Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

RNH1

Uniprot No.

P13489

Research Area

Cardiovascular

Alternative Names

Placental ribonuclease inhibitor;Placental RNase inhibitor;Ribonuclease/angiogenin inhibitor 1;RAI

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-461aa

Target Protein Sequence

SLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGDVGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDPQCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCGVTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVLRAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISNNRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVESVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

57.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Dive into the world of cardiovascular research with our Recombinant Human RNH1 protein, a key component for understanding the function and regulation of ribonucleases. As a ribonuclease inhibitor (Placental ribonuclease inhibitor; Placental RNase inhibitor), RNH1 is crucial for the inhibition of ribonucleases, preserving RNA integrity and regulating various cellular processes. Its role in modulating ribonuclease activities makes it a significant protein in the field of cardiovascular research.

Our Recombinant Human RNH1 protein is expressed in E.coli, providing a full length of mature protein (2-461aa) with native folding and functionality. N-terminal 10xHis-tagged and C-terminal Myc-tagged, this protein allows for efficient purification and detection. With a purity greater than 85% as determined by SDS-PAGE, our Recombinant Human RNH1 protein ensures reliable and consistent results in your research projects. Available in both liquid and lyophilized powder forms, our Recombinant Human RNH1 protein is the perfect choice for advancing your cardiovascular investigations.

Target Background

Function

Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.

Gene References into Functions

Results show that a low expression of RNH1 in metastasis of bladder cancer and suggest that it might regulate the function of ILK through mutual binding. PMID: 27576342
a novel mechanism of ANG in regulating PI3K/AKT/mTOR signaling pathway via RI, is reported. PMID: 25193113
RNH1 bound to RNase 7 and suppressed its antimicrobial activity by blocking its ability to bind the cell wall of uropathogenic bacteria. PMID: 24107847
angiogenin and ILK signaling pathway plays a pivotal role in mediating the inhibitory effects of RI on melanoma cells growth PMID: 24769129
RI could play a novel role in inhibiting metastasis of bladder cancer through regulating EMT and ILK signaling pathways. PMID: 24768914
RNH1 as a regulator of HDACi resistance in gastric cancer PMID: 23584480
RI might play a novel role in the development of bladder cancer through regulating EMT and the ILK signaling pathway. PMID: 23703635
the anti-tumor effect of RNH1 is also involved in suppressing growth and metastasis PMID: 21125316
RNH1 (as a recombinant fusion protein) is localized to mitochondria, nuclei, and cytosol in HeLa and HCT cells; RNH1 appears to bind to various mitochondrial proteins. PMID: 21276451
Human ribonuclease inhibitor gene has antiangiogenic and antitumor effects when expressed in hematopoietic cells in mouse melanoma models. PMID: 15592448
The conformational and oxidative stabilities of both RIs increase upon complex formation with ribonucleases. Thus, RI has evolved to maintain its inhibition of invading ribonucleases, even when confronted with extreme environmental stress. PMID: 17956129
This the first measurement of the inhibition of the ribonucleolytic activity of onconase by ribonuclease inhibitor protein. PMID: 18930025

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Subcellular Location

Cytoplasm.

Database Links

HGNC: 10074

OMIM: 173320

KEGG: hsa:6050

STRING: 9606.ENSP00000346402

UniGene: Hs.530687

Code	CSB-EP019901HUb1
Abbreviation	Recombinant Human RNH1 protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Ribonuclease inhibitor (RNH1)

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