Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

SEMA7A

Uniprot No.

O75326

Research Area

Cardiovascular

Alternative Names

CD 108; CD108; CD108 antigen; CDw108; H Sema K1; H Sema L; H-SEMA-K1; H-Sema-L; JMH; JMH blood group antigen; John Milton Hagen blood group H Sema K1; John Milton Hargen human blood group Ag; John-Milton-Hargen human blood group Ag; MGC126692; MGC126696; SEM7A_HUMAN; Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A; Sema K1; Sema L; SEMA7A; SEMAK1; SEMAL; Semaphorin 7A GPI membrane anchor; Semaphorin K1; Semaphorin L; Semaphorin-7A; Semaphorin-K1; Semaphorin-L; Semaphorin7a

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

45-648aa

Target Protein Sequence

QGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

70.4kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The first step of making this recombinant Human SEMA7A protein is synthesizing the SEMA7A gene, the synthesized gene is then cloned into an expression vector which is a cDNA plasmid that includes a promoter sequence and an antibiotic-resistant gene. It also has N-terminal 6xHis tag encoding a fusion tag for downstream protein purification or identification. The antibiotic-resistant gene enables the selection of cells carrying the plasmid in antibiotic-based media and then transfects cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein. The recombinant SEMA7A protein is purified by ion-exchange chromatography or affinity purification. And the purity is 90%+ by SDS-PAGE.

SEMA7A plays a role in both innate and adaptive immune systems. It is a strong stimulator of monocytes in innate immunity, inducing the generation of pro-inflammatory cytokines. While SEMA7A works as a regulator of the T-cell immune response in adaptive immunity due to its effects on T-cell proliferation. SEMA7A can regulate T cell responses via the α1β1 integrin receptor. SEMA7A is a glycosylphosphatidylinositol (GPI)-anchored membrane protein with chemoattractant and chemorepulsive attributes. In addition to its role in guiding axon pathfinding during neuronal development, SEMA7A has diverse functions in morphogenesis and immune cell control.

Target Background

Function

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.

Gene References into Functions

These findings indicate that Sema7A as a potent activator of T cells and monocytes in the immune response contributes to the inflammation and progression of rheumatoid arthritis PMID: 28109308
short hairpin-mediated silencing of SEMA7A reveals roles for semaphorin 7a in the promotion of DCIS growth, motility and invasion as well as lymphangiogenesis in the tumor microenvironment. Our studies also uncover a relationship between COX-2 and semaphorin 7a expression and suggest that semaphorin 7a promotes tumor cell invasion on collagen and lymphangiogenesis via activation of b1-integrin receptor PMID: 27065336
Our results identified FGL2, GAL, SEMA4D, SEMA7A, and IDO1 as new candidate genes that could be involved in MSCs-mediated immunomodulation. FGL2, GAL, SEMA4D, SEMA7A, and IDO1 genes appeared to be differentially transcribed in the different MSC populations. Moreover, these genes were not similarly modulated following MSCs-exposure to inflammatory signals PMID: 28336906
Sema3A and sema7A expression correlated with the inflammatory activity of the multiple sclerosis (MS) lesions, suggesting their involvement in the immunological process that takes place in MS. PMID: 26432853
SEMA7A has a role in the development of lung injury PMID: 26752048
This study demonstrates that Sema7A controls the assembly of actin-based protrusions that drive Dendritic cell migration in response to CCL21. PMID: 26597008
These results suggest that SEMA7a plays a role as a CSF biomarkers associated with the conversion to clinically definite multiple sclerosis in clinically isolated syndromes patients PMID: 25406498
heterozygous missense variants in SEMA3A and SEMA7A may modify the phenotype of Kallmann syndrome but most likely are not alone sufficient to cause the disorder PMID: 24522099
SEMA7A was expressed in the liver and was increased in the course of liver fibrosis, both in mice and in humans. PMID: 23850082
Semaphorin 7A is a potentially important modulator of eosinophil profibrotic functions in the airway remodeling of patients with chronic asthma. PMID: 24333536
two MTRAP monomers interact via their tandem TSR domains with the Sema domains of a Semaphorin-7A homodimer PMID: 23166499
Semaphorin 7A protein variants differentially regulate T-cell activity. PMID: 22845496
Sema7A markedly reduces the production rates of megakaryocytes and platelets from CD34(+) progenitor cells. PMID: 22448926
We report here the expression and induction of semaphorin 7A (SEMA7A) on endothelium through hypoxia-inducible factor 1alpha during hypoxia. PMID: 22891341
Sema7A on keratinocytes and beta1-integrin on monocytes contribute to monocyte activation by keratinocytes within skin inflammation, inducing IL-8 PMID: 21524887
A new SEMA7A variant was identified in Native American plasma samples, and an alloantibody that recognizes the wild-type protein PMID: 20854351
Study reports the structures of Sema7A and A39R complexed with the Semaphorin-binding module of PlexinC1; both structures show two PlexinC1 molecules symmetrically bridged by Semaphorin dimers. PMID: 20727575
Sema7A is a potent stimulator of cytokine production, chemotaxis and superoxide release in monocytes PMID: 12193228
SEMA 7A might be a molecule involved in the terminal innervation of the dentin-pulp complex. PMID: 15907379
Polymorphisms in SEMA7A may play a role in decreased bone mineral density and increased risk of vertebral fracture. PMID: 16372136
beta1-integrins and Plexin C1 receptors are ligands for Semaphorin 7a, and that signaling by these receptors has opposing effects on Semaphorin 7a-induced dendrite formation. PMID: 17671519
Sema7A binds to human melanocytes through beta1-integrins and the Plexin C1 receptor PMID: 19318806

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Note=Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes.

Protein Families

Semaphorin family

Tissue Specificity

Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus

Database Links

HGNC: 10741

OMIM: 607961

KEGG: hsa:8482

STRING: 9606.ENSP00000261918

UniGene: Hs.24640

Code	CSB-YP021000HU
Abbreviation	Recombinant Human SEMA7A protein
MSDS	MSDS Datasheet
Size	$368
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Semaphorin-7A (SEMA7A)

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