Product Details

Purity

≥ 95% as determined by SDS-PAGE.
≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human PTPRD protein at 2 μg/mL can bind Human IL1RAPL1 protein (CSB-MP011624HU1). The EC50 is 3.361-4.394 ng/mL.

Target Names

PTPRD

Uniprot No.

P23468

Alternative Names

Receptor-type tyrosine-protein phosphatase delta; EC:3.1.3.48; Protein-tyrosine phosphatase delta; R-PTP-delta; PTPRD

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

21-1265aa

Target Protein Sequence

ETPPRFTRTPVDQTGVSGGVASFICQATGDPRPKIVWNKKGKKVSNQRFEVIEFDDGSGSVLRIQPLRTPRDEAIYECVASNNVGEISVSTRLTVLREDQIPRGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDTSNNNGRIKQLRSESIGGTPIRGALQIEQSEESDQGKYECVATNSAGTRYSAPANLYVRELREVRRVPPRFSIPPTNHEIMPGGSVNITCVAVGSPMPYVKWMLGAEDLTPEDDMPIGRNVLELNDVRQSANYTCVAMSTLGVIEAIAQITVKALPKPPGTPVVTESTATSITLTWDSGNPEPVSYYIIQHKPKNSEELYKEIDGVATTRYSVAGLSPYSDYEFRVVAVNNIGRGPPSEPVLTQTSEQAPSSAPRDVQARMLSSTTILVQWKEPEEPNGQIQGYRVYYTMDPTQHVNNWMKHNVADSQITTIGNLVPQKTYSVKVLAFTSIGDGPLSSDIQVITQTGVPGQPLNFKAEPESETSILLSWTPPRSDTIANYELVYKDGEHGEEQRITIEPGTSYRLQGLKPNSLYYFRLAARSPQGLGASTAEISARTMQSKPSAPPQDISCTSPSSTSILVSWQPPPVEKQNGIITEYSIKYTAVDGEDDKPHEILGIPSDTTKYLLEQLEKWTEYRITVTAHTDVGPGPESLSVLIRTNEDVPSGPPRKVEVEAVNSTSVKVSWRSPVPNKQHGQIRGYQVHYVRMENGEPKGQPMLKDVMLADAQWEFDDTTEHDMIISGLQPETSYSLTVTAYTTKGDGARSKPKLVSTTGAVPGKPRLVINHTQMNTALIQWHPPVDTFGPLQGYRLKFGRKDMEPLTTLEFSEKEDHFTATDIHKGASYVFRLSARNKVGFGEEMVKEISIPEEVPTGFPQNLHSEGTTSTSVQLSWQPPVLAERNGIITKYTLLYRDINIPLLPMEQLIVPADTTMTLTGLKPDTTYDVKVRAHTSKGPGPYSPSVQFRTLPVDQVFAKNFHVKAVMKTSVLLSWEIPENYNSAMPFKILYDDGKMVEEVDGRATQKLIVNLKPEKSYSFVLTNRGNSAGGLQHRVTAKTAPDVLRTKPAFIGKTNLDGMITVQLPEVPANENIKGYYIIIVPLKKSRGKFIKPWESPDEMELDELLKEISRKRRSIRYGREVELKPYIAAHFDVLPTEFTLGDDKHYGGFTNKQLQSGQEYVFFVLAVMEHAESKMYATSPYSDPVVSMDLDPQPITDEEE

Mol. Weight

139.5 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

PTPRD regulates synaptic plasticity and neuronal connectivity through interactions with postsynaptic adhesion molecules, making it a critical target for investigating neurodevelopmental and psychiatric disorders. This mammalian-expressed construct spanning residues 21–1265 captures the extracellular and membrane-proximal domains essential for ligand recognition, and its binding to IL1RAPL1 has been quantitatively validated by functional ELISA with an EC50 of 3.361–4.394 ng/mL, providing a defined benchmark for receptor-ligand interaction assays and competitive inhibition studies. The demonstrated affinity supports use in therapeutic antibody epitope mapping, blocking antibody screening, and SPR- or BLI-based affinity characterization experiments where precise KD determination is required. Dual-method purity confirmation by SDS-PAGE and SEC-HPLC (both >95%) combined with endotoxin levels below 1.0 EU/μg aligns with standards expected in functional binding assays and small-molecule inhibitor screening campaigns targeting PTPRD-mediated signaling pathways.

Target Background

Function

Can bidirectionally induce pre- and post-synaptic differentiation of neurons by mediating interaction with IL1RAP and IL1RAPL1 trans-synaptically. Involved in pre-synaptic differentiation through interaction with SLITRK2.

Gene References into Functions

PTPRD rs35929428 might play a role in hepatic lipid accumulation and fibrosis, followed by the development of NAFLD. PMID: 28497593
Results show the existence of a perturbed PTPRD-STAT3 axis potentially driving malignant progression of Hepatitis C virus-associated liver disease. PMID: 28159835
Presynaptic differentiation induced by protein tyrosine phosphatase receptor type D (PTPdelta)-leucine rich repeat and fibronectin type III domain containing 5 (SALM5) requires the dimeric property of SALM5. PMID: 29348429
In the 2:2 heterotetrameric SALM5/PTPdelta complex, a SALM5 dimer bridges two separate PTPdelta molecules. PMID: 29348579
our study suggested that PTPRD loci are candidate susceptibility regions that have some marker SNPs for GDM in Han Chinese PMID: 27738328
We investigated the modification of air pollution and diabetes association by a genetic risk score covering 63 T2D genes. Five single variants near GRB14, UBE2E2, PTPRD, VPS26A and KCNQ1 showed nominally significant interactions with PM10 (P<0.05). Our results suggest that genetic risk for T2D may modify susceptibility to air pollution through alterations in insulin sensitivity PMID: 27281273
Mutation in PTPRD gene is associated with nodal marginal zone lymphoma. PMID: 27335277
Low PTPRD expression is associated with Head and Neck Squamous Cell Carcinoma. PMID: 27501229
The data provide evidence of the high incidence of PTPRD inactivation in laryngeal squamous cell carcinoma, suggesting that deletions and loss-of-function mutations are responsible for PTPRD loss only in a fraction of cases, whereas DNA methylation is the dominating mechanism of PTPRD inactivation. PMID: 28345455
PTPRD polymorphisms might modulate antipsychotic-induced weight gain. PMID: 26656879
overexpression in acute myeloid leukaemia cells inhibited cell proliferation and clonogenicity as well as inducing apoptosis PMID: 26607758
PTPRD was identified as a novel locus potentially associated with blood pressure response to atenolol and resistant hypertension in multiple ethnic groups. PMID: 26425837
PTPRD mutation, but not methylation or copy number loss, may serve as a predictive biomarker of sensitivity to STAT3 inhibitors in HNSCC. PMID: 26267899
Study shows for the first time that DNMT1 caused PTPRD DNA hypermethylation and induced insulin signaling silencing in T2D patients. PMID: 26079428
PTPRD is homozygously deleted and epigenetically downregulated in hepatocellular carcinomas (HCCs). PMID: 25831062
This study demonstrated that the copy number variations of PTPRD relate to opioid dependence. PMID: 25345593
Silencing PTPRD expression by siRNA treatment significantly enhanced cell proliferation compared with mock siRNA treatment. PMID: 25412184
we report desmoplakin, a desmosomal protein that is implicated in cell-cell adhesion, as a novel PTPRD substrate. PMID: 25113440
Ptprd is a tumor suppressor that can promote tumorigenesis in concert with Cdkn2a loss. PMID: 25138050
The absence of MIM led to PTPdelta-mediated activation of SRC. PMID: 25287652
We reveal that PTPRD is a bona fide tumor suppressor, pinpoint PTPRD loss as a cause of aberrant STAT3 activation in gliomas, and establish PTPRD loss, in the setting of CDKN2A/p16(INK4A) deletion, as a driver of glioma progression. PMID: 24843164
Data suggest that PTPRD germline mutations may play a role in the development of Ewing sarcoma. PMID: 23800680
Data indicate that expression of several predicted chimeric genes and genes with disrupted exon structure including ALK, NBAS, FHIT, PTPRD and ODZ4 in neuroblastoma. PMID: 23991058
Loss of PTPRD gene is associated with squamous cell carcinomas of the vulva. PMID: 23404381
Polymorphism in protein tyrosine phosphatase receptor delta is associated with the risk of clear cell renal cell carcinoma. PMID: 23069849
This is the first report to observe an association between deletion within PTPRD and metastatic cutaneous squamous cell carcinoma. PMID: 22052591
Although endogenous PTPdelta expression was very low in neuroblastoma cells, it was also low in mouse embryo adrenal glands, suggesting that PTPdelta may have little developmental function in early adrenal neuroblasts. PMID: 22571343
Data indicate that genetic variants near the KCNQ1 and MAF/WWOX genes are associated with reduced insulin secretion, and the PTPRD genetic variant appears to be associated with progression to diabetes in Han Chinese. PMID: 21767287
PTPRD has a tumor suppressor function in neuroblastoma through AURKA dephosphorylation and destabilization and a downstream destabilization of MYCN protein, representing a novel mechanism for the function of PTPRD in neuroblastoma. PMID: 22305495
The interaction of the IL1RAPL1 family of proteins with PTPdelta and RhoGAP2 reveals a pathophysiological mechanism of cognitive impairment associated with a novel type of trans-synaptic signaling. PMID: 21926414
Eight suggestive significant loci were detected with a series of genes expressed within the inner ear that underlie the auditory function, such as: DCLK1, PTPRD, GRM8, CMIP. PMID: 21493956
Both family-based and population-based association studies suggest that PTPRD variant rs1975197 confers risk of RLS. PMID: 21264940
statistical significant downregulation of PCDH17/PCH68 and PTPRD was observed PMID: 21213369
Results found the PTPRD gene was most frequently homozygously deleted gene in human lung cancer among genes mapping to regions other than 9p21. PMID: 20073072
Trans-synaptic adhesions between netrin-G ligand-3 (NGL-3) and receptor tyrosine phosphatases LAR, protein-tyrosine phosphatase delta (PTPdelta), and PTPsigma via specific domains regulate excitatory synapse formation. PMID: 20139422
A novel isoform of PTPRD was cloned and its expression analyzed. PMID: 16114034
data identify PTPRD as a candidate tumor suppressor gene in cutaneous squamous cell carcinoma with a possible association with metastasis PMID: 17420988
The aberrant splicing or microdeletion of 5' UTR exons in such a high proportion of tumors indicates that loss of these exons dys-regulates the mRNA sequence PMID: 18050303
The results indicate that polymorphisms of PTPRD are strongly associated with pediatric bronchial asthma in the Taiwanese population. PMID: 18414509
Two independent SNPs in the 5' UTR of splice variants expressed predominantly in the central nervous system showed highly significant P values, identifying PTPRD as the fourth genome-wide significant locus for restless legs syndrome. PMID: 18660810
These results implicate PTPRD as a tumor suppressor on chromosome 9p that is involved in the development of glioblastoma multiforme and multiple human cancers. PMID: 19478061
No mutations of PTPRD were associated with familiar melanoma PMID: 19500277
Identification of ZNF366 and PTPRD as novel determinants of plasma homocysteine in a family-based genome-wide association study PMID: 19525478

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Protein-tyrosine phosphatase family, Receptor class 2A subfamily

Database Links

HGNC: 9668

OMIM: 601598

KEGG: hsa:5789

STRING: 9606.ENSP00000348812

UniGene: Hs.446083

Code	CSB-MP019051HU(A4)
Abbreviation	Recombinant Human PTPRD protein, partial (Active)
MSDS	MSDS Datasheet
Size	$190
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human PTPRD protein at 2 μg/ml can bind Human IL1RAPL1 protein (CSB-MP011624HU1). The EC₅₀ is 3.361-4.394 ng/mL. Biological Activity Assay The purity of PTPRD was greater than 95% as determined by SEC-HPLC
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Recombinant Human Receptor-type tyrosine-protein phosphatase delta (PTPRD), partial (Active)

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Target Background

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