The recombinant human Ribonuclease T2 (RNASET2) protein is produced by constructing the recombinant plasmid encoding the human RNASET2 protein (25-256aa) along with the C-terminal hFc-tag, transforming recombinant plasmid into mammalian cells, screening the positive mammalian cells and culturing them, and inducing the protein expression. Following expression, the recombinant RNASET2 protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant human RNASET2 protein. Its purity is greater than 90%.
RNASET2, part of the T2 ribonuclease family, is a versatile protein with many roles in biology. Besides its traditional job of breaking down RNA, recent studies show it has other functions too [1][2]. It's important for the immune system, affecting how different immune cells work [3][4]. In cancer, RNASET2 can inhibit tumor formation like ovarian cancer or promote tumor growth [5][6]. In diseases like Crohn's disease, it seems to have a mixed effect on inflammation [7]. It also influences fat production and cell movement in kidney cancer, showing its involvement in different diseases [8].
RNASET2 is connected to various health problems, from cancer to infertility and nerve damage, showing it's vital for many body functions [9]. It helps control cell growth and can slow down the growth of tumors [10][11]. In ovarian cancer, it seems to have a role in controlling which genes are active, which affects how cancer develops [12].
References:
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[2] S. Monaci, F. Coppola, G. Giuntini, R. Roncoroni, F. Acquati, S. Sozzaniet al., Hypoxia enhances the expression of rnaset2 in human monocyte-derived dendritic cells: role of pi3k/akt pathway, International Journal of Molecular Sciences, vol. 22, no. 14, p. 7564, 2021. https://doi.org/10.3390/ijms22147564
[3] F. Acquati, L. Mortara, A. Vito, D. Baci, A. Albini, M. Cippitelliet al., Innate immune response regulation by the human rnaset2 tumor suppressor gene, Frontiers in Immunology, vol. 10, 2019. https://doi.org/10.3389/fimmu.2019.02587
[4] N. Baranzini, L. Monti, M. Vanotti, V. Orlandi, F. Bolognese, D. Scaldaferriet al., Aif-1 and rnaset2 play complementary roles in the innate immune response of medicinal leech, Journal of Innate Immunity, vol. 11, no. 2, p. 150-167, 2018. https://doi.org/10.1159/000493804
[5] M. Ji, Z. Zhao, Y. Li, P. Xu, J. Shi, Z. Liet al., Fbxo6-mediated rnaset2 ubiquitination and degradation governs the development of ovarian cancer, Cell Death and Disease, vol. 12, no. 4, 2021. https://doi.org/10.1038/s41419-021-03580-4
[6] F. Acquati, M. Lualdi, S. Bertilaccio, L. Monti, G. Turconi, M. Fabbriet al., Loss of function of ribonuclease t2, an ancient and phylogenetically conserved rnase, plays a crucial role in ovarian tumorigenesis, Proceedings of the National Academy of Sciences, vol. 110, no. 20, p. 8140-8145, 2013. https://doi.org/10.1073/pnas.1222079110
[7] E. Biener-Ramanujan, F. Rosier, S. Coetzee, D. McGovern, D. Hazelett, S. Targanet al., Diagnostic and therapeutic potential of rnaset2 in crohn’s disease: disease-risk polymorphism modulates allelic-imbalance in expression and circulating protein levels and recombinant-rnaset2 attenuates pro-inflammatory cytokine secretion, Frontiers in Immunology, vol. 13, 2022. https://doi.org/10.3389/fimmu.2022.999155
[8] Y. Quan, J. Dai, S. Zhou, L. Jin, Y. Long, S. Liuet al., hif2α‐induced upregulation of rnaset2 promotes triglyceride synthesis and enhances cell migration in clear cell renal cell carcinoma, Febs Open Bio, vol. 13, no. 4, p. 638-654, 2023. https://doi.org/10.1002/2211-5463.13570
[9] G. Caputa, S. Zhao, A. Criado, D. Ory, J. Duncan, & J. Schaffer, Rnaset2 is required for ros propagation during oxidative stress-mediated cell death, Cell Death and Differentiation, vol. 23, no. 2, p. 347-357, 2015. https://doi.org/10.1038/cdd.2015.105
[10] F. Yin, L. Liu, X. Liu, Y. Chen, L. Zheng, D. Liet al., Downregulation of tumor suppressor gene ribonuclease t2 and gametogenetin binding protein 2 is associated with drug resistance in ovarian cancer, Oncology Reports, vol. 32, no. 1, p. 362-372, 2014. https://doi.org/10.3892/or.2014.3175
[11] Q. Wang, X. Wang, & L. Xiang, Role and mechanism of rnaset2 in the pathogenesis of vitiligo, Journal of Investigative Dermatology Symposium Proceedings, vol. 17, no. 1, p. 48-50, 2015. https://doi.org/10.1038/jidsymp.2015.24
[12] G. Turconi, D. Scaldaferri, M. Fabbri, L. Monti, M. Lualdi, E. Pedriniet al., Rnaset2 silencing affects mirnas and target gene expression pattern in a human ovarian cancer cell model, International Journal of Oncology, vol. 49, no. 6, p. 2637-2646, 2016. https://doi.org/10.3892/ijo.2016.3763
