Recombinant Human Spermine oxidase (SMOX)

Code CSB-YP021844HU
MSDS
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Source Yeast
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Code CSB-EP021844HU
MSDS
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Source E.coli
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Code CSB-EP021844HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021844HU
MSDS
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Source Baculovirus
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Code CSB-MP021844HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SMOX
Uniprot No.
Alternative Names
SMOX; C20orf16; SMO; UNQ3039/PRO9854; Spermine oxidase; EC 1.5.3.16; Polyamine oxidase 1; PAO-1; PAOh1
Species
Homo sapiens (Human)
Expression Region
1-555
Target Protein Sequence
MQSCESSGDS ADDPLSRGLR RRGQPRVVVI GAGLAGLAAA KALLEQGFTD VTVLEASSHI GGRVQSVKLG HATFELGATW IHGSHGNPIY HLAEANGLLE ETTDGERSVG RISLYSKNGV ACYLTNHGRR IPKDVVEEFS DLYNEVYNLT QEFFRHDKPV NAESQNSVGV FTREEVRNRI RNDPDDPEAT KRLKLAMIQQ YLKVESCESS SHSMDEVSLS AFGEWTEIPG AHHIIPSGFM RVVELLAEGI PAHVIQLGKP VRCIHWDQAS ARPRGPEIEP RGEGDHNHDT GEGGQGGEEP RGGRWDEDEQ WSVVVECEDC ELIPADHVIV TVSLGVLKRQ YTSFFRPGLP TEKVAAIHRL GIGTTDKIFL EFEEPFWGPE CNSLQFVWED EAESHTLTYP PELWYRKICG FDVLYPPERY GHVLSGWICG EEALVMEKCD DEAVAEICTE MLRQFTGNPN IPKPRRILRS AWGSNPYFRG SYSYTQVGSS GADVEKLAKP LPYTESSKTA PMQVLFSGEA THRKYYSTTH GALLSGQREA ARLIEMYRDL FQQGT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.
Gene References into Functions
  1. Data suggest that intermolecular disulfide bond links spermine oxidase (SMOX) molecules to form the homodimer and plays a critical role in the stabilization of the overall three-dimensional SMOX structure. PMID: 29138259
  2. These results indicate a protective role for miR-124 through the inhibition of SMOX-mediated DNA damage in the etiology of H. pylori-associated gastric cancer. PMID: 27041578
  3. effect of Tat on Nrf2 activation in human neuroblastoma cells and the role of NMDA receptor and spermine oxidase on Tat-induced nuclear factor erythroid 2-related factor 2 (Nrf2) activation PMID: 26895301
  4. During H. pylori infection, the enzyme spermine oxidase (SMOX) is induced, which generates hydrogen peroxide from the catabolism of the polyamine spermine, and increases gastric cancer risk. PMID: 25174398
  5. study postulates a mechanism for SAT1 and SMOX down-regulation by post-transcriptional activity of miRNAs. PMID: 24025154
  6. Tat was found to induce reactive oxygen species production and to affect cell viability in SH-SY5Y cells, these effects being mediated by spermine oxidase (SMO) PMID: 23665428
  7. Spermine oxidase mediates the gastric cancer risk associated with Helicobacter pylori CagA. PMID: 21839041
  8. each gene was associated with at least one main outcome: anxiety (SAT1, SMS), mood disorders (SAT1, SMOX), and suicide attempts (SAT1, OATL1). PMID: 21152090
  9. Spermine oxidase (SMO) has a role in response to BENSpm and CPENSpm in breast tumor cells PMID: 20946629
  10. Increased expression of spermine oxidase is associated with ulcerative colitis. PMID: 20127992
  11. the genetic and epigenetic factors examined in this study show little influence on the expression level of SMOX in suicide completers. PMID: 20059804
  12. Knockdown studies suggest that induction of SSAT and SMO is correlated with the antiproliferative effects of BENSpm with 5-FU or paclitaxel in MDA-MB-231 cells. PMID: 19727732
  13. Fully protonated forms of the inhibitors and the unprotonated form of an amino acid residue with a pK(a) of approximately 7.4 in the active site are preferred for binding. PMID: 20000632
  14. There are at least four isoenzymes of human PAO, each with different biochemical characteristics and roles in polyamine catabolism PMID: 12398765
  15. The results of these studies are consistent with the hypothesis that polyamine oxidase 1 (PAOh1/SMO) represents a new addition to the polyamine metabolic pathway. PMID: 12727196
  16. SSAT and SMO(PAOh1) activities are the major mediators of the cellular response of breast tumor cells to polyamines while PAO plays little or no role in this response PMID: 16207710
  17. Tissues from patients with prostate cancer and prostatic intraepithelial neoplasia exhibit increased spermine oxidase expression. PMID: 18302221
  18. analysis of nuclear localization of human spermine oxidase isoforms PMID: 18422650

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Subcellular Location
[Isoform 1]: Cytoplasm. Nucleus.; [Isoform 4]: Cytoplasm. Nucleus.; [Isoform 6]: Cytoplasm. Nucleus.
Protein Families
Flavin monoamine oxidase family
Tissue Specificity
Widely expressed. Expressed in human tumor cell lines. Isoform 4 is only found in an embryonal kidney cell line.
Database Links

HGNC: 15862

OMIM: 615854

KEGG: hsa:54498

STRING: 9606.ENSP00000307252

UniGene: Hs.433337

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