Recombinant Human Vesicle-associated membrane protein-associated protein A (VAPA), partial

Code CSB-YP878947HU
MSDS
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Source Yeast
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Code CSB-EP878947HU
MSDS
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Source E.coli
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Code CSB-EP878947HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878947HU
MSDS
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Source Baculovirus
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Code CSB-MP878947HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VAPA
Uniprot No.
Alternative Names
33 kDa Vamp-associated protein; hVAP 33; MGC3745; OTTHUMP00000162362; OTTHUMP00000162363; VAMP (vesicle-associated membrane protein) associated protein A, 33kDa; VAMP A; VAMP associated protein A; VAMP-A; VAMP-associated protein A; VAP 33; VAP A; VAP-33; VAP-A; VAP33; Vapa; VAPA_HUMAN; Vesicle associated membrane protein associated protein A; Vesicle-associated membrane protein-associated protein A
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to OSBPL3, which mediates recruitment of VAPA to plasma membrane sites. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With OSBPL3, may regulate ER morphology. May play a role in vesicle trafficking.
Gene References into Functions
  1. The effects of sterol manipulation of HuH7 cells on complexes of established sterol-binding Oxysterol-binding protein related proteins with their endoplasmic reticulum receptor, VAMP-associated protein A (VAPA), were examined. PMID: 25681634
  2. phosphorylation of ORP3 controls its association with VAPA. Furthermore, ORP3-VAPA complexes stimulate R-Ras signaling. PMID: 25447204
  3. GPS2 is required for the association of viral NS5A with VAP-A and hepatitis C virus replication. PMID: 24223774
  4. STARD3 or STARD3NL and VAP form a novel molecular tether between late endosomes and the endoplasmic reticulum. PMID: 24105263
  5. phosphorylation of CERT at the FFAT motif-adjacent serine affects its affinity for VAP, which may regulate the inter-organelle trafficking of ceramide in response to the perturbation of cellular sphingomyelin and/or other sphingolipids PMID: 24569996
  6. Viperin inhibits hepatitis C virus replication by interfering with binding of NS5A to host protein VAP-33. PMID: 21957124
  7. Data show that the methylated VAPA-APCDD1 DNA in maternal plasma is predominantly derived from the fetus, and this novel fetal epigenetic marker in maternal plasma is useful for the noninvasive detection of fetal trisomy 18. PMID: 21152411
  8. Electrostatic interaction between oxysterol-binding protein and VAMP-associated protein A revealed by NMR and mutagenesis studies. PMID: 20178991
  9. protein-protein interactions among the various HCV NS proteins and hVAP-33 are important for the formation of HCV replication complex PMID: 15016871
  10. Our results provide suggestive evidence of an association between SNPs in the 3'UTR of the VAPA gene and bipolar disorder. PMID: 18665321
  11. VAP-A is an important regulator both of the subcellular localization of protrudin and of its ability to stimulate neurite outgrowth. PMID: 19289470
  12. GLTP and VAP-A were shown to interact. PMID: 19665998

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Subcellular Location
Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Cell membrane; Single-pass type IV membrane protein. Cell junction, tight junction. Nucleus membrane.
Protein Families
VAMP-associated protein (VAP) (TC 9.B.17) family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 12648

OMIM: 605703

KEGG: hsa:9218

UniGene: Hs.165195

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