Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

TACR1

Uniprot No.

P25103

Research Area

Neuroscience

Alternative Names

Neurokinin 1; Neurokinin 1 Receptor; NK 1 receptor; NK 1R; NK-1 receptor; NK-1R; NK1 receptor; NK1R; NK1R_HUMAN; NKIR; SPR; Substance P receptor; Substance-P receptor; TAC 1R; TAC1R; Tachykinin 1 receptor (substance P receptor neurokinin 1 receptor); Tachykinin receptor 1 (substance P receptor neurokinin 1 receptor); Tachykinin receptor 1; TACR1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-29aa

Target Protein Sequence

MDNVLPVDSDLSPNISTNTSEPNQFVQPA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

30.1kDa

Protein Length

partial

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-29 form the expressed segment for recombinant Human TACR1. The theoretical molecular weight of the TACR1 protein is 30.1 kDa. Expression of this TACR1 protein is conducted in e.coli. The TACR1 coding gene included the N-terminal GST tag, which simplifies the detection and purification processes of the recombinant TACR1 protein in following stages of expression and purification.

The substance P receptor is officially known as the Tachykinin receptor 1 (TACR1) or neurokinin-1 receptor (NK1R). This receptor is a G protein-coupled receptor that binds specifically to substance P, a neuropeptide involved in neurotransmission, inflammation, and pain signaling. TACR1 is primarily expressed in the central and peripheral nervous systems and plays a crucial role in mediating the effects of substance P. Upon binding of substance P to TACR1, various intracellular signaling pathways are activated, leading to neurogenic inflammation, vasodilation, and modulation of pain perception. The TACR1 receptor is implicated in numerous physiological and pathological processes, making it a significant target for research in areas such as neuroscience, pain biology, and immunology. Understanding TACR1 function is crucial for developing therapeutic interventions related to pain management and inflammatory disorders.

Target Background

Function

This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.

Gene References into Functions

The neuropeptide, substance P (SP), is the physiological ligand of the neurokinin-1 (NK-1) receptor that is consistently overexpressed in glioblastoma cells. The aim of this work was to study physico-chemical and biological properties of different SP analogues labeled with technetium-99m and lutetium-177 radionuclides. PMID: 30301182
NK-1RA is recommended to use in carboplatin-based chemotherapy, not oxaliplatin-based chemotherapy PMID: 30019968
Study revealed extensive distribution of SP and NK1R in nuclei intimately related to cardiorespiratory function and autonomic control, with significant co-distribution and co-localization with 5-HT in the medullary network in the normal human infant during development. A trend for NK1R binding to decrease with age was observed with significantly higher binding in premature and male infants. PMID: 29331576
These results reveal a critical role for endosomal signaling of the NK1R in the complex pathophysiology of pain. PMID: 28566424
The percentages of SP+ and NK1R+ expression populations of monocytes, helper T cells, natural killer T cells and basophils in peripheral blood of eczema patients were markedly elevated. PMID: 28154998
Expression of NK1R increases in the eosinophils of chronic spontaneous urticaria (CSU) patients. Blockers of NK1R might be used for CSU treatment. PMID: 28871952
NK-1R may positively regulate melanogenesis through Wnt/beta-catenin signaling pathway. PMID: 27835606
This study revealed for the first time an increase of Mast Cells -nerve association and NK1R expression on Mast Cells during Allergic Rhinitis as well as nerve fibres containing receptors for mass cells. PMID: 28030866
Kinase activation led to increased MMP-2 and MT1-MMP expression and melanoma cell migration induced by hHK-1. Thus, hHK-1 and the NK1 receptor are critical to melanoma cell migration and each may be a promising chemotherapeutic target PMID: 27458061
Within a small cohort of patients who presented for treatment,a novel gene was identified that appears to contribute to anorexia nervosa pathophysiology, TACR1. PMID: 28291261
NK1 receptor expression is increased in colonic tissues from experimental colitis models and in the colon of Irritable Bowel Disease patients. PMID: 28774868
High Truncated Neurokinin-1 Receptor expression is associated with Neuroblastoma. PMID: 29061788
Abnormalities in substance P neurokinin-1 receptor binding in key brainstem nuclei is associated with sudden infant death syndrome. PMID: 28931039
TGF-beta1 down-regulates the gene expression of the full-length variant of NK-1 R in human keratocytes, which might impact its signaling pathway and thus explain the known delay in internalization after activation by SP seen with TGF-beta1 treatment. PMID: 26673553
results suggest that ARRB1 plays an essential role in NK1R-mediated cell proliferation and G2/M transition in glioblastoma cells. Interference with ARRB1-mediated signaling via NK1R may have potential significance for therapeutic strategies targeting glioblastoma. PMID: 28341744
up-regulated in nasal mucosa at contact point in intranasal mucosal contact point headache PMID: 26817501
We showed that pretreating a cardiomyocyte cell line (H9C2) and a TNBC cell line (MDA-MB 231) with aprepitant, a SP receptor antagonist that is routinely used to treat chemotherapy-associated nausea, decreased DOX-induced reduction of viability, apoptotic cell death, and ROS production in cardiomyocytes PMID: 26981525
These findings reveal important insight into the molecular mechanisms of the NK1R complex as a critical component in a model of pediatric liver cancer and may support the development of novel therapeutic interventions for hepatoblastoma PMID: 26516161
Report absence of full-length neurokinin-1 receptor protein expression by cutaneous T cells in mycosis fungoides. PMID: 25783846
Inhibition of CaV2.3 channels by NK1 receptors is sensitive to membrane cholesterol but insensitive to caveolin-1. PMID: 25204428
NK-1 activation could enhance EGFR expression and phosphorylation, and then activate EGFR related Erk/MAPK and Akt pathway. PMID: 25817575
The single nucleotide polymorphisms in the TACR1 gene are associated with sex differences in postoperative nausea and vomiting. PMID: 26012530
Results show the existence of a relevant alteration in NK-1R expression in bipolar disorder patients and further suggest Substance P involvement in BD. PMID: 25841180
Immunohistochemical NK-1R and pEGFR positivity with VDR negativity can be used to identify areas of sporadic colorectal neoplasia. PMID: 25684939
Hepatoblastoma cells predominantly expressed the truncated splice variant of NK1R. PMID: 24412605
Findings indicate a role of the TACR1 gene in the development of sicca syndrome in subjects affected with fibromyalgia. PMID: 25786041
NK-1R activation by substance P stimulation promotes growth and migration of esophageal squamous cell carcinoma cells. PMID: 25659767
Different NK1R subtype profiles of glomerular basement membrane (GBM) cell lines appear to influence the binding of SP conjugates and their cell internalization properties. PMID: 24552486
Down-regulation of NK1R-Tr may be a key factor in maintaining the quiescent phenotype of breast cancer cells among bone marrow stroma. PMID: 25130457
The association with TACR1 and bipolar affective disorder, alcohol dependence syndrome, and attention-deficit hyperactivity disorder suggests a shared molecular pathophysiology between these affective disorders. PMID: 24817687
NK-1 receptors are expressed in uveal melanomas and uveal melanoma cell lines. PMID: 24548567
TACR1 expression level was differentially altered in Crohn disease and ulcerative colitis patients. PMID: 23827863
Residues 11-21 and residues 170-193 of NK1 receptor are binding sites for substance P. PMID: 24831006
the primary function of betaARRs and ECE-1 in SP-dependent inflammatory signaling is to promote resensitization, which allows the sustained NK1R signaling from the plasma membrane that drives inflammation PMID: 24898255
we demonstrated for the first time that the NK1R is expressed in human osteosarcoma cell line MG63 and that this receptor can be targeted with NK1R antagonists both in vitro as well as in vivo. PMID: 24190675
data confirm the overall inflammatory nature of HIV-associated neuropathology, and a decrease in the expression of SP and NK1R-F. PMID: 23916293
NK1 receptors may be activated, or up-regulated in the peripheral, painful tissue of a chronic pain condition. PMID: 24155873
It seems that a common mechanism for cancer cell proliferation mediated by SP and the NK-1 receptor is triggered, as well as a common mechanism exerted by NK-1 receptor antagonists on tumor cells, i.e. apoptosis. PMID: 23933301
Roles of full-length and truncated neurokinin-1 receptor on tumor progression and distant metastasis in human breast cancer. PMID: 23807418
This suggests that potential pharmacological interventions that target NK-1R signaling may be most effective for treating distinct symptoms in IBD and IBS. PMID: 23582152
High NK-1 Receptor expression is associated with breast cancer. PMID: 24030979
NK1R forms physical complexes with NET. PMID: 23979140
Each of the 5 single nucleotide polymorphism(SNP)s in the TACR1 gene is significantly related to alcohol dependence severity. PMID: 23078527
Our results suggest that SP plays an important role in the development of pancreatic cancer metastasis and PNI, and blocking the SP/NK-1R signaling system is a novel strategy for the treatment of pancreatic cancer. PMID: 23345604
NK1R expression is induced in ectopic endometrial tissue by peritoneal TNFalpha. PMID: 23553861
NK1R expression correlates positively with plasma insulin, fatty acid, and complement C3 and negatively with adiponectin, CEBPalpha, CEBPbeta, and peroxisome proliferator-activated receptor-gamma mRNA expression in omental, but not subcutaneous, adipose tissue. PMID: 23257919
PAR(2) has a higher apparent affinity for both beta-arrestins than does NK1R, recruits them at a faster rate, and exhibits more rapid desensitization of the G-protein signal. PMID: 23235155
NK1R is a potential regulator of human glioma cell migration by the up-regulation of MMP-2 and MT1-MMP PMID: 23166329
Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells PMID: 23024816
investigation of age and sex influences on NK1 receptor expression in brain tissues PMID: 22225860

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Database Links

HGNC: 11526

OMIM: 162323

KEGG: hsa:6869

STRING: 9606.ENSP00000303522

UniGene: Hs.633301

Code	CSB-EP023068HU
Abbreviation	Recombinant Human TACR1 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Substance-P receptor (TACR1), partial

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