Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

SERPINB4

Uniprot No.

P48594

Research Area

Cancer

Alternative Names

Leupin; Peptidase inhibitor 11; PI 11; PI-11; PI11; Protease inhibitor (leucine serpin); SCCA 2; SCCA-2; SCCA1; SCCA2; SCCA2/SCCA1 fusion protein; Serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 4; Serpin B4; Serpin peptidase inhibitor; clade B (ovalbumin); member 4; SERPINB 4; SERPINB4; SPB4_HUMAN; Squamous cell carcinoma antigen 1; Squamous cell carcinoma antigen 2

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-390aa

Target Protein Sequence

MNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTAQQISKVLHFDQVTENTTEKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYQFLQEYLDAIKKFYQTSVESTDFANAPEESRKKINSWVESQTNEKIKNLFPDGTIGNDTTLVLVNAIYFKGQWENKFKKENTKEEKFWPNKNTYKSVQMMRQYNSFNFALLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETCVDLHLPRFKMEESYDLKDTLRTMGMVNIFNGDADLSGMTWSHGLSVSKVLHKAFVEVTEEGVEAAAATAVVVVELSSPSTNEEFCCNHPFLFFIRQNKTNSILFYGRFSSP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

60.9kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human SERPINB4 contains amino acids 1-390. The theoretical molecular weight of the SERPINB4 protein is 60.9 kDa. The SERPINB4 protein was expressed in e.coli. The SERPINB4 coding gene included the N-terminal 6xHis-SUMO tag, which simplifies the detection and purification processes of the recombinant SERPINB4 protein in following stages of expression and purification.

Serpin B4 (SERPINB4), a member of the serine protease inhibitor (serpin) superfamily, plays a role in regulating proteolytic processes. SERPINB4 is primarily expressed in squamous epithelial tissues and has been implicated in various physiological and pathological conditions. SERPINB4 is known to inhibit specific serine proteases, thereby modulating processes like inflammation, tissue remodeling, and immune response. Research on SERPINB4 includes investigations into its involvement in cancer, particularly in squamous cell carcinomas, where its dysregulation may contribute to tumor progression and metastasis. Understanding the functions and regulatory mechanisms of SERPINB4 can provide insights into its potential as a therapeutic target in diseases associated with aberrant protease activity.

Target Background

Function

May act as a protease inhibitor to modulate the host immune response against tumor cells.

Gene References into Functions

The combination of SCCA-IgM with common clinical data shows promising diagnostic performance for the detection of Non-alcoholic steato-hepatitis in hepatitis C virus patients PMID: 29712495
Both periostin and SCCA may play a role in the pathogenesis of acute bronchitis due to respiratory syncytial virus PMID: 29122495
Serum SCCA2 levels reflected disease severity and clinical type of atopic dermatitis. Serum SCCA2 may thus be a relevant biomarker for atopic dermatitis. PMID: 28734739
Data show that oropharyngeal squamous cell carcinomas (OPSCCs) express granzyme inhibitors SERPINB1, SERPINB4 and SERPINB9 for cytotoxicity and the expression was not different between human papillomavirus (HPV)-positive and HPV-negative tumors. PMID: 26993499
Pediatric CNS-PNETs evade immune recognition by downregulating cell surface MHC-I and CD1d expression. Intriguingly, expression of SERPINB9, SERPINB1, and SERPINB4 is acquired during tumorigenesis in 29%, 29%, and 57% of the tumors PMID: 26963506
It is a promising serological marker for hepatocellular carcinoma. PMID: 24635038
our findings established that SCCA1 contributes to tumorigenesis by promoting EMT and a UPR-dependent induction of NF-kappaB and IL6 autocrine signaling that promotes a protumorigenic inflammation. PMID: 25213322
demonstrate the generation of Pso p27 from SCCA1 with extracts from psoriatic scale and even more remarkably, the generation of Pso p27 from SCCA1 in the presence of mast cell associated chymase PMID: 24560885
The results show that SCCA1 has diverse effects on many of the cellular events that characterize asthma and its role extends beyond protease inhibition. PMID: 23199842
The serum level of SCCA is effective for detecting inverted papilloma (IP), including recurrent IP. In contrast, the SCCA2/SCCA1 ratio is useful for detecting squamous cell carcinoma among other sinonasal diseases. PMID: 23168150
High serum SCCA1 levels are associated with lymph node metastasis, advanced tumor stage and tumor recurrence in oral cavity squamous cell carcinoma. PMID: 22576068
both HPV status and the SCCA2/SCCA1 mRNA ratio are independently associated with prognosis in HNSCC PMID: 22937809
Serpin B4 isoform overexpression is associated with aberrant epithelial proliferation and lung cancer in idiopathic pulmonary fibrosis. PMID: 22406480
Results indicate that cellular overexpression of SERPINB4 inhibits recombinant GrM-induced as well as NK cell-mediated cell death. PMID: 21857942
Data suggest that SCCA1 inhibits cell death induced by lysosomal injury while, on the other hand, it sensitizes cells to ER stress by activating caspase-8 independently of the death receptor apoptotic pathway. PMID: 21576355
Elevated serum SCCA1 is associated with squamous cell carcinoma in cervical cancer. PMID: 21338226
Transgenic mice expressing human SCCA1 showed higher liver regenerative potential compared to wild-type mice, supporting the dual role of this serpin as an anti-apoptotic and pro-proliferative stimulus for liver cells in vivo. PMID: 19956912
expression in various normal tissue types and in tumor cell lines, increase in expression induced by TNF-alpha, and role in protecting tumor cells against TNF-alpha induced apoptsis PMID: 12437110
SCCA2-like serpins mediate genetic predisposition to skin cancer in a mouse model and in humans. PMID: 12702576
SCCA2 acts as a cross-class serpin targeting an extrinsic cysteine proteinase from mites and may have a protective role against biological reactions caused by mites PMID: 14630915
SCCA variants are overexpressed in hepatocellular carcinoma independently of tumour etiology PMID: 14970861
SCCAs can alter invasive phenotype of cervical squamous cell carcinoma cells, probably by stimulating proMMP-9 production, and intact conformational structure of SCCAs is required for its stimulatory activity on proMMP-9 production PMID: 16211231
Up-regulation of squamous cell carcinoma antigen-1 (SCCA1) suppresses c-Jun NH2-terminal kinase-1 (JNK1) and thus blocks UV-induced keratinocyte apoptosis. PMID: 16549498
These results, in transgenic mice, suggest that SCAA1 might serve as an hepatitis B virus receptor or co-receptor and play an important role in mediating HBV entry into hepatocytes. PMID: 16820864
SCCA2 regulates cell migration and invasion via E-cadherin expression, suggesting that SCCA2 may be involved in cancer behavior such as invasion or metastasis. PMID: 17016656
SCCA2 may be involved in cancer behavior such as metastasis, and as such can be a useful marker in predicting lymph node metastasis. PMID: 18097581
fatty acid-binding protein-5, squamous cell carcinoma antigens 2, alpha-enolase, annexin II, apolipoprotein A-I and albumin were detected at a high level in Atopic dermatitis skin lesions, but scarcely in the normal controls PMID: 19339807

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Subcellular Location

Cytoplasm. Note=Seems to also be secreted in plasma by cancerous cells but at a low level.

Protein Families

Serpin family, Ov-serpin subfamily

Tissue Specificity

Squamous cells.

Database Links

HGNC: 10570

OMIM: 600518

KEGG: hsa:6318

STRING: 9606.ENSP00000343445

UniGene: Hs.123035

Code	CSB-EP021072HU
Abbreviation	Recombinant Human SERPINB4 protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Serpin B4 (SERPINB4)

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