Recombinant Human Protein phosphatase Slingshot homolog 1 (SSH1), partial

Code CSB-YP837881HU
MSDS
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Source Yeast
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Code CSB-EP837881HU
MSDS
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Source E.coli
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Code CSB-EP837881HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP837881HU
MSDS
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Source Baculovirus
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Code CSB-MP837881HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SSH1
Uniprot No.
Alternative Names
AW551225; Coro1c; coronin; actin binding protein 1C; Gm1394; Gm1395; hSSH-1L; mSSH 1L; OTTMUSP00000031415; Protein phosphatase Slingshot homolog 1; Slingshot 1; slingshot 1L; slingshot homolog 1; slingshot like 1; Slingshot1; SSH 1; SSH 1L; SSH-1L; SSH-like protein 1; Ssh1; SSH1_HUMAN
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
Gene References into Functions
  1. SSH1 binds to gelsolin via actin filaments in the cytosolic fraction. Gelsolin promoted solubilization of actin filaments and SSH1 in cell-free assays and in cultured cells. PMID: 25451266
  2. the N-terminal PH-like domain plays a critical role in F-actin binding and F-actin-mediated activation of the cofilin-phosphatase activity of SSH1 PMID: 27865840
  3. Results reveal that SSH1L is upregulated in a subset of PCs and that the SSH1L/cofilin-1 signal pathway is associated positively in PC with cell migration. PMID: 25684665
  4. Authors show that viral penetration-induced calcium release facilitated herpes simplex virus type 1 intracellular trafficking through activating slingshot 1 (SSH), a phosphatase regulating actin filament dynamics. PMID: 24670325
  5. The association of IRS4 with SSH1 contributes to localized activation of cofilin in membrane protrusions. PMID: 25100728
  6. Slingshot 1 downregulation in neuronal cells inactivates cofilin to facilitate HSV-1 replication. PMID: 24418541
  7. Results identify a novel frameshift mutation in this gene implicated in disseminated superficial actinic porokeratosis in 4 Chinese families PMID: 23834120
  8. Thus this study provides evidence for a novel role of LIMK1 and SSH-1L in selectively regulating endothelial cell inflammation associated with intravascular coagulation. PMID: 24039253
  9. Our work provides evidence that supports the positive role of SSH1L in the mechanism of stem cell differentiation into cardiomyocyte-like cells. PMID: 23247370
  10. A high expression of SSH-1L in peripheral eosinophils in acute exacerbations of asthma may play a role in the activation and migration of eosinophils. PMID: 21690039
  11. EphA signaling promotes actin-based dendritic spine remodeling through slingshot 1. PMID: 22282498
  12. identified Ser 402 as a new phosphorylation site within the catalytic domain of human slingshot 1 (SSH1). PMID: 21525957
  13. Results show the significance of SSH1L in estrogen-induced osteoblastogenic differentiation in favor of adipogenic differentiation from hMSCs. PMID: 21289500
  14. SSH1 plays a critical role in cytokinesis by dephosphorylating and reactivating cofilin in later stages of mitosis PMID: 12807904
  15. findings suggest that SSH1L is activated downstream of PI3K and plays a critical role in insulin-induced membrane protrusion by dephosphorylating and activating cofilin PMID: 14645219
  16. SSH1L is locally activated by translocation to and association with F-actin in lamellipodia in response to neuregulin-1beta and 14-3-3 proteins negatively regulate SSH1L activity by sequestering it in the cytoplasm. PMID: 15159416
  17. SSH1 mutations seen in patients with disseminated superficial actinic porokeratosis PMID: 15459975
  18. a multi-protein complex consisting of SSH-1L, LIMK1, actin, and the scaffolding protein, 14-3-3zeta, is involved, along with the kinase, PAK4, in the regulation of ADF/cofilin activity PMID: 15660133
  19. Ca2+-induced cofilin dephosphorylation is mediated by calcineurin-dependent activation of SSH1L PMID: 15671020
  20. identified sites that play unique roles in the control of subcellular localization and F-actin-mediated activation of SSH1L PMID: 16513117
  21. Expression of phosphatase-dead versions of SSH proteins, SSH1, SSH2 & SSH3 results in phosphorylation/inactivation of cofilin, changes in cytoskeleton organization, loss of cell polarity, and assembly of aberrant arrays of laminin-332 in keratinocytes. PMID: 17848544
  22. PDGF participates in actin dynamics by dual regulation of cofilin activity via LIMK and SSH1L in aortic smooth muscle cells PMID: 18096821
  23. the conserved SSH-N domain of slingshot-1 plays critical roles in P-cofilin recognition, F-actin-mediated activation, and subcellular localization of SSH1 PMID: 18809681
  24. Results suggest that the formation of ROS by NADPH oxidases engages a SSH-1L-cofilin pathway to regulate cytoskeletal organization and cell migration. PMID: 19339277
  25. These results implicate 14-3-3zeta/tau heterodimers as key regulators of SSH1 activity in keratinocytes and suggest they play a role in cytoskeleton remodeling during cell migration. PMID: 19371722
  26. Data identify PKD as a central regulator of the cofilin signaling network via direct phosphorylation and regulation of SSH1L. PMID: 19567672

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Subcellular Location
Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cleavage furrow. Midbody. Note=Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
Protein Families
Protein-tyrosine phosphatase family
Database Links

HGNC: 30579

OMIM: 606778

KEGG: hsa:54434

STRING: 9606.ENSP00000315713

UniGene: Hs.199763

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