Name: Recombinant Human Ubiquitin carboxyl-terminal hydrolase 10 (USP10)
Brand: CUSABIO
SKU: 613516HU

Product Details

Purity

>85% (SDS-PAGE)

Target Names

USP10

Uniprot No.

Q14694

Alternative Names

Deubiquitinating enzyme 10; KIAA0190; MGC124997; MGC2621; Ubiquitin carboxyl terminal hydrolase 10; Ubiquitin carboxyl-terminal hydrolase 10; Ubiquitin specific peptidase 10; Ubiquitin specific processing protease 10; Ubiquitin specific protease 10; Ubiquitin thioesterase 10; Ubiquitin thiolesterase 10; Ubiquitin-specific-processing protease 10; UBP10_HUMAN; UBPO; USP 10; USP10

Species

Homo sapiens (Human)

Expression Region

2-798

Target Protein Sequence

ALHSPQYIF GDFSPDEFNQ FFVTPRSSVE LPPYSGTVLC GTQAVDKLPD GQEYQRIEFG VDEVIEPSDT LPRTPSYSIS STLNPQAPEF ILGCTASKIT PDGITKEASY GSIDCQYPGS ALALDGSSNV EAEVLENDGV SGGLGQRERK KKKKRPPGYY SYLKDGGDDS ISTEALVNGH ANSAVPNSVS AEDAEFMGDM PPSVTPRTCN SPQNSTDSVS DIVPDSPFPG ALGSDTRTAG QPEGGPGADF GQSCFPAEAG RDTLSRTAGA QPCVGTDTTE NLGVANGQIL ESSGEGTATN GVELHTTESI DLDPTKPESA SPPADGTGSA SGTLPVSQPK SWASLFHDSK PSSSSPVAYV ETKYSPPAIS PLVSEKQVEV KEGLVPVSED PVAIKIAELL ENVTLIHKPV SLQPRGLINK GNWCYINATL QALVACPPMY HLMKFIPLYS KVQRPCTSTP MIDSFVRLMN EFTNMPVPPK PRQALGDKIV RDIRPGAAFE PTYIYRLLTV NKSSLSEKGR QEDAEEYLGF ILNGLHEEML NLKKLLSPSN EKLTISNGPK NHSVNEEEQE EQGEGSEDEW EQVGPRNKTS VTRQADFVQT PITGIFGGHI RSVVYQQSSK ESATLQPFFT LQLDIQSDKI RTVQDALESL VARESVQGYT TKTKQEVEIS RRVTLEKLPP VLVLHLKRFV YEKTGGCQKL IKNIEYPVDL EISKELLSPG VKNKNFKCHR TYRLFAVVYH HGNSATGGHY TTDVFQIGLN GWLRIDDQTV KVINQYQVVK PTAERTAYLL YYRRVDLL

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

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Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I have a question about the phosphatase treatment. Would it be possible to add phosphatase inhibitor treatment during lysis (e.g. in the lysis buffer). Exemplarily, you could take the phosphatase inhibitor cocktail from Sigma (link below):
https://www.sigmaaldrich.com/DE/de/product/sigma/p2850
Could you specify the buffer composition that you will use for the final purification? We are using a 50 mM HEPES, 200 mM NaCl buffer with 0.4 mM TCEP.

We can add phosphatase inhibitor treatment during lysis, please remark this requirement when placing an order.
The buffer composition that we will use for the final purification will depend on the specific experiment. We usually use Tris or PBS buffer, and we can also use HEPES buffer according to customer's requirement. We will try the 50 mM HEPES, 200 mM NaCl buffer with 0.4 mM TCEP first. If the finally buffer is different from that specified by the customer, we will communicate with you in advance.

For the mammalian cell protein, does your lab use protease inhibitors during production/purification?

Regarding CSB-MP613516HU, the mammalian cell protein, protease inhibitors are usually used in the process of reproduction and purification to prevent protein degradation.

I would like to know if the protease inhibitors that you are using include phosphatase inhibitors.

Protease inhibitors are commonly used in the production and purification of our proteins expressed by mammalian cells, such as PMSF and a comprehensive protease inhibitor.

Target Background

Function

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Deubiquitinates TBX21 leading to its stabilization.

Gene References into Functions

By using a genome wide siRNA screen for deubiquitinating enzymes, we identified USP10 as a deubiquitinase for Slug in cancer cells. USP10 interacts with Slug and mediates its degradation by the proteasome. Importantly, USP10 is concomitantly highly expressed with Slug in cancer biopsies. PMID: 29803676
Loss of USP10 expression is associated with small intestinal adenocarcinoma. PMID: 30375264
One of the USP10 targets is TP53. Wildtype TP53 is usually rescued from proteasomal degradation by USP10. As most KMT2A leukemias display wildtype p53 alleles, one might argue that the disruption of an USP10 allele can be classified as a pro-oncogenic event. PMID: 30107050
Deubiquitylase USP10 interacts with RNF168 and TOP2alpha, and restrains ubiquitylation of TOP2alpha as well as its chromatin binding. PMID: 27558965
USP10 directly interacted with and stabilized PTEN via deubiquitination. PMID: 28852924
A controlling role for USP10 after wounding in determining myofibroblast development and activation of fibrotic TGFbeta signaling. PMID: 28851806
Investigate the expression of USP10 in the human normal adrenal gland and various adrenal tumors. In adrenal tumors, detectable levels of USP10 protein were found in 100 % (30/30) adrenocortical adenomas, 88.89 % (8/9) adrenocortical carcinomas, and 10 % (2/20) pheochromocytomas. PMID: 26555087
G3BP mediates the condensation of stress granules by shifting between two different states that are controlled by the phosphorylation of S149 and by binding to Caprin1 or USP10. PMID: 27022092
Data demonstrated that AMPK-USP10 form a positive feedforward loop that ensures amplification of AMPK activation in response to fluctuation of cellular energy status. PMID: 26876938
miR-191 could promote pancreatic cancer progression through targeting USP10, implicating a novel mechanism for the tumorigenesis. PMID: 25168367
USP10 deubiquitinates and stabilizes SIRT6. PMID: 24332849
this study identified USP10, a carboxyl-terminal ubiquitin-processing protease, could interact with T-bet in the nucleus. PMID: 24845384
USP10 is a host factor that inhibits stress-induced reactive oxygen species production and apoptosis in HTLV-1-infected T cells. PMID: 23775713
a novel function for USP10 in facilitating the deubiquitination of CFTR in early endosomes, thereby enhancing the endocytic recycling and cell surface expression of CFTR. PMID: 20215869
Findings reveal USP10 to be a novel regulator of p53, providing an alternative mechanism of p53 inhibition in cancers with wild-type p53. PMID: 20096447
The data indicate that USP10 is a new cofactor that binds to the androgen receptor (AR) and stimulates the androgen response of target promoters. This finding underlines the role of the ubiquitin/proteasome system in modulating the AR function. PMID: 16368182
RTQ-LDA and RTQ identified ubiquitin specific protease 10 as significantly over-expressed in dead-of-disease compared to long-term survival glioblastoma multiforme patients. PMID: 16773218
USP10 has a role in facilitating the deubiquitination of CFTR in early endosomes and thereby enhancing the endocytic recycling of CFTR PMID: 19398555
Ras-GAP SH3 domain binding protein (G3BP) is a modulator of USP10. G3BP does not appear to be a substrate of USP10; it rather inhibits its ability to disassemble ubiquitin chains. PMID: 11439350

Hide All

Subcellular Location

Cytoplasm. Nucleus. Early endosome.

Protein Families

Peptidase C19 family, USP10 subfamily

Tissue Specificity

Widely expressed.

Database Links

HGNC: 12608

OMIM: 609818

KEGG: hsa:9100

STRING: 9606.ENSP00000219473

UniGene: Hs.136778

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Recombinant Human Ubiquitin carboxyl-terminal hydrolase 10 (USP10)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

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