Recombinant Human Ras GTPase-activating protein nGAP (RASAL2), partial

Code CSB-YP890908HU
MSDS
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Source Yeast
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Code CSB-EP890908HU
MSDS
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Source E.coli
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Code CSB-EP890908HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890908HU
MSDS
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Source Baculovirus
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Code CSB-MP890908HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RASAL2
Uniprot No.
Alternative Names
nGAP; NGAP_HUMAN; Ras GTPase-activating protein nGAP; Ras protein activator like 1; RAS protein activator-like 2; RASAL2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibitory regulator of the Ras-cyclic AMP pathway.
Gene References into Functions
  1. Furthermore, overexpression of miR-203 exhibited a similar effect to RASAL2 knockdown in hepatocellular carcinoma (HCC) cells. These results indicated that RASAL2 serves a tumor oncogenic role in HCC and may be considered a potential target in HCC. PMID: 28447723
  2. Study shows that up to 62% of luminal B cancers have lost expression of at least one of the DAB2IP and RASAL2 genes. However, the tumors that have lost both genes frequently present as advanced disease and are more likely to recur. Importantly, the report provide evidence that DAB2IP and RASAL2 can individually function as tumor suppressors in breast cancer. PMID: 27974415
  3. RASAL2 could inhibit BCa tumorigenesis. PMID: 28182001
  4. these results demonstrated that miRNA-136 was a key anti-invasive miRNA and further confirmed the oncogenic role of RASAL2 in triple-negative breast cancer. PMID: 27108696
  5. High RASAL2 expression is associated with liver cancer. PMID: 24763612
  6. these results indicate that activation of a RASAL2/ARHGAP24/RAC1 module contributes to TNBC tumorigenesis and identify a context-dependent role of RASAL2 in breast cancer. PMID: 25384218
  7. Results implicate RASAL2 as an EMT regulator and tumor suppressor in ovarian cancer, and down-regulation of RASAL2 promotes ovarian cancer progression. PMID: 25216515
  8. the down-regulation of RASAL2 promotes metastatic progression of lung adenocarcinoma PMID: 25446096
  9. The RasGAP gene, RASAL2, is a tumor and metastasis suppressor. PMID: 24029233
  10. RASAL2 was identified as an ECT2-interacting protein that regulates RHO activity in astrocytoma cells. PMID: 22683310

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Database Links

HGNC: 9874

OMIM: 606136

KEGG: hsa:9462

STRING: 9606.ENSP00000356621

UniGene: Hs.496139

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