Recombinant Human Ubiquitin-conjugating enzyme E2 E1 (UBE2E1)

Code CSB-YP025448HU
MSDS
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Source Yeast
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Code CSB-EP025448HU
MSDS
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Source E.coli
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Code CSB-EP025448HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025448HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP025448HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UBE2E1
Uniprot No.
Alternative Names
UB2E1_HUMAN; UbcH6; Ube2e1; Ubiquitin carrier protein E1; Ubiquitin conjugating enzyme E2E 1 (homologous to yeast UBC4/5); Ubiquitin conjugating enzyme E2E 1; Ubiquitin-conjugating enzyme E2 E1; ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase E1
Species
Homo sapiens (Human)
Expression Region
2-193
Target Protein Sequence
SDDDSRAST SSSSSSSSNQ QTEKETNTPK KKESKVSMSK NSKLLSTSAK RIQKELADIT LDPPPNCSAG PKGDNIYEWR STILGPPGSV YEGGVFFLDI TFTPEYPFKP PKVTFRTRIY HCNINSQGVI CLDILKDNWS PALTISKVLL SICSLLTDCN PADPLVGSIA TQYMTNRAEH DRMARQWTKR YAT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.
Gene References into Functions
  1. UBE2E1 expression was adversely correlated with AML survival; UBE2E1 expression was negatively correlated with patients' response to induction chemotherapy PMID: 27855695
  2. IPO11 provides protection in another way, the researchers found. The transport receptor also shuttles UBE2E1, an enzyme necessary for PTEN ubiquitination, into the nucleus. Thanks to IPO11, normal cells keep all of their UBE2E1 in the nucleus, allowing PTEN to continue functioning in the cytoplasm. In cells lacking IPO11, UBE2E1 remains in the cytoplasm and promotes the destruction of PTEN. PMID: 28258103
  3. study reveals that UbE2E1 is an in vivo E2 for the PRC1 ligase complex and thus plays an important role in the regulation of H2A Lys-119 monoubiquitination PMID: 28073915
  4. Auto-ubiquitination of Ube2E1 is intramolecular.RNF4 enhances Ube2E1 self-ubiquitination. PMID: 25960396
  5. USP7 is critical in maintaining the steady state levels of UbE2E1 in cells. PMID: 23603909
  6. isolated ISG15-modified and unmodified UbcH6 proteins, and analyzed their abilities to form thioester intermediates with ubiquitin PMID: 16428300
  7. UbcH6 and ataxin-1 are E2-substrate cognate pairs in the ubiquitin-proteasome system. PMID: 18439907
  8. UbcH6 modulates the transcriptional repression activity of ataxin-1 by modulating the degradation of ataxin-1. PMID: 18519031
  9. E1 ligase of the proteasome is inhibitd by ziram and has a role in preventing dopaminergic cell damage PMID: 18818210
  10. Smallangle X-ray scattering experiments show that the Lys63 tetra-Ub chain is dynamic in solution, adopting an ensemble of conformations that are more compact than the extended form in the crystal. PMID: 19664638

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Subcellular Location
Nucleus.
Protein Families
Ubiquitin-conjugating enzyme family
Database Links

HGNC: 12477

OMIM: 602916

KEGG: hsa:7324

STRING: 9606.ENSP00000303709

UniGene: Hs.164853

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