Recombinant Human Protein phosphatase 1 regulatory subunit 16A (PPP1R16A)

Code CSB-YP839340HU
MSDS
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Source Yeast
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Code CSB-EP839340HU
MSDS
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Source E.coli
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Code CSB-EP839340HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839340HU
MSDS
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Source Baculovirus
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Code CSB-MP839340HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PPP1R16A
Uniprot No.
Alternative Names
2900084E10Rik; Likley ortholog of mouse myosin phosphatase targeting subunit 3; MGC14333; Myosin phosphatase target subunit 3; Myosin phosphatase targeting subunit 3 ; Myosin phosphatase-targeting subunit 3; MYPT3 ; PP16A_HUMAN; PPP1R16A; Protein phosphatase 1 regulatory subunit 16A; Protein phosphatase 1, regulatory (inhibitor) subunit 16A; R75527
Species
Homo sapiens (Human)
Expression Region
1-525
Target Protein Sequence
MAEHLELLAE MPMVGRMSTQ ERLKHAQKRR AQQVKMWAQA EKEAQGKKGP GERPRKEAAS QGLLKQVLFP PSVVLLEAAA RNDLEEVRQF LGSGVSPDLA NEDGLTALHQ CCIDDFREMV QQLLEAGANI NACDSECWTP LHAAATCGHL HLVELLIASG ANLLAVNTDG NMPYDLCDDE QTLDCLETAM ADRGITQDSI EAARAVPELR MLDDIRSRLQ AGADLHAPLD HGATLLHVAA ANGFSEAAAL LLEHRASLSA KDQDGWEPLH AAAYWGQVPL VELLVAHGAD LNAKSLMDET PLDVCGDEEV RAKLLELKHK HDALLRAQSR QRSLLRRRTS SAGSRGKVVR RVSLTQRTDL YRKQHAQEAI VWQQPPPTSP EPPEDNDDRQ TGAELRPPPP EEDNPEVVRP HNGRVGGSPV RHLYSKRLDR SVSYQLSPLD STTPHTLVHD KAHHTLADLK RQRAAAKLQR PPPEGPESPE TAEPGLPGDT VTPQPDCGFR AGGDPPLLKL TAPAVEAPVE RRPCC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Inhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates.
Gene References into Functions
  1. PPP1R16A gene expression is decreased in follicular variant of papillary thyroid carcinoma. PMID: 21509594
  2. analysis of a novel mechanism for the phosphorylation of MYPT3 by PKA and activation of the catalytic activity through direct interaction of a central region of MYPT3 with its N-terminal region PMID: 16920702
Subcellular Location
Cell membrane; Lipid-anchor.
Database Links

HGNC: 14941

OMIM: 609172

KEGG: hsa:84988

STRING: 9606.ENSP00000292539

UniGene: Hs.521937

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