Recombinant Human Serine/arginine-rich splicing factor 7 (SRSF7)

Code CSB-YP618089HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP618089HU
MSDS
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Source E.coli
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Code CSB-EP618089HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP618089HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP618089HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SRSF7
Uniprot No.
Alternative Names
9G8; AAG3; arginine/serine-rich 7; HSSG1; RBM37; Serine/arginine-rich splicing factor 7; Splicing factor 9G8; Splicing factor; Splicing factor, arginine/serine rich 7; SRSF7; SRSF7_HUMAN; ZCCHC20; ZCRB2
Species
Homo sapiens (Human)
Expression Region
1-238
Target Protein Sequence
MSRYGRYGGE TKVYVGNLGT GAGKGELERA FSYYGPLRTV WIARNPPGFA FVEFEDPRDA EDAVRGLDGK VICGSRVRVE LSTGMPRRSR FDRPPARRPF DPNDRCYECG EKGHYAYDCH RYSRRRRSRS RSRSHSRSRG RRYSRSRSRS RGRRSRSASP RRSRSISLRR SRSASLRRSR SGSIKGSRYF QSPSRSRSRS RSISRPRSSR SKSRSPSPKR SRSPSGSPRR SASPERMD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific.
Gene References into Functions
  1. role of SRSF7 in cell proliferation through regulating p21 levels. PMID: 27644562
  2. SRSF7 expression in cancer cells is regulated by microRNAs: short, non-coding RNAs that bind to 3'UTR of target genes and downregulate their expression. SRSF7 regulate proliferation of renal cancer cells.SRSF7 regulates expression of osteopontin. PMID: 27664584
  3. Genome-wide identification of CD95 antigen alternative splicing regulators, such as SRSF7, reveals links with iron homeostasis. PMID: 25482508
  4. Coexpression of Dyrk1A and splicing factor 9G8 leads to their translocation from the nucleus to the cytoplasm and suppresses their ability to regulate tau exon 10 splicing. PMID: 21215488
  5. Cyclic AMP-dependent protein kinase regulates 9G8-mediated alternative splicing of tau exon 10 PMID: 22677170
  6. Upregulation of total tau expression (SFRS7-independent) and tau exon 10 splicing (SFRS7-dependent), as shown in this study to be both affected by STOX1A, is known to have implications in neurodegeneration. PMID: 21755018
  7. These results indicate that 9G8 plays a key role in regulation of exon 10 splicing and imply a pathogenic role in neurodegenerative diseases. PMID: 17137791
  8. 9G8 was shown to enhance expression of unspliced RNA containing either the Mason-Pfizer monkey virus constitutive transport element PMID: 17513303
  9. eIF3f mediates restriction of HIV-1 expression through a set of factors that includes eIF3f, the SR protein 9G8, and the cyclin-dependent kinase 11 (CDK11). PMID: 19854136

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Splicing factor SR family
Tissue Specificity
Brain, liver, kidney and lung.
Database Links

HGNC: 10789

OMIM: 600572

KEGG: hsa:6432

STRING: 9606.ENSP00000325905

UniGene: Hs.309090

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