Recombinant Human Protein dpy-19 homolog 2 (DPY19L2), partial

Code CSB-YP757514HU
MSDS
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Source Yeast
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Code CSB-EP757514HU
MSDS
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Source E.coli
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Code CSB-EP757514HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP757514HU
MSDS
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Source Baculovirus
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Code CSB-MP757514HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DPY19L2
Uniprot No.
Alternative Names
DPY19L2; UNQ3127/PRO10284; Probable C-mannosyltransferase DPY19L2; EC 2.4.1.-; Dpy-19-like protein 2; Protein dpy-19 homolog 2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable C-mannosyltransferase that mediates C-mannosylation of tryptophan residues on target proteins. Required during spermatogenesis for sperm head elongation and acrosome formation.
Gene References into Functions
  1. Low PLCZ1 expression is associated with globozoospermia with DPY19L2 deletion. PMID: 29339016
  2. provide new evidence, on the one hand, for a severe lack of maturation of the NL, and on the other hand, for dramatic modifications in the location of chromatin-related NL partners in DPY19L2-deleted spermatozoa PMID: 28882431
  3. Patients presenting with a monomorphic teratozoospermia such as globozoospermia or macrospermia with more than 85% of the spermatozoa presenting this specific abnormality have been analyzed permitting to identify several key genes for spermatogenesis such as AURKC and DPY19L2. PMID: 27779748
  4. Among Tunisian patients with globozoospermia, 8 DPY19L2 haplotypes were found. 61.1% were homozygous for a DPY19L2 deletion. A new splice-site mutation at the junction exon-intron 16 [c.1579_1580+4delAGGTAAinsTCAT] was found in 1 patient. PMID: 26516168
  5. The DPY19L2 mutations are the major cause of globozoospermia. PMID: 23512994
  6. Analysis of public databases at the DPY19L2 locus paradoxically revealed that, in the general population, duplications were approximately three times as frequent as deletions. PMID: 23555282
  7. DPY19L2 is the major gene responsible for globozoospermia and enlarges the spectrum of possible mutations in the gene. PMID: 22653751
  8. identification of DPY19L2 deletions and point mutations in European patients shows that globozoospemia caused by a molecular defect of DPY19L2 can be expected in individuals from any ethnic background PMID: 22627659
  9. Patients with globozoospermia have a homozygous deletion of DPY19L2. PMID: 21397064
  10. The relocation of the gene DPY19L2 within a set of low copy repeats. PMID: 16526957

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Involvement in disease
Spermatogenic failure 9 (SPGF9)
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Dpy-19 family
Tissue Specificity
Widely expressed with high expression in testis. Not detectable in ejaculated sperm (at protein level).
Database Links

HGNC: 19414

OMIM: 613893

KEGG: hsa:283417

STRING: 9606.ENSP00000315988

UniGene: Hs.533644

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