CD47|Proteinase K|EGFR|BCMA|PD-L1|CD276|ACE2|TMPRSS2|Exosome Isolation Kits
Code | CSB-YP006400HU |
Size |
US$1916Purchase it in Cusabio online store (only available for customers from the US) |
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Description |
A DNA sequence encoding the full-length human Steroid 21-hydroxylase (CYP21A2) protein was fused with a 6xHis tag at the N-terminus and then expressed in the yeast. The forming protein is the Recombinant Human CYP21A2 protein, which contains 494 amino acids. Its purity was measure by SDS-PAGE and reached up to 90%. This in-stock recombinant CYP21A2 protein is not only used for antibody synthesis but also in the studies of signal transduction. CYP21A2, a member of the microsomal cytochrome P450 family, is a critical enzyme of the glucocorticoid and mineralocorticoid biosynthesis in the adrenal cortex. Mutations in the CYP21A2 gene lead to abrupt truncations of the protein per se and further severely damage enzymatic activity, resulting in salt-wasting (SW) congenital adrenal hyperplasia (CAH). |
Purity | Greater than 90% as determined by SDS-PAGE. |
Target Names | CYP21A2 |
Uniprot No. | P08686 |
Research Area | Signal Transduction |
Alternative Names | CYP21; 21 OHase; 21-OHase; CA21H ; CAH1; CP21A_HUMAN; CPS1; CYP21A 2; CYP21A2; CYP21B; Cytochrome P-450c21; Cytochrome P450 21; Cytochrome P450 C21B; Cytochrome P450 XXI; Cytochrome P450; family 21; subfamily A; polypeptide 2; Cytochrome P450; subfamily XXIA (steroid 21 hydroxylase; congenital adrenal hyperplasia); polypeptide 2; Cytochrome P450-C21; Cytochrome P450-C21B; P450 C21; P450 C21B; P450c21B; Steroid 21 hydroxylase; Steroid 21 monooxygenase; Steroid 21-hydroxylase |
Species | Homo sapiens (Human) |
Source | Yeast |
Expression Region | 1-494aa |
Target Protein Sequence | MLLLGLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDVVVLNSKRTIEEAMVKKWADFAGRPEPLTYKLVSKNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQNQ Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
Mol. Weight | 57.9kDa |
Protein Length | Full Length |
Tag Info |
N-terminal 6xHis-tagged |
Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Troubleshooting and FAQs |
Protein FAQs |
Storage Condition | Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Lead Time | Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Datasheet & COA | Please contact us to get it. |
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Function | Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids |
Gene References into Functions |
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Involvement in disease | Adrenal hyperplasia 3 (AH3) |
Subcellular Location | Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane, Peripheral membrane protein |
Protein Families | Cytochrome P450 family |
Database Links |
HGNC: 2600 OMIM: 201910 KEGG: hsa:1589 STRING: 9606.ENSP00000408860 UniGene: Hs.654479 |