Recombinant Human Ubiquitin carboxyl-terminal hydrolase 20 (USP20), partial

Code CSB-YP896876HU
MSDS
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Source Yeast
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Code CSB-EP896876HU
MSDS
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Source E.coli
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Code CSB-EP896876HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896876HU
MSDS
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Source Baculovirus
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Code CSB-MP896876HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
USP20
Uniprot No.
Alternative Names
Deubiquitinating enzyme 20; hVDU2; KIAA1003; LSFR 3A; LSFR3A; pVHL interacting deubiquitinating enzyme 2; Ubiquitin carboxyl terminal hydrolase 20; Ubiquitin carboxyl-terminal hydrolase 20; ubiquitin specific peptidase 20; Ubiquitin specific processing protease 20; Ubiquitin specific protease 20; Ubiquitin thioesterase 20; Ubiquitin thiolesterase 20; Ubiquitin-specific-processing protease 20; UBP20_HUMAN; USP 20; USP20; VDU 2; VDU2; VHL interacting deubiquitinating enzyme 2; VHL-interacting deubiquitinating enzyme 2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Gene References into Functions
  1. USP20 regulates actin cytoskeleton organization and cell migration in a manner dependent on ERK3 expression. The results identify USP20 as a bona fide regulator of ERK3 stability and physiological functions. PMID: 28167606
  2. we investigated the inter-action between PKM2 and USP20. Our results suggest a new molecular pathway in cancer metabolism through the regulation of PKM2. PMID: 25708858
  3. findings reveal a dynamic regulation of USP20 by site-specific phosphorylation as well as the interdependence of signal transduction and trafficking pathways in balancing adrenergic stimulation and maintaining cellular homeostasis PMID: 25666616
  4. data establish a new function of USP20 in genome maintenance and DNA repair. PMID: 24923443
  5. Analysis of indel variations in the human disease-associated genes CDKN2AIP, WDR66, USP20 and OR7C2 in a Korean population. PMID: 22552337
  6. Our results point to USP20 as a key negative regulator of HTLV-1 Tax-induced NF-kappaB signaling. PMID: 21525354
  7. identification and description of VDU2 in human cells PMID: 12865408
  8. von Hippel-Lindau protein-interacting deubiquitinating enzyme 2 (VDU2) deubiquitinates and stabilizes HIF-1alpha. PMID: 15776016
  9. USPs 20 and 33 serve as novel regulators that dictate both post-endocytic sorting as well as the intensity and extent of beta(2)AR signalling from the cell surface. PMID: 19424180

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Subcellular Location
Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
Protein Families
Peptidase C19 family, USP20/USP33 subfamily
Database Links

HGNC: 12619

OMIM: 615143

KEGG: hsa:10868

STRING: 9606.ENSP00000313811

UniGene: Hs.5452

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