Recombinant Human Sciellin (SCEL)

Code CSB-YP020805HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP020805HU
MSDS
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Source E.coli
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Code CSB-EP020805HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020805HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP020805HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SCEL
Uniprot No.
Alternative Names
FLJ21667; MGC22531; Scel; SCEL_HUMAN; Sciellin
Species
Homo sapiens (Human)
Expression Region
1-688
Target Protein Sequence
MSNVTLRKMS PTGNEMKSTT QGTTRKQQDF HEVNKRRTFL QDNSWIKKRP EEEKDENYGR VVLNRHNSHD ALDRKVNERD VPKATISRYS SDDTLDRISD RNDAAKTYKA NTLDNQLTNR SMSMFRSLEV TKLQPGGSLN ANTSNTIAST SATTPVKKKR QSWFPPPPPG YNASSSTGTR RREPGVHPPI PPKPSSPVSS PNQLRQDNRQ IHPPKPGVYT ETNRSAERNI RSQDLDNIVK VATSLQRSDK GEELDNLIKM NKSLNRNQGL DSLFRANPKV EEREKRAKSL ESLIYMSTRT DKDGKGIQSL GSPIKVNQRT DKNEKGRQNL ESVAKVNARM NKTSRRSEDL DNATEVNPKG HENTTGKKDL DGLIKVDPET NKNITRGQSL DNLIKVTPEV KRSNQGSKDL NNFIKVYPGT EKSTEGGQSL DSLIKVTPER NRTNQGNQDL ENLIKVIPSA NKSSEQGLDE HINVSPKAVK NTDGKQDLDK LIKVNPEIFT NNQRNQDLAN LIKVNPAVIR NNQSQDLDNL IKVKPSALRN TNRDQNLENL IEVNSHVSEN KNGSSNTGAK QAGPQDTVVY TRTYVENSKS PKDGYQENIS GKYIQTVYST SDRSVIERDM CTYCRKPLGV ETKMILDELQ ICCHSTCFKC EICKQPLENL QAGDSIWIYR QTIHCEPCYS KIMAKWIP
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May function in the assembly or regulation of proteins in the cornified envelope. The LIM domain may be involved in homotypic or heterotypic associations and may function to localize sciellin to the cornified envelope.
Gene References into Functions
  1. These results demonstrate that SCEL is a mesenchymal-to-epithelial transition inducer dynamically regulated through the metastasis process PMID: 27013588
  2. Sciellin, SPRR3, and periplakin transcripts were all upregulated (4.67-, 4.95-, 2.77-fold, respectively) by prolonged exposure to cyclic strain (24-72 h), but not at earlier time points. PMID: 19211270
  3. VDUP1 and sciellin were more highly expressed in keratinocytes undergoing differentiation and colocalization of the proteins could be demonstrated PMID: 14632196
  4. Single nucleotide polymorphisms were detected in both matching tumor and normal esophageal tissues but no disease-associated mutations suggesting that SCEL is not a major factor in esophageal squamous cell carcinogenesis. PMID: 15274303
Subcellular Location
Cytoplasm. Membrane. Note=May become cross-linked to membrane proteins by transglutaminase.
Tissue Specificity
Highly expressed in esophagus. It is also expressed in keratinocytes, amniotic tissue, foreskin stratum spinosum and stratum granulosum, hair follicle and nail.
Database Links

HGNC: 10573

OMIM: 604112

KEGG: hsa:8796

STRING: 9606.ENSP00000302579

UniGene: Hs.534699

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