Recombinant Human Wiskott-Aldrich syndrome protein family member 2(WASF2)

Code CSB-YP896553HU
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Source Yeast
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Code CSB-EP896553HU
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Source E.coli
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Code CSB-EP896553HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896553HU
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Source Baculovirus
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Code CSB-MP896553HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names WASF2
Uniprot No. Q9Y6W5
Alternative Names DICTYOSTELIUM; dJ393P12.2; IMD2; Protein WAVE-2; Putative Wiskott Aldrich syndrome protein family member 4; SCAR 2; SCAR; SCAR2; Suppressor of cyclic-AMP receptor (WASP family); Verprolin homology domain-containing protein 2; WASF2; WASF2_HUMAN; WASF4; WASP family protein member 2; WASP family protein member 4; WASP family Verprolin homologous protein 2; WASP-family protein member 2; WAVE 2; WAVE2; Wiskott-Aldrich syndrome protein family member 2; Wiskott-Aldrich syndrome protein family verprolin-homologous protein
Species Homo sapiens (Human)
Expression Region 1-498
Target Protein Sequence MPLVTRNIEP RHLCRQTLPS VRSELECVTN ITLANVIRQL GSLSKYAEDI FGELFTQANT FASRVSSLAE RVDRLQVKVT QLDPKEEEVS LQGINTRKAF RSSTIQDQKL FDRNSLPVPV LETYNTCDTP PPLNNLTPYR DDGKEALKFY TDPSYFFDLW KEKMLQDTKD IMKEKRKHRK EKKDNPNRGN VNPRKIKTRK EEWEKMKMGQ EFVESKEKLG TSGYPPTLVY QNGSIGCVEN VDASSYPPPP QSDSASSPSP SFSEDNLPPP PAEFSYPVDN QRGSGLAGPK RSSVVSPSHP PPAPPLGSPP GPKPGFAPPP APPPPPPPMI GIPPPPPPVG FGSPGTPPPP SPPSFPPHPD FAAPPPPPPP PAADYPTLPP PPLSQPTGGA PPPPPPPPPP GPPPPPFTGA DGQPAIPPPL SDTTKPKSSL PAVSDARSDL LSAIRQGFQL RRVEEQREQE KRDVVGNDVA TILSRRIAVE YSDSEDDSSE FDEDDWSD
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Gene References into Functions
  1. Results show that in cisplatin-resistant cervical cancer tissues, Rac1, and Wave2 mRNA expression was significantly up-regulated compared to that of the cisplatin-sensitive cervical cancer tissues. In HeLa and Caski cervical cancer cell lines, Rac1 activity and Wave2 protein expression was significantly promoted by SH3BP1 overexpression. PMID: 28786507
  2. Studied leucine rich repeat kinase 2 (LRRK2)-WAS protein family member 2 (WAVE2) pathway in modulation of phagocytosis in leukocytes, as well as its possible role for altered immune function in Parkinson's Disease. PMID: 29760073
  3. The level of WASF2 in gastric cancer tissues was negatively correlated with miR-146a expression and had inverse clinicopathologic features. The newly identified miR-146a/WASF2 axis may provide a novel therapeutic target for gastric cancer. PMID: 28387985
  4. SH3BP1 promoted VEGF secretion via Rac1-WAVE2 signaling, so as to exert an augmentation on cell invasion and microvessel formation. PMID: 26933917
  5. the MCP1-induced cortactin phosphorylation is dependent on PLCb3-mediated PKC activation, and siRNA-mediated down-regulation of either of these molecules prevents cortactin interaction with WAVE2 PMID: 26490115
  6. WASp and WAVE2 differ in their dynamics and their associated proteins PMID: 25342748
  7. Decreased expression of Wiskott-Aldrich syndrome protein family verprolin-homologous protein 2 may be involved in the development of pre-eclampsia. PMID: 24125947
  8. these results suggest that Arp2/3 and the upstream regulator, WAVE2, are essential co-factors hijacked by HIV for intracellular migration, and may serve as novel targets to prevent HIV transmission. PMID: 24415754
  9. WAVE2 is down-regulated in gastric cancer PMID: 24778020
  10. data reveal a critical role for WAVE2 complex in regulation of cellular signaling and epithelial morphogenesis PMID: 23691243
  11. Identification of an onco-suppressive role of miR-146a in gastric cancer cells by its reduction of WASF2 expression. PMID: 23435376
  12. results suggest that SKAP2 negatively regulates cell migration and tumor invasion in fibroblasts and glioblastoma cells by suppressing actin assembly induced by the WAVE2-cortactin complex PMID: 23161539
  13. results indicate that by mediating intensive F-actin accumulation at the sites of cell infiltration, WAVE2, N-WASP, and Mena are crucial for PI3K-dependent cell invasion induced by PDGF PMID: 21769917
  14. WAVE2 and WASp define parallel pathways to F-actin reorganization and function in NK cells; although WAVE2 was not required for NK cell innate function, it was accessible through adaptive immunity via IL-2. PMID: 21383498
  15. Data suggest that ERK coordinates adhesion disassembly with WAVE2 regulatory complex activation and actin polymerization to promote productive leading edge advancement during cell migration. PMID: 21419341
  16. WAVE2 works as an A-kinase-anchoring protein that recruits PKA at membrane protrusions and plays a role in the enlargement of membrane protrusions induced by PKA activation PMID: 21119216
  17. MYH9 is required for lamellipodia formation since it provides contractile forces and tension for the F-actin network to form convex arcs at the leading edge through constitutive binding to WAVE2 and colocalization with PIP(3) in response to IGF-I. PMID: 21184743
  18. WAVE2 complexs with insulin receptor substrate p53 and is crucial for membrane targeting followed by local accumulation of WAVE2 at the leading edge of cells. PMID: 20621182
  19. WAVE2-membrane targeting is directionally controlled by binding of the EB1-stathmin complex to WAVE2-bearing microtubules and by the interaction between WAVE2 and PIP3 produced near IGF-IR that is locally activated by IGF-I. PMID: 19925864
  20. The WAVE2-Abi1-Nap1-PIR121 complex is as active as the WAVE2-Abi1 sub-complex in stimulating Arp2/3, and after Rac activation it is re-localized to the leading edge of ruffles in vivo. PMID: 15048123
  21. reconstitution of the Wave-2 complex and a study of the molecular architecture PMID: 15070726
  22. Results suggest that phosphatidylinositol (3,4,5)-triphosphate recruits WAVE2 to the polarized membrane and that this recruitment is essential for lamellipodium formation at the leading edge. PMID: 15107862
  23. the 3 WAVE isoforms exhibit common and distinct features and may potentially be involved in the regulation of actin cytoskeleton in platelets PMID: 15280206
  24. WAVE-2 and WAVE-3 expression was not changed by the megakaryocytic differentiation; WAVE-1 and WAVE-2 moved from a detergent-soluble cytosolic fraction to insoluble cytoskeleton fraction after platelet aggregation PMID: 15670045
  25. WAVE2 is a key component of the actin regulatory machinery in T cells and that it also participates in linking intracellular calcium store depletion to calcium release-activated calcium (CRAC) channel activation. PMID: 16401421
  26. Therefore, IRSp53 optimizes the activity of the WAVE2 complex in the presence of activated Rac and PIP(3). PMID: 16702231
  27. WAVE2 expression is elevated in HCC tissues, which correlates with a poor prognosis, suggesting WAVE2 as a candidate prognostic marker of HCC. PMID: 17020969
  28. Colocalization of Arp2 and WAVE2 is an independent risk factor for liver metastasis of colorectal carcinoma. PMID: 17459058
  29. Results decribe a mechanism in which signals from the T cell receptor produce WAVE2-ARP2/3-mediated de novo actin polymerization, leading to integrin clustering and high-affinity binding through the recruitment of vinculin and talin. PMID: 17591693
  30. Over-expression of WAVE2 is seen in node-positive cases as well as in moderately and poorly differentiated breast cancers. PMID: 18509249
  31. Data suggest that the promotion of lamellipodia formation by HGF in MDA-MB-231 cells is Rac1-dependent and requires KIF5B-mediated transport of WAVE2 and IQGAP1 to the cell periphery along microtubules. PMID: 18514191
  32. results suggest that long protrusions formed in a WAVE2- and microtubule-dependent manner may identify the cells at the later stage of invasion, possibly after the formation of invadopodia in the 3-D cultures PMID: 18795939
  33. Results identify a mechanism by which the WAVE2 complex regulates T cell receptor signaling to Rap1 and integrin activation via Abl- and CrkL-C3G. PMID: 18809728
  34. Phosphorylation of all five sites on endogenous WAVE2 and their mutation to non-phosphorylatable alanine residues leads to inhibition of WAVE2 function in vivo, was demonstrated. PMID: 19012317
  35. Pak1 protein binds to WASF2 protein and is transported with WASF2 protein to the leading edge by stimulation with hepatocyte growth factor. PMID: 19162178
  36. betaPIX and GIT1 regulate the hepatocyte growth factor-induced and Rac1-dependent membrane transport of WAVE2 and consequently, lamellipodia formation. PMID: 19303398
  37. HSPC300 is associated with metastatic potential of lung squamous cell carcinoma through its interaction with WAVE2 PMID: 19576655
  38. Data show that activation of the WAVE2 complex requires simultaneous interactions with prenylated Rac-GTP and acidic phospholipids, as well as a specific state of phosphorylation. PMID: 19917258

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Subcellular Location Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Basolateral cell membrane.
Protein Families SCAR/WAVE family
Tissue Specificity Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.
Database Links

HGNC: 12733

OMIM: 605875

KEGG: hsa:10163

STRING: 9606.ENSP00000396211

UniGene: Hs.469244

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