Product Details

Uniprot No.

Target Names

Alternative Names

Ubiquitin carboxyl-terminal hydrolase 10 (EC 3.4.19.12) (Deubiquitinating enzyme 10) (Ubiquitin thioesterase 10) (Ubiquitin-specific-processing protease 10), USP10, KIAA0190

Species Reactivity

Human

Immunogen

A synthesized peptide from human USP10 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6A4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

USP10 is a deubiquitinating enzyme that plays essential roles in cellular homeostasis by removing ubiquitin chains from substrate proteins, thereby rescuing them from proteasomal degradation. This enzyme has garnered significant research attention for its involvement in p53 stabilization, DNA damage response pathways, and autophagy regulation, making it a compelling target for studies investigating tumor suppression mechanisms and stress response signaling.

This recombinant monoclonal antibody, clone 6A4, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and production occurs through controlled expression systems, you can expect consistent performance across experiments and between lot numbers, eliminating the variability concerns that can complicate long-term studies or multi-site collaborations.

Validation studies demonstrate reliable performance in immunofluorescence and flow cytometry applications. Immunofluorescence analysis of MCF-7 breast cancer cells reveals clear cytoplasmic and nuclear staining patterns at dilutions of 1:50 to 1:200, consistent with the known shuttling behavior of USP10 between cellular compartments. Flow cytometry experiments using HeLa cells show distinct positive population shifts compared to isotype controls, confirming specific detection in fixed and permeabilized samples. These validated human cell lines provide a solid foundation for researchers working with similar experimental systems.

The unconjugated format allows flexibility in secondary antibody selection, accommodating various detection systems and multiplexing strategies. Supplied in a glycerol-containing buffer optimized for long-term storage stability, this antibody is well-suited for researchers investigating ubiquitin-proteasome system dynamics, p53 pathway regulation, or broader deubiquitinase biology in cancer and cellular stress contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Deubiquitinates TBX21 leading to its stabilization.

Gene References into Functions

By using a genome wide siRNA screen for deubiquitinating enzymes, we identified USP10 as a deubiquitinase for Slug in cancer cells. USP10 interacts with Slug and mediates its degradation by the proteasome. Importantly, USP10 is concomitantly highly expressed with Slug in cancer biopsies. PMID: 29803676
Loss of USP10 expression is associated with small intestinal adenocarcinoma. PMID: 30375264
One of the USP10 targets is TP53. Wildtype TP53 is usually rescued from proteasomal degradation by USP10. As most KMT2A leukemias display wildtype p53 alleles, one might argue that the disruption of an USP10 allele can be classified as a pro-oncogenic event. PMID: 30107050
Deubiquitylase USP10 interacts with RNF168 and TOP2alpha, and restrains ubiquitylation of TOP2alpha as well as its chromatin binding. PMID: 27558965
USP10 directly interacted with and stabilized PTEN via deubiquitination. PMID: 28852924
A controlling role for USP10 after wounding in determining myofibroblast development and activation of fibrotic TGFbeta signaling. PMID: 28851806
Investigate the expression of USP10 in the human normal adrenal gland and various adrenal tumors. In adrenal tumors, detectable levels of USP10 protein were found in 100 % (30/30) adrenocortical adenomas, 88.89 % (8/9) adrenocortical carcinomas, and 10 % (2/20) pheochromocytomas. PMID: 26555087
G3BP mediates the condensation of stress granules by shifting between two different states that are controlled by the phosphorylation of S149 and by binding to Caprin1 or USP10. PMID: 27022092
Data demonstrated that AMPK-USP10 form a positive feedforward loop that ensures amplification of AMPK activation in response to fluctuation of cellular energy status. PMID: 26876938
miR-191 could promote pancreatic cancer progression through targeting USP10, implicating a novel mechanism for the tumorigenesis. PMID: 25168367
USP10 deubiquitinates and stabilizes SIRT6. PMID: 24332849
this study identified USP10, a carboxyl-terminal ubiquitin-processing protease, could interact with T-bet in the nucleus. PMID: 24845384
USP10 is a host factor that inhibits stress-induced reactive oxygen species production and apoptosis in HTLV-1-infected T cells. PMID: 23775713
a novel function for USP10 in facilitating the deubiquitination of CFTR in early endosomes, thereby enhancing the endocytic recycling and cell surface expression of CFTR. PMID: 20215869
Findings reveal USP10 to be a novel regulator of p53, providing an alternative mechanism of p53 inhibition in cancers with wild-type p53. PMID: 20096447
The data indicate that USP10 is a new cofactor that binds to the androgen receptor (AR) and stimulates the androgen response of target promoters. This finding underlines the role of the ubiquitin/proteasome system in modulating the AR function. PMID: 16368182
RTQ-LDA and RTQ identified ubiquitin specific protease 10 as significantly over-expressed in dead-of-disease compared to long-term survival glioblastoma multiforme patients. PMID: 16773218
USP10 has a role in facilitating the deubiquitination of CFTR in early endosomes and thereby enhancing the endocytic recycling of CFTR PMID: 19398555
Ras-GAP SH3 domain binding protein (G3BP) is a modulator of USP10. G3BP does not appear to be a substrate of USP10; it rather inhibits its ability to disassemble ubiquitin chains. PMID: 11439350

Hide All

Subcellular Location

Cytoplasm. Nucleus. Early endosome.

Protein Families

Peptidase C19 family, USP10 subfamily

Tissue Specificity

Widely expressed.

Database Links

HGNC: 12608

OMIM: 609818

KEGG: hsa:9100

STRING: 9606.ENSP00000219473

UniGene: Hs.136778

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Code	CSB-RA993982A0HU
Size	US$210
	Order now
Image	Immunofluorescence staining of MCF-7 cell with CSB-RA993982A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA993982A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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USP10 Recombinant Monoclonal Antibody

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