Recombinant Human SUN domain-containing protein 1 (SUN1), partial

Code CSB-YP529741HU
MSDS
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Source Yeast
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Code CSB-EP529741HU
MSDS
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Source E.coli
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Code CSB-EP529741HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP529741HU
MSDS
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Source Baculovirus
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Code CSB-MP529741HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SUN1
Uniprot No.
Alternative Names
Protein unc-84 homolog A; Sad1 and UNC84 domain containing 1; Sad1/unc 84 protein like 1; Sad1/unc-84 protein-like 1; Sun domain-containing protein 1; Sun1; SUN1_HUMAN; UNC 84 homolog A (C elegans); UNC 84A; UNC84 A; UNC84; UNC84 homolog A (C elegans); UNC84A
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Involved in telomere attachment to nuclear envelope in the prophase of meiosis implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly. Required for gametogenesis and involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs). Required for efficient localization of SYNE4 in the nuclear envelope. May be involved in nuclear remodeling during sperm head formation in spermatogenenis. May play a role in DNA repair by suppressing non-homologous end joining repair to facilitate the repair of DNA cross-links.
Gene References into Functions
  1. While Sun1 and Sun2 in HeLa cells are each able to bind KASH-domains, Sun1 is more efficiently incorporated into LINC complexes under normal growth conditions. Furthermore, the balance of Sun1 and Sun2 incorporated into LINC complexes is cell type-specific and is correlated with SRF/Mkl1-dependent gene expression. PMID: 29813079
  2. Low SUN1 expression is associated with HIV infections. PMID: 29643244
  3. Thus, AID is a conserved functional domain in SUN proteins and this work provides the structural evidence to support the conversation of the AID-mediated autoinhibition of SUN proteins. PMID: 29408528
  4. SUN1/SUN2 may function redundantly in early HIV-1 infection steps and therefore influence HIV-1 replication and pathogenesis. PMID: 28747499
  5. wndchrm revealed a consistent negative correlation between SUN1 expression and the size of nucleoli in human breast cancer tissues. PMID: 26962703
  6. Nuclear envelope associated endosome-mediated transfer depends on the nuclear envelope proteins SUN1 and SUN2, as well as the Sec61 translocon complex. PMID: 26356418
  7. SUN1 plays a role in hnRNP-involved mRNA export. PMID: 26476453
  8. An important role for SUN1 and SUN2 in muscle disease pathogenesis. PMID: 25210889
  9. these data support a model whereby mitotic phosphorylation of SUN1 disrupts interactions with nucleoplasmic binding partners, promoting disassembly of the nuclear lamina and, potentially, its chromatin interactions PMID: 25482198
  10. The Caenorhabditis elegans lamin, LMN-1, is required for nuclear migration and interacts with the nucleoplasmic domain of the SUN protein UNC-84. PMID: 25057012
  11. Results highlight the interactions at the nuclear envelope where mutations in the EMD and TMPO gene in combination with mutations in SUN1 have an impact on several components of the network. PMID: 24375709
  12. Farnesylation of progerin enhances its interaction with SUN1 and reduces SUN1 mobility. PMID: 24522183
  13. Codepletion of SUN1/2 slows cell proliferation and results in an accumulation of morphologically defective and disoriented mitotic spindles. PMID: 24662567
  14. Reduction of SUN1 overaccumulation in LMNA mutant fibroblasts and in cells derived from HGPS patients corrected nuclear defects and cellular senescence. PMID: 22541428
  15. These results demonstrate that the interplay between SUN1 and farnesylated prelamin A contributes to nuclear positioning in human myofibers and may be implicated in pathogenetic mechanisms. PMID: 21311568
  16. POM121 and Sun1 interact transiently to promote early steps of interphase nuclear pore complexes assembly PMID: 21727197
  17. perturbations in lamin A-SUN1 and SUN2 protein interactions may underlie the opposing effects of EDMD and HGPS mutations on nuclear and cellular mechanics PMID: 19933576
  18. Describes cloning and function of C. elegans unc-84 and cloning of human orthologs. PMID: 10375507
  19. The Sun1 itself does not require functional A-type lamins for its localisation at the inner nuclear membrane in mammalian cells. PMID: 16079285
  20. localization and anchoring of UNC84A is not dependent on the lamin proteins, in contrast to what had been observed for C. elegans UNC-84 PMID: 16445915
  21. SUN1 and SUN2 may form a physical interaction between the nuclear envelope and the centrosome PMID: 17132086
  22. chromosome de-condensation needs the function of an inner nuclear membrane (INM) protein hsSUN1 and a membrane-associated histone acetyltransferase (HAT), hALP. PMID: 17631499
  23. Results suggest that Sun1 represents an important determinant of nuclear pore complex distribution across the nuclear surface. PMID: 17724119
  24. Isolation of a cDNA encoding a mouse homolog of the human SUN1 (UNC84A) gene. PMID: 12036294
  25. KIAA0810 is a novel 100-kDa transmembrane protein with similarity to Caenorhabditis elegans Unc-84A and resides in the inner nuclear membrane. It is likely to interact with the nuclear lamina. PMID: 11593002

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Subcellular Location
Nucleus inner membrane; Single-pass type II membrane protein.
Database Links

HGNC: 18587

OMIM: 607723

KEGG: hsa:23353

STRING: 9606.ENSP00000384015

UniGene: Hs.438072

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