Recombinant Human Transcription elongation factor, mitochondrial (TEFM)

Code CSB-YP857011HU
MSDS
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Source Yeast
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Code CSB-EP857011HU
MSDS
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Source E.coli
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Code CSB-EP857011HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP857011HU
MSDS
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Source Baculovirus
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Code CSB-MP857011HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TEFM
Uniprot No.
Alternative Names
TEFM; C17orf42; Transcription elongation factor; mitochondrial
Species
Homo sapiens (Human)
Expression Region
36-360
Target Protein Sequence
KSTTP KKITPNVTFC DENAKEPENA LDKLFSSEQQ ASILHVLNTA STKELEAFRL LRGRRSINIV EHRENFGPFQ NLESLMNVPL FKYKSTVQVC NSILCPKTGR EKRKSPENRF LRKLLKPDIE RERLKAVNSI ISIVFGTRRI AWAHLDRKLT VLDWQQSDRW SLMRGIYSSS VYLEEISSII SKMPKADFYV LEKTGLSIQN SSLFPILLHF HIMEAMLYAL LNKTFAQDGQ HQVLSMNRNA VGKHFELMIG DSRTSGKELV KQFLFDSILK ADPRVFFPSD KIVHYRQMFL STELQRVEEL YDSLLQAIAF YELAVFDSQP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription elongation factor which increases mitochondrial RNA polymerase processivity. Regulates transcription of the mitochondrial genome, including genes important for the oxidative phosphorylation machinery.
Gene References into Functions
  1. Data provide insights into target specificity of TEFM and mechanisms by which it regulates the switch between transcription and replication of mitochondrial DNA. PMID: 29033127
  2. Data indicate transcription elongation factor (TEFM) as an essential component of the mitochondrial transcription machinery. PMID: 25690892
  3. study found interaction of TEFM with mitochondrial RNA polymerase and nascent transcript prevents generation of replication primers and increases transcription processivity and serves as a molecular switch between replication and transcription, which appear to be mutually exclusive processes in mitochondria PMID: 25635099
  4. The TEFM protein is proposed to be a critical component of the transcription apparatus of human mitochondria. PMID: 21278163
Subcellular Location
Mitochondrion matrix. Mitochondrion matrix, mitochondrion nucleoid.
Protein Families
TEFM family
Database Links

HGNC: 26223

OMIM: 616422

KEGG: hsa:79736

UniGene: Hs.317340

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