bind to beta-catenin PMID: 12712206
  15. PIGEA-14 plays an important role in regulating the intracellular location of polycystin-2 PMID: 15194699
  16. As no somatic mutation was detected in C22orf2 in 36 colorectal tumour DNA, our results do not support the implication of Chibby as a tumour suppressor in colorectal carcinogenesis. PMID: 15245581
  17. Chibby is not likely to promote colorectal carcinoma tumor development or progression. PMID: 16570344
  18. CBY promotes adipocyte differentiation through inhibition of beta catenin signaling. PMID: 17403895
  19. intrinsically disordered TC-1 interacts with Cby via its transient helical structure PMID: 17905836
  20. 14-3-3epsilon and 14-3-3zeta are identified as Cby-binding partners. PMID: 18573912
  21. genes including CHIBBY involved in pediatric ependymomas. PMID: 18663750
  22. Alanine substitutions of two or more of four critical leucine residues within the C-terminal heptad repeats eliminate the Cby-Cby interaction. PMID: 19435523

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Subcellular Location
Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus. Golgi apparatus, trans-Golgi network.
Protein Families
Chibby family
Tissue Specificity
Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors.
Database Links

HGNC: 1307

OMIM: 607757

KEGG: hsa:25776

STRING: 9606.ENSP00000216029

UniGene: Hs.334911

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