Recombinant Human Signal recognition particle subunit SRP54 (SRP54)

The region for expressing recombinant Human SRP54 contains amino acids 1-504. The expected molecular weight for the SRP54 protein is calculated to be 69.7 kDa. The SRP54 protein was expressed in e.coli. The SRP54 coding gene included the N-terminal 6xHis-B2M tag, which simplifies the detection and purification processes of the recombinant SRP54 protein in following stages of expression and purification.

The human signal recognition particle 54 kDa protein (SRP54) is a key component of the signal recognition particle (SRP), which is involved in the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP54 is a GTPase that recognizes the signal sequence of nascent polypeptide chains emerging from the ribosome. It forms a complex with the SRP RNA and additional SRP proteins, facilitating the targeting of the ribosome-nascent chain complex to the ER membrane. Upon successful targeting, SRP54 mediates the transfer of the signal peptide to the translocon, allowing the protein to be translocated into the ER for further processing and maturation. The role of SRP54 is crucial for proper protein trafficking and secretion in eukaryotic cells, and its dysfunction can lead to defects in protein localization and contribute to various cellular disorders. Research areas involving SRP54 include protein translocation, cellular trafficking, and the molecular mechanisms underlying protein targeting to the ER.