Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

PPP5C

Uniprot No.

P53041

Research Area

Epigenetics and Nuclear Signaling

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-499aa

Target Protein Sequence

AMAEGERTECAEPPRDEPPADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRSLAYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQECNKIVKQKAFERAIAGDEHKRSVVDSLDIESMTIEDEYSGPKLEDGKVTISFMKELMQWYKDQKKLHRKCAYQILVQVKEVLSKLSTLVETTLKETEKITVCGDTHGQFYDLLNIFELNGLPSETNPYIFNGDFVDRGSFSVEVILTLFGFKLLYPDHFHLLRGNHETDNMNQIYGFEGEVKAKYTAQMYELFSEVFEWLPLAQCINGKVLIMHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLLWSDPQPQNGRSISKRGVSCQFGPDVTKAFLEENNLDYIIRSHEVKAEGYEVAHGGRCVTVFSAPNYCDQMGNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMAYANTLLQLGMM
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

64.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This recombinant human serine/threonine-protein phosphatase 5 (PPP5C) is expressed in E. coli. The production process begins by introducing a gene fragment encoding the full-length mature human PPP5C protein (amino acids 2-499) into a suitable expression vector. The vector is designed to incorporate an N-terminal 10xHis-tag and a C-terminal Myc-tag for easy detection and purification of the recombinant protein. The recombinant construct is then transformed into E. coli cells, followed by the selection of transformed cells to ensure the presence of the recombinant plasmid. Subsequently, a large-scale culture is established, providing optimal conditions for protein expression. The expressed recombinant PPP5C protein is harvested from the cells and purified to achieve a high level of purity. SDS-PAGE analysis confirms the purity of the protein, which reaches up to 85%. When visualized on the gel, the recombinant Gpi protein migrates as a distinct band with an approximate molecular weight of 66 kDa.

Target Background

Function

Serine/threonine-protein phosphatase that dephosphorylates a myriad of proteins involved in different signaling pathways including the kinases CSNK1E, ASK1/MAP3K5, PRKDC and RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT. Implicated in wide ranging cellular processes, including apoptosis, differentiation, DNA damage response, cell survival, regulation of ion channels or circadian rhythms, in response to steroid and thyroid hormones, calcium, fatty acids, TGF-beta as well as oxidative and genotoxic stresses. Participates in the control of DNA damage response mechanisms such as checkpoint activation and DNA damage repair through, for instance, the regulation ATM/ATR-signaling and dephosphorylation of PRKDC and TP53BP1. Inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress. Plays a positive role in adipogenesis, mainly through the dephosphorylation and activation of PPARG transactivation function. Also dephosphorylates and inhibits the anti-adipogenic effect of NR3C1. Regulates the circadian rhythms, through the dephosphorylation and activation of CSNK1E. May modulate TGF-beta signaling pathway by the regulation of SMAD3 phosphorylation and protein expression levels. Dephosphorylates and may play a role in the regulation of TAU/MAPT. Through their dephosphorylation, may play a role in the regulation of ions channels such as KCNH2. Dephosphorylate FNIP1, disrupting interaction with HSP90AA1/Hsp90.

Gene References into Functions

This work offers insights of how cantharidin-like inhibitors interact with human PP5, potentially allowing the design of more specific and even less cytotoxic drugs for cancer treatments. PMID: 30280322
PP5 binds and dephosphorylates the elastic N2B-unique sequence (N2Bus) of titin in cardiomyocytes. PP5 is pathologically elevated and likely contributes to hypo-phosphorylation of N2Bus in failing human hearts. Furthermore, Hsp90-activated PP5 interacts with components of a sarcomeric, N2Bus-associated, mechanosensor complex, and blocks mitogen-activated protein-kinase signaling in this complex. PMID: 29343782
High PPP5C expression is associated with urinary bladder cancer. PMID: 28534961
PP5-dependent impairment of GRalpha function represents a novel mechanism driving GC insensitivity in ASM in severe asthma. PMID: 27501780
Our results showed 1) a critical role for PP5 in cytokine-induced resistance to GC-mediated eosinophil death, 2) supported the dependence of GCR phosphorylation on PP5 activity, and 3) revealed that PP5 is a target of the lipoxin A4-induced pathway countering cytokine-induced resistance to GCs in eosinophils. PMID: 27742828
the importance of PP5 in CRC cell growth PMID: 25322973
PPP5C could be essential for glioma cell growth. PMID: 25796168
Measles Virus Infection Inactivates Cellular Protein Phosphatase 5 with Consequent Suppression of Sp1 and c-Myc Activities. PMID: 26157124
We demonstrate for the first time that PPP5C is essential for growth of HCC cells, which suggests that inhibition of PPP5C by RNAi may be a potential therapeutic strategy for the treatment of hepatocellular carcinoma. PMID: 25326185
Data indicate that Cu-oxidized S100 calcium binding protein A4 (S100A4) interacted with S100 calcium binding protein A1 (S100A1) and prevented protein phosphatase 5 (PP5) activation. PMID: 25269953
These results demonstrate that Hsp70 recruits PP5 and activates its phosphatase activity which suggests dual roles for PP5 that might link chaperone systems with signaling pathways in cancer and development. PMID: 24327656
these results suggest that caveolin-1 is a novel physiological activator of PP5. PMID: 23352616
Chp-1 and melusin can interact with cochaperones PP5 and Sgt1 and with each other in an ATP-dependent manner PMID: 23184943
Suggest that Slim/KLHDC10 is an activator of ASK1, contributing to oxidative stress-induced cell death through the suppression of PP5. PMID: 23102700
proasthmatic cytokine-induced corticosteroid insensitivity in airway smooth muscle cells is due, in part, to PP5-mediated impairment of glucocorticoid receptor-Ser211 phosphorylation. PMID: 22592921
Findings are indicative of a regulatory role of PP5 in cardiac function. PMID: 20875921
S100A1 and permanently active S100P inhibited the apoptosis signal-regulating kinase 1 (ASK1) and PP5 interaction, resulting the inhibition of dephosphorylation of phospho-ASK1 by PP5 PMID: 22399290
genetic disruption of PP5 is associated with enhanced and prolonged phosphorylation of a single serine (Ser-345) on Chk1 PMID: 21921034
PP5 plays a crucial role in ATR-mediated repair of UV-induced DNA damage. PMID: 21144835
Specific binding of PP5 to activated Rac1 provides a direct mechanism by which PP5 can be stimulated and recruited to participate in Rac1-mediated signaling pathways. PMID: 19948726
PP5 suppresses a pathway regulating the expression of p21(waf1) PMID: 12519780
protein phosphatase 5 interacts with DNA-PKcs and dephosphorylates with surprising specificity at least two functional sites. PMID: 14734805
structure of Serine/threonine protein phosphatase-5(PP5c) provides a structural basis for explaining the exceptional catalytic proficiency of protein phosphatases. PMID: 15155720
PP5 is regulated by mTOR and activates apoptosis signal-regulating kinase 1 signaling PMID: 15218033
PP5 plays an important role in the survival of cells in a low oxygen environment by suppressing a hypoxia-induced ASK-1/MKK4/JNK signaling cascade that promotes an apoptotic response PMID: 15328343
Results describe the structure of the autoinhibited state of protein phosphatase 5, revealing mechanisms of tetratricopeptide repeat-mediated phosphatase inhibition and Hsp90- and arachidonic acid-induced stimulation of phosphatase activity. PMID: 15577939
PP5 plays a critical role in ATR-mediated checkpoint activation PMID: 16260606
We report the solution structure of a complex of the TPR domain of Ppp5 with the C-terminal pentapeptide of Hsp90.This may be important for the relief of autoinhibition in Ppp5 and for the mechanisms of TPR-mediated recognition of Hsp90 by other proteins. PMID: 16531226
PP5, casein kinase Iepsilon, and cryptochrome dynamically regulate the mammalian circadian clock PMID: 16790549
PP5 is a physiological regulator of Raf-1 signalling pathways. PMID: 16892053
Protein phosphatase 5 as a key dephosphorylation regulator of Raf-1 activation. PMID: 17084641
these studies indicate that elevated levels of PP5 protein occur in human breast cancer and suggest that PP5 over-expression may aid tumor progression. PMID: 18280813
domain amphiphilicity is of higher importance than amino acid sequence specificity in the determination of protein adsorption and interfacial activity. PMID: 18547097
PP5 plays an important role in the regulation of 53BP1 phosphorylation and activity in vivo PMID: 19176521
Estrogen inhibits glucocorticoid action via protein phosphatase 5 (PP5)-mediated glucocorticoid receptor dephosphorylation. PMID: 19586900

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Subcellular Location

Nucleus. Cytoplasm. Cell membrane.

Protein Families

PPP phosphatase family, PP-5 (PP-T) subfamily

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 9322

OMIM: 600658

KEGG: hsa:5536

STRING: 9606.ENSP00000012443

UniGene: Hs.654604

Code	CSB-EP018582HU
Abbreviation	Recombinant Human PPP5C protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Serine/threonine-protein phosphatase 5 (PPP5C)

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