Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

GPRC5A

Uniprot No.

Q8NFJ5

Research Area

Neuroscience

Alternative Names

GPRC5A; GPCR5A; RAI3; RAIG1; Retinoic acid-induced protein 3; G-protein coupled receptor family C group 5 member A; Phorbol ester induced gene 1; PEIG-1; Retinoic acid-induced gene 1 protein; RAIG-1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

269-357aa

Target Protein Sequence

TKQRNPMDYPVEDAFCKPQLVKKSYGVENRAYSQEEITQGFEETGDTLYAPYSTHFQLQNQPPQKEFSIPRAHAWPSPYKDYEVKKEGS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

37.0 kDa

Protein Length

Partial

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 269-357 form the expressed segment for recombinant Human GPRC5A. The theoretical molecular weight of the GPRC5A protein is 37.0 kDa. This GPRC5A protein is produced using e.coli expression system. The GPRC5A gene fragment has been modified by fusing the N-terminal GST tag, providing convenience in detecting and purifying the recombinant GPRC5A protein during the following stages.

Retinoic acid-induced protein 3 (RAI3), also known as GPRC5A, belongs to the G protein-coupled receptor (GPCR) family. GPRC5A is expressed in several tissues, including the lung, and has been implicated in processes such as cell proliferation, differentiation, and apoptosis. GPRC5A's role in cancer biology, particularly in lung cancer, is of significant interest, as it may function as a tumor suppressor or promoter depending on the context.

Target Background

Function

Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling. May act as a lung tumor suppressor.

Gene References into Functions

GPRC5a was upregulated in pancreatic cancer (PaCa) leading to an enhanced drug resistance in PaCa cells. PMID: 29949874
under-expressed GPRC5A during lung tumorigenesis enhances any transcriptional stimulation through an active translational status. PMID: 27273304
findings reveal an unprecedented role for GPRC5A in regulation of the ITGB1-mediated cell adhesion and it's downstream signaling, thus indicating a potential novel role for GPRC5A in human epithelial cancers. PMID: 27715394
miR-204 inhibits cell proliferation in gastric cancer by targeting CKS1B, CXCL1 and GPRC5A. PMID: 29283424
p53 overexpression and GPRC5A induction markedly inhibited tumor cell viability and induced apoptosis. PMID: 28849235
GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR. PMID: 27415424
These results suggest RAI3 plays an important role in adipogenesis of hASCs and may have a potential use in the future application. PMID: 28870805
Study underscores genomic alterations that represent early events in the development of Kras mutant LUAD following Gprc5a loss and tobacco carcinogen exposure. PMID: 28653505
Suppression of GPRC5a results in decreased cell growth, proliferation and migration in pancreatic cancer cell lines via a STAT3 modulated pathway, independent from ERK activation PMID: 28114355
our results implicate GPRC5A as a tumor suppressor in breast cancer cells, and GPRC5A exerts its tumor-suppressive function by inhibiting EGFR and its downstream pathway PMID: 27599526
elevated levels of GPRC5A played significant roles in gastric cancer progression PMID: 26227221
Results show how GPRC5A deficiency leads to dysregulated EGFR and STAT3 signaling and lung tumorigenesis. PMID: 25744720
EGFR interacted with GPRC5A and phosphorylated it in two conserved double-tyrosine motifs, Y317/Y320 and Y347/ Y350, at the C-terminal tail of GPRC5A. PMID: 25311788
The interaction of miR-103a-3p with each of the two 5' UTR targets reduces the expression levels of both GPRC5A mRNA and GPRC5A protein in one normal epithelial and two pancreatic cancer cell lines. PMID: 24984703
RAI3 may contribute to the malignant progression of hepatocellular carcinoma PMID: 23632812
Data indicate that in mammary tumors, the mRNA expression of GPRC5A significantly correlated with that of BRCA1. PMID: 24470238
Decreased GPRC5A expression is associated with non-small cell lung cancers and lung inflammation. PMID: 23154545
Loss of GPRC5A is associated with lung adenocarcinomas. PMID: 20563252
RAI3 is a cell growth-promoting gene and a novel P53 transcriptional target PMID: 15659406
This evidence explains one of the mechanisms of the GPRC5A-regulated cell growth in some cancer cell lines. PMID: 17055459
Gprc5a functions as a tumor suppressor in mouse lung, and human GPRC5A may share this property. PMID: 18000218
Mechanisms underlying the induction of the putative human tumor suppressor GPRC5A are reported. PMID: 19279407
analysis of RAI3 expression in normal and cancerous human breast tissue at both the mRNA and protein levels PMID: 19552806

Hide All

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 3 family

Tissue Specificity

Expressed at high level in fetal and adult lung tissues but repressed in most human lung cancers. Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at low to mod

Database Links

HGNC: 9836

OMIM: 604138

KEGG: hsa:9052

STRING: 9606.ENSP00000014914

UniGene: Hs.631733

Code	CSB-EP818781HU1
Abbreviation	Recombinant Human GPRC5A protein, partial
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Retinoic acid-induced protein 3 (GPRC5A), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage