The recombinant Human TIGIT protein is encoded by the gene of TIGIT (22-141aa). The gene of TIGIT was cloned in a system (E.coli) that supported the expression of TIGIT and translation of messenger RNA. Modification of TIGIT by recombinant DNA technology could lead to the expression of the target protein. The protein was fused with N-terminal 6xHis-SUMO tag in the production. The purity is 90% determined by SDS-PAGE. And the activity has been verified.
TIGIT (VSIG9, VSTM3) is a protein coding gene that encodes T-cell immunoreceptor with Ig and ITIM domains. According to some studies, TIGIT may have the following features.
TIGIT, an inhibitory receptor expressed on lymphocytes, has recently been proposed as a major emerging target for cancer immunotherapy. TIGIT has an intrinsic suppressive function of T cells. The surface protein TIGIT inhibits T cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT is a key inhibitor of the cancer immune cycle. The immunosensor TIGIT regulates antitumor and antiviral CD8+ T cell effector functions. The TIGIT/CD226 axis regulates human T cell function. Interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. TIGIT, a new immunotherapy target, is moving from the lab to the bedside.
||Greater than 90% as determined by SDS-PAGE.
||DKFZp667A205; ENSMUSG00000071552; FLJ39873; RGD1563191; T cell immunoreceptor with Ig and ITIM domains; TIGIT; TIGIT_HUMAN; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3; Washington University cell adhesion molecule; WUCAM
||Homo sapiens (Human)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.