TNPO3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA191482A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: hela whole cell lysate(30µg), A549 whole cell lysate(30µg), K562 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), U251 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes:Transportin 3antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 100 kDa Observed band size: 104 kDa Exposure time：1min IHC image of CSB-RA191482A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA191482A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A431 cells stained with CSB-RA191482A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9Y5L0

Target Names

TNPO3

Alternative Names

ibody; Imp12 antibody; Importin 12 antibody; Importin-12 antibody; IPO12 antibody; MTR10A antibody; TNPO3 antibody; TNPO3_HUMAN antibody; Transportin 3 antibody; Transportin SR antibody; Transportin-3 antibody; Transportin-SR antibody; TRN SR antibody; TRN-SR antibody; TRN-SR2 antibody; TRNSR antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human TNPO3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Transportin-3 (TNPO3), also known as Importin-12, serves as a critical nuclear import receptor that facilitates the transport of serine/arginine-rich splicing factors into the nucleus. This karyopherin family member plays essential roles in pre-mRNA splicing regulation and has garnered significant research attention due to its involvement in HIV-1 infection, where it mediates nuclear import of the viral pre-integration complex. Understanding TNPO3 function provides valuable insights into both fundamental nuclear transport mechanisms and viral pathogenesis.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human TNPO3, offers the reproducibility and consistency that demanding research applications require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental results remain comparable across extended studies and collaborative projects.

Validation across multiple platforms demonstrates this antibody's versatility in your workflow. Western blot analysis at 1:1000 dilution reveals a band at approximately 104 kDa across diverse human cell lines including HeLa, A549, K562, Jurkat, U251, COLO205, and THP-1—the slight difference from the predicted 100 kDa molecular weight likely reflects post-translational modifications such as glycosylation and phosphorylation. Immunohistochemistry studies confirm reliable staining in paraffin-embedded human kidney tissue, while immunofluorescence analysis in A549 cells and flow cytometry validation in A431 cells further establish its utility for cellular localization and quantitative expression studies.

Whether investigating nuclear transport dynamics, splicing factor regulation, or viral-host interactions, this antibody provides a dependable tool for researchers exploring TNPO3 biology across multiple experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Importin, which transports target proteins into the nucleus. Specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1 and SFRS2, by recognizing phosphorylated SR domains. Also mediates the nuclear import of serine/arginine (SR) protein CPSF6, independently of CPSF6 phosphorylation. The nuclear import process is regulated by the small GTPase Ran that partitions between cytoplasm and nucleus in the predominantly GDP- and GTP-bound form, respectively. Importin associates with target cargo proteins in the cytoplasm, and the competitive binding of GTP-bound Ran induces the release of cargos in the nucleus.; (Microbial infection) Involved in immunodeficiency virus (HIV-1) infection by importing the pre-integration complex (PIC) into the nucleus. Required for a nuclear maturation step of HIV-1 prior to integration.

Gene References into Functions

Combining these results with small-angle X-ray scattering data for the complex of TRN-SR2 with truncated integrase, we propose a molecular model of the complex. We speculate that nuclear import of the PIC may proceed concurrently with the normal nuclear transport. PMID: 28356354
IRF5-TNPO3 genetic variation is associated systemic lupus erythematosus. PMID: 25205108
Data suggest that the HIV-1 integrase (IN)/transportin-SR2 (TRN-SR2) interaction interface is a potential target for antiviral therapy. PMID: 25063804
Tnpo3 mutants that are not able to interact with cleavage and polyadenylation specificity factor 6 do not facilitate HIV-1 infectivity, suggesting a potential route of pharmacological intervention in the treatment of AIDS. PMID: 24449914
TNPO3 mutation is the cause of limb-girdle muscular dystrophy 1F, expands knowledge of the molecular basis of muscular dystrophies and bolsters the importance of defects of nuclear envelope proteins as causes of inherited myopathies. PMID: 23543484
The TNPO3 gene is mapped within the Limb-girdle muscular dystrophy 1F critical interval and its 923-amino acid human gene product is also expressed in skeletal muscle. PMID: 23667635
a model wherein one monomer of TRN-SR2 is bound to one monomer of RanGTP. PMID: 23878195
In skeletal muscle of limb-girdle muscular dystrophy 1F individuals, expression of transportin 3 indicates altered transportin 3 function. PMID: 23543484
These results suggest that TNPO3 and cyclophilin A facilitate HIV-1 infection by coordinating proper uncoating of the core in target cells. PMID: 23097435
Identification of residues in the C-terminal domain of HIV-1 integrase that mediate binding to the transportin-SR2 protein. PMID: 22872638
TNPO3 can directly engage the HIV-1 IN tetramer prebound to the cognate DNA. PMID: 22872640
Transportin 3 and importin alpha act as receptors and are required for effective nuclear import of HIV-1 integrase in virus-infected cells. PMID: 21326825
TNPO3 interacts with HIV-1 gag in the cytoplasm to assist HIV-1 infection after nuclear import. PMID: 22398280
TNPO3 promotes HIV-1 infectivity at a step in the virus life cycle that is detectable after the preintegration complex arrives in the nucleus and capsid is the viral determinant for TNPO3 dependence. PMID: 22145813
TNPO3 binds to a surface of monomeric HIV-1 integrase that remains exposed after tetramerization. PMID: 22176773
a novel nuclear localization signal and mechanism for serpinF1 nuclear import PMID: 22028839
The authors demonstrated that TNPO3 was required by several lentiviruses for nuclear import. PMID: 21976643
Tnp3 binds to tRNAs and capsids proteins, and is required for efficient HIV-1 integration. PMID: 21901095
Here the authors discover a link between the viral entry of HIV and its interaction with TRN-SR2. PMID: 21276267
capsid, not integrase, is the dominant viral factor that dictates transportin 3 dependency during HIV-1 infection PMID: 19846519
Results imply that the mechanism by which SR proteins are imported to the nucleus is conserved between Drosophila and humans and involves the nuclear import receptor transportin-SR PMID: 12134081

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Involvement in disease

Limb-girdle muscular dystrophy 1F (LGMD1F)

Subcellular Location

Nucleus envelope. Cytoplasm.

Tissue Specificity

Expressed in skeletal muscle.

Database Links

HGNC: 17103

OMIM: 608423

KEGG: hsa:23534

STRING: 9606.ENSP00000265388

UniGene: Hs.193613

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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