Product Details

Uniprot No.

Target Names

RBM8A

Alternative Names

Binder of OVCA1 1 antibody; Binder of OVCA1-1 antibody; BOV 1 antibody; BOV 1A antibody; BOV 1B antibody; BOV 1C antibody; BOV-1 antibody; BOV1 antibody; BOV1A antibody; BOV1B antibody; BOV1C antibody; HSPC 114 antibody; HSPC114 antibody; MDS 014 antibody; MDS014 antibody; RBM 8 antibody; RBM 8A antibody; RBM 8B antibody; RBM8 antibody; rbm8a antibody; RBM8A_HUMAN antibody; RBM8B antibody; Ribonucleoprotein RBM 8 antibody; Ribonucleoprotein RBM 8A antibody; Ribonucleoprotein RBM8 antibody; Ribonucleoprotein RBM8A antibody; RNA binding motif protein 8 antibody; RNA binding motif protein 8A antibody; RNA binding motif protein 8B antibody; RNA binding protein 8A antibody; RNA binding protein Y14 antibody; RNA-binding motif protein 8A antibody; RNA-binding protein 8A antibody; RNA-binding protein Y14 antibody; ZNRP antibody; ZRNP 1 antibody; ZRNP1 antibody

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide derived from human RBM8A

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RBM8A, also known as RNA-binding protein Y14, serves as a core component of the exon junction complex (EJC), a multiprotein assembly deposited on mRNAs during splicing. This protein plays essential roles in nonsense-mediated mRNA decay, mRNA export, and translational regulation, making it a significant target for researchers investigating RNA processing, quality control mechanisms, and developmental biology. Notably, mutations in RBM8A have been linked to thrombocytopenia-absent radius syndrome, underscoring its importance in both basic research and disease-related studies.

This recombinant monoclonal antibody, generated from clone 7F9 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, researchers can expect reliable lot-to-lot performance across long-term projects and multi-site collaborations, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Validation studies demonstrate robust performance across multiple applications. Western blot analysis detects a clean band at the predicted 20 kDa molecular weight in human cell lines including MCF-7, K562, Jurkat, and HeLa, as well as rat brain tissue, confirming cross-species reactivity and suitability for both cell culture and tissue-based experiments. Immunofluorescence staining in MCF-7 cells reveals clear subcellular localization patterns, while flow cytometry analysis shows distinct positive population shifts compared to isotype controls, providing flexibility for researchers employing diverse detection strategies.

This antibody supports investigations into mRNA surveillance pathways, splicing regulation, and EJC function, serving researchers working across cancer biology, developmental disorders, and fundamental RNA biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.

Gene References into Functions

Findings demonstrate that Y14 nucleoplasmic localization is regulated not only by the previously reported N-terminal localization signal but also by the C-terminal serine/arginine repeat-containing region through phosphorylation and MAGOH binding to Y14. PMID: 29330450
A direct link between Y14 and p53 expression and suggests a function for Y14 in DNA damage signaling. PMID: 28361991
Results demonstrated that RBM8A is highly expressed in hepatocellular carcinoma (HCC), and patients with lower RBM8A expression level had longer overall survival and progression free survival. Also, results indicate that RBM8A promotes invasion and metastasis via epithelial-mesenchymal transition signaling pathway. PMID: 28259942
Y14 may selectively and differentially modulate protein biosynthesis. PMID: 26887951
this data suggests a novel role of RBM8a in the regulation of neurodevelopment. PMID: 26094033
Mutations of RBM8A and TBX6 are associated with disorders of the mullerian ducts. PMID: 25813282
It was demonstrated that Rbm8a is an essential neurogenesis regulator in embryonic cortical development. PMID: 25948253
Depletion of RNA-binding protein RBM8A (Y14) causes cell cycle deficiency and apoptosis in human tumor cells. PMID: 23970407
Y14 positively regulates signals for TNF-alpha-induced IL-6 production at multiple steps beyond an exon junction complex protein. PMID: 23817415
Y14 mutants, which are deficient in binding to Magoh, could still be localized to the nucleus, suggesting the existence of both the nuclear import pathway and function for Y14 unaccompanied by Magoh PMID: 22355610
Data show that Y14 interacts directly with the decapping factor Dcp2 and the 5' cap structure of mRNAs via different but overlapping domains. PMID: 23115303
findings show that the two regulatory SNPs result in diminished RBM8A transcription in vitro and that Y14 expression is reduced in platelets from individuals with thrombocytopenia with absent radii (TAR) PMID: 22366785
Y14 provides a regulatory link between pre-mRNA splicing and snRNP biogenesis. PMID: 21209085
conserved physical linkage in medaka and human genomes PMID: 12054603
Tethered function analysis shows that the Y14/hUpf3b interaction is essential for nonsense-mediated decay, while surprisingly the interaction between hUpf3b and hUpf2 is not. PMID: 12718880
antagonistic post-translational modifications of Y14 may be involved in the remodeling of Y14-containing mRNPs PMID: 16100109
The stable association of multiprotein exon junction complex core with RNA is maintained by inhibition of eIF4AIII ATPase activity by MAGOH-Y14. PMID: 16170325
crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase eukaryotic initiation factor 4AIII bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic PMID: 16931718
Increased TAP binding correlates with increased SF2/ASF binding, but not increased REF/Aly or Y14 binding. PMID: 18243119
Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3. PMID: 18503751
These results indicate that MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and Y14. PMID: 19254694

Hide All

Subcellular Location

Nucleus. Nucleus speckle. Cytoplasm.

Protein Families

RBM8A family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 9905

OMIM: 605313

KEGG: hsa:9939

STRING: 9606.ENSP00000333001

UniGene: Hs.591455

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Code	CSB-RA171029A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate(30µg),K562 whole cell lysate(30µg),Jurkat whole cell lysate(30µg),Hela whole cell lysate(30µg), Rat brain tissue lysate(20µg) All lanes: RBM8A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time：1min Immunofluorescence staining of MCF-7 cell with CSB-RA171029A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF-7 cells stained with CSB-RA171029A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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RBM8A Recombinant Monoclonal Antibody

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