SRP54 Recombinant Monoclonal Antibody

SRP54 serves as a critical component of the signal recognition particle, the ribonucleoprotein complex responsible for targeting nascent secretory and membrane proteins to the endoplasmic reticulum. This 54 kDa subunit directly binds the signal peptide emerging from the ribosome, making it essential for understanding protein translocation pathways, ER stress responses, and the broader mechanisms governing cellular protein trafficking.

This recombinant monoclonal antibody, clone 25A3, offers the reproducibility that demanding signal transduction research requires. Generated against a synthetic peptide derived from human SRP54 and produced using recombinant technology, it provides sequence-defined specificity with consistent performance across experiments. The rabbit IgG format, purified by affinity chromatography, ensures reliable results whether you're establishing baseline protocols or conducting longitudinal studies where lot-to-lot variation could compromise data interpretation.

Validation studies demonstrate clear utility in both immunofluorescence and flow cytometry applications. Immunofluorescence staining of HepG2 hepatocellular carcinoma cells at 1:50 dilution reveals distinct subcellular localization patterns consistent with SRP54's role in the protein secretion machinery. Flow cytometry analysis using HeLa cells at 1:100 dilution shows well-resolved positive populations with clear separation from isotype control, confirming specific detection in fixed and permeabilized cell preparations. These validated human cell lines provide confidence for researchers working with similar experimental systems.

The unconjugated format allows flexibility in secondary antibody selection and detection system optimization. For investigators studying cotranslational protein targeting, ER-associated quality control mechanisms, or signal peptide recognition, this antibody provides a dependable tool for visualizing SRP54 distribution and expression in human cell models.