Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
Signal recognition particle 54 kDa protein; Signal recognition particle 54kDa; SRP 54; SRP54; SRP54_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-504aa
Target Protein Sequence
MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-B2M-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Human SRP54 contains amino acids 1-504. The expected molecular weight for the SRP54 protein is calculated to be 69.7 kDa. The SRP54 protein was expressed in e.coli. The SRP54 coding gene included the N-terminal 6xHis-B2M tag, which simplifies the detection and purification processes of the recombinant SRP54 protein in following stages of expression and purification.
The human signal recognition particle 54 kDa protein (SRP54) is a key component of the signal recognition particle (SRP), which is involved in the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP54 is a GTPase that recognizes the signal sequence of nascent polypeptide chains emerging from the ribosome. It forms a complex with the SRP RNA and additional SRP proteins, facilitating the targeting of the ribosome-nascent chain complex to the ER membrane. Upon successful targeting, SRP54 mediates the transfer of the signal peptide to the translocon, allowing the protein to be translocated into the ER for further processing and maturation. The role of SRP54 is crucial for proper protein trafficking and secretion in eukaryotic cells, and its dysfunction can lead to defects in protein localization and contribute to various cellular disorders. Research areas involving SRP54 include protein translocation, cellular trafficking, and the molecular mechanisms underlying protein targeting to the ER.
