Recombinant Human Uroporphyrinogen decarboxylase (UROD)

Code CSB-YP025678HU
MSDS
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Source Yeast
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Code CSB-EP025678HU
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Source E.coli
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Code CSB-EP025678HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025678HU
MSDS
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Source Baculovirus
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Code CSB-MP025678HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UROD
Uniprot No.
Alternative Names
DCUP_HUMAN; PCT; UPD; URO D; URO-D; urod; Uroporphyrinogen decarboxylase; Uroporphyrinogen III decarboxylase
Species
Homo sapiens (Human)
Expression Region
1-367
Target Protein Sequence
MEANGLGPQG FPELKNDTFL RAAWGEETDY TPVWCMRQAG RYLPEFRETR AAQDFFSTCR SPEACCELTL QPLRRFPLDA AIIFSDILVV PQALGMEVTM VPGKGPSFPE PLREEQDLER LRDPEVVASE LGYVFQAITL TRQRLAGRVP LIGFAGAPWT LMTYMVEGGG SSTMAQAKRW LYQRPQASHQ LLRILTDALV PYLVGQVVAG AQALQLFESH AGHLGPQLFN KFALPYIRDV AKQVKARLRE AGLAPVPMII FAKDGHFALE ELAQAGYEVV GLDWTVAPKK ARECVGKTVT LQGNLDPCAL YASEEEIGQL VKQMLDDFGP HRYIANLGHG LYPDMDPEHV GAFVDAVHKH SRLLRQN
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.
Gene References into Functions
  1. a new mutation in the UROD gene in Egyptian patients with Hepatoerythropoietic porphyria, is reported. PMID: 26789143
  2. a new insight in the conformational changes occurred in the mutant structures of UROD protein. PMID: 24777812
  3. Data suggest that the traditional Chinese medicine (TCM) candidate potential three-in-one inhibitors for three drug target proteins epidermal growth factor receptor (EGFR), Her2, and uroporphyrinogen decarboxylase (UROD) against head and neck cancer. PMID: 23140436
  4. Expression of the D306Y mutation results in an insoluble recombinant protein. G318R and K297N have little effect on the structure or activity of recombinant URO-D, but the proteins display reduced stability in vitro. PMID: 19656450
  5. Among seventeen F-PCT patients, sixteen UROD mutations were identified. PMID: 23545314
  6. analysis of uroporphyrinogen decarboxylase as a potential target for specific components of traditional Chinese medicine PMID: 23209648
  7. Mutations in UROD gene is associated with familial porphyria cutanea tarda. PMID: 22382040
  8. Hepatoerythropoietic porphyria and familial porphyria cutanea tarda G281E mutation in the uroporphyrinogen decarboxylase gene PMID: 21079081
  9. function of Arg37 in uroporphyrinogen III decarboxylase PMID: 20553007
  10. description of 3 siblings with hepatoerythropoietic porphyria; sequencing of the UROD gene revealed compound heterozygosity for a novel missense mutation, V166A, and a complex deletion/insertion, 645del1053ins10 PMID: 20479301
  11. Sequence deletions in uroporphyrinogen decarboxylase is associated with Porphyria Cutanea Tarda. PMID: 15046048
  12. we identified eight mutations in 18 previously unclassified porphyria cutanea tarda families PMID: 15186324
  13. Sequencing of the hydroxymethylbilane synthase and uroporphyrinogen decarboxylase genes confirmed the relatively rare diagnosis of dual porphyria, and revealed a novel uroporphyrinogen decarboxylase mutation PMID: 16390615
  14. There is a high degree of molecular heterogeneity of familial porphyria cutanea tarda in Spain and molecular genetic analysis is useful in distinguish between F-PCT and sporadic PCT. PMID: 17627795
  15. role of UROD mutations as a strong risk factor for porphyria cutanea tarda even in areas where environmental factors (hepatitis C virus) have been shown to be highly associated with the disease PMID: 19419417

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Involvement in disease
Familial porphyria cutanea tarda (FPCT); Hepatoerythropoietic porphyria (HEP)
Subcellular Location
Cytoplasm.
Protein Families
Uroporphyrinogen decarboxylase family
Database Links

HGNC: 12591

OMIM: 176100

KEGG: hsa:7389

STRING: 9606.ENSP00000246337

UniGene: Hs.78601

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