Product Details

Purity

>85% (SDS-PAGE)

Target Names

SLC13A5

Uniprot No.

Q86YT5

Alternative Names

Na(+)/citrate cotransporter; NaC2/NaCT; NaCT; Novel solute carrier family 13 (sodium dependent dicarboxylate transporter) (Slc13a2 or 3) member; S13A5_HUMAN; Slc13a5; Sodium coupled citrate transporter; Sodium-coupled citrate transporter; Sodium-dependent citrate transporter; Solute carrier family 13 (sodium dependent citrate transporter); member 5; Solute carrier family 13 member 5

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

High-affinity sodium/citrate cotransporter that mediates citrate entry into cells. Transports citrate in a Na(+)-dependent manner, transport process is electrogenic and recognizes the trivalent form of citrate rather than the divalent form. Although citrate is its main substrate, other intermediates of the citric acid cycle, such as succinate, fumarate, malate, oxaloacetate and alpha-ketoglutarate can serve as substrates but with a much lower affinity compared to citrate. Shows a substrate sensitivity in the order of citrate > malate ~ alpha-ketoglutarate > succinate ~ fumarate > oxaloacetate ~ isocitrate. Shows substantial citrate transporter activity when sodium ions are replaced by either potassium or choline ions. Transport activity is potentiated by lithium ions in the presence of low concentrations of citrate but is inhibited by lithium ions in the presence of high concentrations of citrate. Involved in the regulation of citrate levels in the brain.

Gene References into Functions

SLC13A5 is the second major gene associated with the clinical diagnosis of KTZS. PMID: 27600704
Data suggest that SLC13A5 plays a role in progression/cell proliferation of human hepatocellular carcinoma cells; here, RNA interference using shRNA against SLC13A5 decreased tumor burden in treatment of hepatocarcinoma in a xenograft tumor model in nude mice. PMID: 28655760
Data suggest that SLC13A5 plays a role in progression/proliferation of human hepatocellular carcinoma cells; RNA interference using shRNA against SLC13A5 decreased tumor burden in treatment of hepatocarcinoma in a xenograft tumor model in nude mice. [REVIEW] PMID: 28821606
Study identified additional SLC13A5 mutations in patients with chronic epilepsy starting in the neonatal period, with the mutations producing inactive Na+/citrate transporters. PMID: 27261973
Studies show that SLC13A5 is a transporter in the plasma membrane that mediates the uptake of citrate into cells. It is expressed in hepatocytes, neurons, and spermatozoa. Its loss-of-function mutations are associated with neonatal epilepsy in humans. This is a single-gene disease with epilepsy resulting solely from the inactivity of SLC13A5. [review] PMID: 28264506
In infants presenting with therapy resistant seizures in the first days after birth, without a clear history of hypoxic-ischemic encephalopathy, but with Punctate White Matter Lesions on their neonatal MRI, a diagnosis of SCL13A5 related epileptic encephalopathy should be considered. PMID: 27913086
Discovery and characterization of novel inhibitors of the sodium-coupled citrate transporter (NaCT or SLC13A5). PMID: 26620127
Eight patients from four families with SLC13A5 mutation are described. They have neonatal epilepsy, tooth hypoplasia, and developmental delay. PMID: 26384929
SLC13A5 is a novel target gene of PXR and may contribute to drug-induced steatosis and metabolic disorders in humans. PMID: 25628225
Screening of 68 additional unrelated individuals with early-onset epileptic encephalopathy for SLC13A5 mutations led to identification of one additional subject with heterozygous mutations of SLC13A5 and a similar clinical presentation as index subjects PMID: 24995870
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
This paper describes the cloning and functional characterization of the human Na(+)-coupled citrate transporter (NaCT). PMID: 12445824
mediates the utilization of extracellular citrate for fat synthesis in human liver cells, and that the process is stimulated by lithium PMID: 12826022
Expression and function of NaCT in a cell line and in primary hepatocytes. PMID: 16973915

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Involvement in disease

Epileptic encephalopathy, early infantile, 25 (EIEE25)

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

SLC13A/DASS transporter (TC 2.A.47) family, NADC subfamily

Tissue Specificity

Expressed most predominantly in the liver, with moderate expression detectable in the brain and testis.

Database Links

HGNC: 23089

OMIM: 608305

KEGG: hsa:284111

STRING: 9606.ENSP00000406220

UniGene: Hs.399496

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Recombinant Human Solute carrier family 13 member 5 (SLC13A5), partial

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