Recombinant Human Tripartite motif-containing protein 16 (TRIM16)

Code CSB-YP024453HU
MSDS
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Source Yeast
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Code CSB-EP024453HU
MSDS
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Source E.coli
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Code CSB-EP024453HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP024453HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP024453HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TRIM16
Uniprot No.
Alternative Names
EBBP; Estrogen responsive B box protein ; Estrogen-responsive B box protein; TRI16_HUMAN; TRIM 16; TRIM16; Tripartite motif containing 16; Tripartite motif containing protein 16; Tripartite motif-containing protein 16
Species
Homo sapiens (Human)
Expression Region
1-564
Target Protein Sequence
MAELDLMAPG PLPRATAQPP APLSPDSGSP SPDSGSASPV EEEDVGSSEK LGRETEEQDS DSAEQGDPAG EGKEVLCDFC LDDTRRVKAV KSCLTCMVNY CEEHLQPHQV NIKLQSHLLT EPVKDHNWRY CPAHHSPLSA FCCPDQQCIC QDCCQEHSGH TIVSLDAARR DKEAELQCTQ LDLERKLKLN ENAISRLQAN QKSVLVSVSE VKAVAEMQFG ELLAAVRKAQ ANVMLFLEEK EQAALSQANG IKAHLEYRSA EMEKSKQELE RMAAISNTVQ FLEEYCKFKN TEDITFPSVY VGLKDKLSGI RKVITESTVH LIQLLENYKK KLQEFSKEEE YDIRTQVSAV VQRKYWTSKP EPSTREQFLQ YAYDITFDPD TAHKYLRLQE ENRKVTNTTP WEHPYPDLPS RFLHWRQVLS QQSLYLHRYY FEVEIFGAGT YVGLTCKGID RKGEERNSCI SGNNFSWSLQ WNGKEFTAWY SDMETPLKAG PFRRLGVYID FPGGILSFYG VEYDTMTLVH KFACKFSEPV YAAFWLSKKE NAIRIVDLGE EPEKPAPSLV GTAP
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.
Gene References into Functions
  1. High TRIM16 expression is associated with drug resistance in Non-Small Cell Lung Cancer. PMID: 29295721
  2. TRIM16 inhibits the migration and invasion via suppressing the Sonic hedgehog signaling pathway in ovarian cancer cells. PMID: 27737724
  3. The cooperation between TRIM16 and Galectin-3 in targeting and activation of selective autophagy protects cells from lysosomal damage and Mycobacterium tuberculosis invasion. PMID: 27693506
  4. TRIM16 expression is decreased in prostate cancer tissues and overexpression of TRIM16 inhibits cell migration, invasion and the EMT process in vitro in prostate cancer through the transcription factor Snail. PMID: 27748839
  5. Results found that the expression of TRIM16 was significantly downregulated in hepatocellular carcinoma cell (HCC) lesions and define TRIM16 as an inhibitor of epithelial-mesenchymal transition and metastasis in HCC. PMID: 26892350
  6. TRIM16 directly regulated the degradation of Gli1 protein via the ubiquitinproteasome pathway. TRIM16 expression was low in breast cancer. It negatively correlated with metastasis and suppressed stem-cell properties in breast cancer cells. PMID: 26718507
  7. Data suggest that TRIM16 and TDP43 are both good prognosis indicators; data shows that TRIM16 inhibits cancer cell viability by a novel mechanism involving interaction and stabilisation of TDP43 with consequent effects on E2F1 and pRb proteins. PMID: 26902425
  8. Expression of SDMGC and TRIM16 was upregulated in the distant metastasis tissues PMID: 25866896
  9. Chromatin immunoprecipitation assays revealed TRIM16 directly bound the IFNbeta1 gene promoter. Low level TRIM16 expression in 91 melanoma patient samples, strongly correlated with lymph node metastasis, and, predicted poor patient prognosis. PMID: 25333256
  10. results suggest that TRIM16 is a potential pharmacologic target for the treatment of NSCLC and promotion TRIM16 expression might represent a novel strategy to NSCLC metastasis PMID: 25843803
  11. TRIM16 can promote apoptosis by directly modulating caspase-2 activity in cancer cells. PMID: 23404198
  12. data suggest that TRIM16 acts as a novel regulator of both neuroblastoma G 1/S progression and cell differentiation PMID: 23422002
  13. via its unique structure, TRIM16 possesses both heterodimerization function with other TRIM proteins and also has E3 ubiquitin ligase activity. PMID: 22629402
  14. TRIM16 acts as a tumour suppressor, affecting neuritic differentiation, cell migration and replication through interactions with cytoplasmic vimentin and nuclear E2F1 in neuroblastoma cells. PMID: 20729920
  15. role in regulating keratinocyte differentiation PMID: 11919186
  16. These results provide evidence for a role of EBBP in innate immunity by enhancing the alternative secretion pathway of IL-1beta. PMID: 16575408
  17. EBBP increased betaRARE-transactivating function through its coiled-coil domain PMID: 16636064
  18. The estrogen-responsive B box protein (EBBP) restores retinoid sensitivity in retinoid-resistant cancer cells via effects on histone acetylation. PMID: 19147277

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Subcellular Location
Cytoplasm.
Protein Families
TRIM/RBCC family
Tissue Specificity
Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.
Database Links

HGNC: 17241

OMIM: 609505

KEGG: hsa:10626

STRING: 9606.ENSP00000338989

UniGene: Hs.123534

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