Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
FACS assay shows that Human TIGIT can bind to 293F cell overexpressing human CD155.
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
22-141aa
Target Protein Sequence
MMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIP
Tag Info
C-terminal hFc-Myc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
CUSABIO expressed the human TIGIT residues 22-141 carrying a C-terminal hFc-Myc tag in mammalian cells. The product is the recombinant human TIGIT protein. It is an active protein with high purity (>95%, SDS-PAGE) and low endotoxin (<1.0 EU/ug protein, LAL method). It has an apparent molecular weight of 46 kDa due to glycosylation. In the FACS assay, this TIGIT protein can bind to 293F cell overexpressing human CD155. And it is in stock so that there is no preparation waiting period.
TIGIT, a co-inhibitory molecule on T cells, exerts immunosuppressive effects by competing with CD226 for the same CD155 ligand. Accumulating roles demonstrate that TIGIT plays a key role in limiting adaptive and innate immunity against tumors.