Recombinant Human Tripartite motif-containing protein 4 (TRIM4)

Code CSB-YP866336HU
MSDS
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Source Yeast
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Code CSB-EP866336HU
MSDS
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Source E.coli
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Code CSB-EP866336HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866336HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP866336HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TRIM4
Uniprot No.
Alternative Names
RING finger protein 87; RNF 87; RNF87; TRIM4; TRIM4_HUMAN; Tripartite motif containing 4; Tripartite motif protein 4; Tripartite motif protein TRIM4; Tripartite motif-containing protein 4
Species
Homo sapiens (Human)
Expression Region
1-500
Target Protein Sequence
MEAEDIQEEL TCPICLDYFQ DPVSIECGHN FCRGCLHRNW APGGGPFPCP ECRHPSAPAA LRPNWALARL TEKTQRRRLG PVPPGLCGRH WEPLRLFCED DQRPVCLVCR ESQEHQTHAM APIDEAFESY RTGNFDIHVD EWKRRLIRLL LYHFKQEEKL LKSQRNLVAK MKKVMHLQDV EVKNATQWKD KIKSQRMRIS TEFSKLHNFL VEEEDLFLQR LNKEEEETKK KLNENTLKLN QTIASLKKLI LEVGEKSQAP TLELLQNPKE VLTRSEIQDV NYSLEAVKVK TVCQIPLMKE MLKRFQVAVN LAEDTAHPKL VFSQEGRYVK NTASASSWPV FSSAWNYFAG WRNPQKTAFV ERFQHLPCVL GKNVFTSGKH YWEVESRDSL EVAVGVCRED VMGITDRSKM SPDVGIWAIY WSAAGYWPLI GFPGTPTQQE PALHRVGVYL DRGTGNVSFY SAVDGVHLHT FSCSSVSRLR PFFWLSPLAS LVIPPVTDRK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin-protein ligase. Mediates 'Lys-63'-linked polyubiquitination of the innate immune receptor DDX58, this linkage doesn't lead to proteasomal degradation but seems to enhance IFN induction.
Gene References into Functions
  1. TRIM4 is an important regulator of the virus-induced interferon induction pathways by mediating RIG-I for K63-linked ubiquitination. PMID: 24755855
  2. ZNF198-FGFR1 is associated with phosphorylation of several proteins including SSBP2, ABL, FLJ14235, CALM and TRIM4 proteins. PMID: 19658100
Subcellular Location
Cytoplasm.
Protein Families
TRIM/RBCC family
Database Links

HGNC: 16275

KEGG: hsa:89122

STRING: 9606.ENSP00000348216

UniGene: Hs.50749

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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