Recombinant Human TATA box-binding protein-associated factor RNA polymerase I subunit A (TAF1A)

Code CSB-YP614895HU
MSDS
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Source Yeast
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Code CSB-EP614895HU
MSDS
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Source E.coli
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Code CSB-EP614895HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP614895HU
MSDS
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Source Baculovirus
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Code CSB-MP614895HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TAF1A
Uniprot No.
Alternative Names
MGC:17061; RAFI48; RNA polymerase I-specific TBP-associated factor 48 kDa; SL1 48kD subunit; SL1; Taf1a; TAF1A_HUMAN; TAFI48; TATA box binding protein (TBP) associated factor RNA polymerase I, A 48kDa; TATA box binding protein (Tbp)-associated factor, RNA polymerase I, A; TATA box binding protein associated factor 1A; TATA box binding protein associated factor RNA polymerase I subunit A; TATA box-binding protein-associated factor 1A; TATA box-binding protein-associated factor RNA polymerase I subunit A; TBP associated factor RNA polymerase I 48 kDa; TBP-associated factor 1A; Transcription factor SL1; Transcription initiation factor SL1/TIF-IB subunit A
Species
Homo sapiens (Human)
Expression Region
1-450
Target Protein Sequence
MSDFSEELKG PVTDDEEVET SVLSGAGMHF PWLQTYVETV AIGGKRRKDF AQTTSACLSF IQEALLKHQW QQAAEYMYSY FQTLEDSDSY KRQAAPEIIW KLGSEILFYH PKSNMESFNT FANRMKNIGV MNYLKISLQH ALYLLHHGML KDAKRNLSEA ETWRHGENTS SREILINLIQ AYKGLLQYYT WSEKKMELSK LDKDDYAYNA VAQDVFNHSW KTSANISALI KIPGVWDPFV KSYVEMLEFY GDRDGAQEVL TNYAYDEKFP SNPNAHIYLY NFLKRQKAPR SKLISVLKIL YQIVPSHKLM LEFHTLLRKS EKEEHRKLGL EVLFGVLDFA GCTKNITAWK YLAKYLKNIL MGNHLAWVQE EWNSRKNWWP GFHFSYFWAK SDWKEDTALA CEKAFVAGLL LGKGCRYFRY ILKQDHQILG KKIKRMKRSV KKYSIVNPRL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.
Gene References into Functions
  1. This study identifies the first nuclear import sequence within the TBP-Associated Factor subunits of Selectivity Factor 1. PMID: 15113842
  2. The authors demonstrate the interaction of both RNA polymerase I and III with hepatitis delta virus RNA, both in vitro and in human cells. PMID: 19246067
Subcellular Location
Nucleus.
Database Links

HGNC: 11532

OMIM: 604903

KEGG: hsa:9015

STRING: 9606.ENSP00000327072

UniGene: Hs.153088

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