Recombinant Human RNA-binding protein 8A (RBM8A)

Code CSB-YP896536HU
MSDS
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Source Yeast
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Code CSB-EP896536HU
MSDS
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Source E.coli
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Code CSB-EP896536HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896536HU
MSDS
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Source Baculovirus
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Code CSB-MP896536HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RBM8A
Uniprot No.
Alternative Names
Binder of OVCA1 1; Binder of OVCA1-1; BOV 1; BOV 1A; BOV 1B; BOV 1C; BOV-1; BOV1; BOV1A; BOV1B; BOV1C; HSPC 114; HSPC114; MDS 014; MDS014; RBM 8; RBM 8A; RBM 8B; RBM8; rbm8a; RBM8A_HUMAN; RBM8B; Ribonucleoprotein RBM 8; Ribonucleoprotein RBM 8A; Ribonucleoprotein RBM8; Ribonucleoprotein RBM8A; RNA binding motif protein 8; RNA binding motif protein 8A; RNA binding motif protein 8B; RNA binding protein 8A; RNA binding protein Y14; RNA-binding motif protein 8A; RNA-binding protein 8A; RNA-binding protein Y14; ZNRP; ZRNP 1; ZRNP1
Species
Homo sapiens (Human)
Expression Region
2-174
Target Protein Sequence
ADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
Gene References into Functions
  1. Findings demonstrate that Y14 nucleoplasmic localization is regulated not only by the previously reported N-terminal localization signal but also by the C-terminal serine/arginine repeat-containing region through phosphorylation and MAGOH binding to Y14. PMID: 29330450
  2. A direct link between Y14 and p53 expression and suggests a function for Y14 in DNA damage signaling. PMID: 28361991
  3. Results demonstrated that RBM8A is highly expressed in hepatocellular carcinoma (HCC), and patients with lower RBM8A expression level had longer overall survival and progression free survival. Also, results indicate that RBM8A promotes invasion and metastasis via epithelial-mesenchymal transition signaling pathway. PMID: 28259942
  4. Y14 may selectively and differentially modulate protein biosynthesis. PMID: 26887951
  5. this data suggests a novel role of RBM8a in the regulation of neurodevelopment. PMID: 26094033
  6. Mutations of RBM8A and TBX6 are associated with disorders of the mullerian ducts. PMID: 25813282
  7. It was demonstrated that Rbm8a is an essential neurogenesis regulator in embryonic cortical development. PMID: 25948253
  8. Depletion of RNA-binding protein RBM8A (Y14) causes cell cycle deficiency and apoptosis in human tumor cells. PMID: 23970407
  9. Y14 positively regulates signals for TNF-alpha-induced IL-6 production at multiple steps beyond an exon junction complex protein. PMID: 23817415
  10. Y14 mutants, which are deficient in binding to Magoh, could still be localized to the nucleus, suggesting the existence of both the nuclear import pathway and function for Y14 unaccompanied by Magoh PMID: 22355610
  11. Data show that Y14 interacts directly with the decapping factor Dcp2 and the 5' cap structure of mRNAs via different but overlapping domains. PMID: 23115303
  12. findings show that the two regulatory SNPs result in diminished RBM8A transcription in vitro and that Y14 expression is reduced in platelets from individuals with thrombocytopenia with absent radii (TAR) PMID: 22366785
  13. Y14 provides a regulatory link between pre-mRNA splicing and snRNP biogenesis. PMID: 21209085
  14. conserved physical linkage in medaka and human genomes PMID: 12054603
  15. Tethered function analysis shows that the Y14/hUpf3b interaction is essential for nonsense-mediated decay, while surprisingly the interaction between hUpf3b and hUpf2 is not. PMID: 12718880
  16. antagonistic post-translational modifications of Y14 may be involved in the remodeling of Y14-containing mRNPs PMID: 16100109
  17. The stable association of multiprotein exon junction complex core with RNA is maintained by inhibition of eIF4AIII ATPase activity by MAGOH-Y14. PMID: 16170325
  18. crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase eukaryotic initiation factor 4AIII bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic PMID: 16931718
  19. Increased TAP binding correlates with increased SF2/ASF binding, but not increased REF/Aly or Y14 binding. PMID: 18243119
  20. Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3. PMID: 18503751
  21. These results indicate that MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and Y14. PMID: 19254694

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Subcellular Location
Nucleus. Nucleus speckle. Cytoplasm.
Protein Families
RBM8A family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 9905

OMIM: 605313

KEGG: hsa:9939

STRING: 9606.ENSP00000333001

UniGene: Hs.591455

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