Product Details

Purity

>85% (SDS-PAGE)

Target Names

TNPO3

Uniprot No.

Q9Y5L0

Alternative Names

Transportin-3; Importin-12; Imp12; Transportin-SR; TRN-SR; TNPO3 IPO12

Species

Homo sapiens (Human)

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Related Products

Target Background

Function

Importin, which transports target proteins into the nucleus. Specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1 and SFRS2, by recognizing phosphorylated SR domains. Also mediates the nuclear import of serine/arginine (SR) protein CPSF6, independently of CPSF6 phosphorylation. The nuclear import process is regulated by the small GTPase Ran that partitions between cytoplasm and nucleus in the predominantly GDP- and GTP-bound form, respectively. Importin associates with target cargo proteins in the cytoplasm, and the competitive binding of GTP-bound Ran induces the release of cargos in the nucleus.; (Microbial infection) Involved in immunodeficiency virus (HIV-1) infection by importing the pre-integration complex (PIC) into the nucleus. Required for a nuclear maturation step of HIV-1 prior to integration.

Gene References into Functions

Combining these results with small-angle X-ray scattering data for the complex of TRN-SR2 with truncated integrase, we propose a molecular model of the complex. We speculate that nuclear import of the PIC may proceed concurrently with the normal nuclear transport. PMID: 28356354
IRF5-TNPO3 genetic variation is associated systemic lupus erythematosus. PMID: 25205108
Data suggest that the HIV-1 integrase (IN)/transportin-SR2 (TRN-SR2) interaction interface is a potential target for antiviral therapy. PMID: 25063804
Tnpo3 mutants that are not able to interact with cleavage and polyadenylation specificity factor 6 do not facilitate HIV-1 infectivity, suggesting a potential route of pharmacological intervention in the treatment of AIDS. PMID: 24449914
TNPO3 mutation is the cause of limb-girdle muscular dystrophy 1F, expands knowledge of the molecular basis of muscular dystrophies and bolsters the importance of defects of nuclear envelope proteins as causes of inherited myopathies. PMID: 23543484
The TNPO3 gene is mapped within the Limb-girdle muscular dystrophy 1F critical interval and its 923-amino acid human gene product is also expressed in skeletal muscle. PMID: 23667635
a model wherein one monomer of TRN-SR2 is bound to one monomer of RanGTP. PMID: 23878195
In skeletal muscle of limb-girdle muscular dystrophy 1F individuals, expression of transportin 3 indicates altered transportin 3 function. PMID: 23543484
These results suggest that TNPO3 and cyclophilin A facilitate HIV-1 infection by coordinating proper uncoating of the core in target cells. PMID: 23097435
Identification of residues in the C-terminal domain of HIV-1 integrase that mediate binding to the transportin-SR2 protein. PMID: 22872638
TNPO3 can directly engage the HIV-1 IN tetramer prebound to the cognate DNA. PMID: 22872640
Transportin 3 and importin alpha act as receptors and are required for effective nuclear import of HIV-1 integrase in virus-infected cells. PMID: 21326825
TNPO3 interacts with HIV-1 gag in the cytoplasm to assist HIV-1 infection after nuclear import. PMID: 22398280
TNPO3 promotes HIV-1 infectivity at a step in the virus life cycle that is detectable after the preintegration complex arrives in the nucleus and capsid is the viral determinant for TNPO3 dependence. PMID: 22145813
TNPO3 binds to a surface of monomeric HIV-1 integrase that remains exposed after tetramerization. PMID: 22176773
a novel nuclear localization signal and mechanism for serpinF1 nuclear import PMID: 22028839
The authors demonstrated that TNPO3 was required by several lentiviruses for nuclear import. PMID: 21976643
Tnp3 binds to tRNAs and capsids proteins, and is required for efficient HIV-1 integration. PMID: 21901095
Here the authors discover a link between the viral entry of HIV and its interaction with TRN-SR2. PMID: 21276267
capsid, not integrase, is the dominant viral factor that dictates transportin 3 dependency during HIV-1 infection PMID: 19846519
Results imply that the mechanism by which SR proteins are imported to the nucleus is conserved between Drosophila and humans and involves the nuclear import receptor transportin-SR PMID: 12134081

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Involvement in disease

Limb-girdle muscular dystrophy 1F (LGMD1F)

Subcellular Location

Nucleus envelope. Cytoplasm.

Tissue Specificity

Expressed in skeletal muscle.

Database Links

HGNC: 17103

OMIM: 608423

KEGG: hsa:23534

STRING: 9606.ENSP00000265388

UniGene: Hs.193613

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Recombinant Human Transportin-3 (TNPO3 IPO12)

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